Effects of Granulocyte-Colony Stimulating Factor on Wound Healing in a Mouse Model of Burn Trauma

Abstract

The effects of burn trauma and granulocyte-colony stimulating factor (G-CSF) treatment on wound healing in a surgical incision model were studied. Sixty adult male mice were used in this study. Under general anesthesia hot water at 97°C was applied for 3 sec to the dorsum of the mice in order to achieve 20% burn wound. After burn trauma, full thickness midline skin incision 2 cm in length was performed on the abdominal wall and then were sutured primarily with 4/0 polypropylene. In Group I only skin incision was performed, group II had skin incision and burn, in group III G-CSF (0.03 BU/30 g) was applied intraperitoneally after burn and skin incision. Breaking strength and 5-hydroxyproline (5-HP) levels of the wounds were calculated 5 and 10 days after the procedure. 5-HP levels and breaking strength values showed statistical difference between groups II-III and I-II (p<0.05). 5-HP levels were lowest in incision and burn group (41.80 μ g/mg). Breaking strength levels were also lowest in the same group (0.12 kg) (p<0.05). These results suggest that third degree burn causes a significant impairment on incisional wound healing and G-CSF ameliorates this impairment.burn; wound; wound healing; G-CSF

Use of the stem cell mobilizer SE2® as part of conventional treatment on ankle injuries to expedite recovery in professional soccer players

“…Radiological, histological and biomechanical assessment performed 3 weeks later revealed a significantly greater recovery in the G-CSF treated animals. Likewise, in mice subjected to burn and incision of the skin, animals treated with G-CSF showed greater recovery (Eroglu et al, 2004). CD34 + stem cells collected from peripheral blood following mobilization by using G-CSF were locally injected along with atelocollagen in rats subjected to medial collateral ligament injury, and results were compared with a control group only treated with atelocollagen.…”

Tratamiento complementario de heridas crónicas con factor de crecimiento estimulante de colonias de granulocitos. A propósito de dos casos clínicos.

“…(9) Posteriormente, diversos autores comenzaron a utilizar factor de crecimiento estimulante de colonias de granulocitos (G-CSF), primero en modelo animal y luego en pacientes con pie diabético infectado. (10)(11)(12)(13)(14)(15) El G-CSF, al igual que el GM-CSF, es un factor endógeno hematopoyético movilizador de células madre y progenitores hematopoyéticos de la médula ósea hacia la sangre periférica, los cuales pueden ser cuantificados mediante la determinación de células CD34+ en sangre periférica. (10,12) A nivel sistémico actúa regulando la supervivencia, proliferación y diferenciación terminal de las células precursoras de neutrófilos desde la médula a la sangre.…”

Intravenous Administration of Granulocyte-Colony Stimulating Factor for Stem Cells Mobilization and Third Degree Burn Wound Healing in Rats

“…8,16 Stem cell therapy has been established to overcome the obstacles associated with the application of skin substitutes such as difficulty and expensiveness. 17 Stem cells, especially bone marrow-derived ones, have been found to be a promising cell source for treating burn injuries in recent decades. Easy accessibility and differentiation capabilities to a variety of cell types such as blood vessel cells, perifollicular cells, perisebaceous gland cells, keratinocytes and fibroblasts, which all play vital roles in the healing process, have made stem cells a potential source for cell therapy.…”