Effects of Graded Level of Dietary L-Ascorbyl-2-Polyphosphate on Growth Performance and Some Hematological Indices of Juvenile Mahseer (Tor putitora)

Abstract

A 70 days feeding trial was conducted to evaluate the effects of graded level of L-ascorby-2-polyphosphate (APP) on growth performance and hematological indices of juvenile mahseer Tor putitora. Four different 40% protein experimental diets supplemented with APP at the rate of 0, 100, 200 and 300 mg APP kg-1 diet were fed to triplicate group of fish. The APP supplementation showed their effect in a dose dependent manner. Significantly (P < 0.05) improved growth performance i.e. weight gain (WG%), feed conversion efficiency (FCE%), specific growth rate (SGR%) and hepatosomatic index (HSI%) was observed with APP 300 , followed by APP 200 and then APP 100 enriched diet. The growth hormone (GH) level of fish also increased with increase in concentration of APP in the diet and showed a positive relationship with WG%. Moreover, the hematological parameters like red blood cell count, hemoglobin and hematocrit values also followed the same trend in response to graded levels of dietary APP supplementation, significantly (P < 0.05) higher values with APP 300 enriched diet followed by APP 200 diet while comparatively low, but significantly higher (P < 0.05) values as compared to control group of fish were obtained in response to APP 100 supplemented diets. The results of this study indicate the dose dependent beneficial effects of APP on growth performance and hematological indices of T. putitora.

Dietary ascorbic acid requirement for growth of striped catfish,Pangasianodon hypophthalmus(Sauvage, 1878) juveniles

“…Increased doses of vitamin C in diet increased the ascorbic acid in tissues (Dabrowski, 2000), which was confirmed by liver tissue AA analysis, which showed a similar trend. Further, it confirms the usefulness of optimal inclusion of dietary vitamin C for better growth of P. hypophthalmus juveniles based on the available following findings [Merchie, Lavens, & Sorgeloos, 1997;Phromkunthong, Boonyaratpalin, & Storch, 1997;Ai et al, 2004;Lin & Shiau, 2005;Ai et al, 2006;Eo & Lee, 2008;Nsonga, Kangombe, Mfitilodze, Soko, & Mtethiwa, 2009;Xiao et al, 2010;Khan et al, 2015;Narra et al, 2015;Asaikkutti et al, 2016;Xu et al, 2016].…”

“…Because AA is water soluble, L‐ascorbylpalmitate, L‐ascorbyl sulphate and L‐ascorbyl‐2 polyphosphate generally are used as vitamin C sources in fish feed, but the phosphate derivatives of AA are more effective among all the vitamin C sources (Dabrowski, ; Halver & Hardy, ; NRC, ). Most authors worked in the area of vitamin C nutrition in fish used phosphate derivatives of AA for different fish species, such as Channel catfish, Ictalurus punctatus (Wilson et al., ); Rainbow trout, Oncorhynchus mykiss (Cho & Cowey, ; Matusiewicz, Dabrowski, Volker, & Matusiewicz, ); Tilapia, Oreochromis niloticus (Shiau & Hsu, ; Soliman et al., ); Japanese yellowtail, Seriola quinqueradiata (Kanazawa, Teshima, Koshio, Higashi, & Itoh, ); Mahseer, Tor putitora (Khan et al., ); and Magur, Clarias batrachus (Narra, Rajender, Reddy, Rao, & Begum, ). Therefore, we used L‐ascorbyl‐2 polyphosphate as a AA source in the present study.…”

“…Vitamin C (ascorbic acid) is considered as one of the essential nutrients required for growth and immunity in fish (Asaikkutti, Bhavan, Vimala, Karthik, & Cheruparambath, ; Dabrowski, ; Khan, Zuberi, & Ullah, ; Lim & Lovell, ; NRC, ; Pimpimol, Phoonsamran, & Chitmanat, ; Xu et al., ). Ascorbic acid (AA) has an extensive role in the enhancement of growth, collagen synthesis, iron metabolism, haematology, reproduction, stress physiology, wound healing and immune response in fish (Albrektsen, Lie, & Sandnes, ; Anbarasu & Chandran, ; Halver, Smith, Tolbert, & Baker, ; Roberts, Davies, & Pulsford, ).…”

“…In channel catfish, the utilization of L‐ascorbyl‐2 polyphosphate was higher than that of L‐ascorbyl sulphate (El Naggar & Lovell, ; Wilson et al., ). L‐ascorbyl‐2 polyphosphate is the phosphate derivative, an excellent source and the most stable form (Gadient & Fenster, ; Khan et al., ; Liang, Li, Hou, Qiu, & Zhou, ; Woodward, ), and easily utilized by animals because phosphate groups are readily hydrolysed by digestive enzymes, such as phosphatases (Matusiewicz & Dabrowski, ). In L‐ascorbyl‐2 polyphosphate, the C‐2 carbon atom is esterified and highly reactive, which easily protects vitamin C from oxidation.…”

Effects of dietary selenium nanoparticles on physiological andbiochemical aspects of juvenile Tor putitora

“…The hematological study was carried out according to the method followed by Khan et al (2015). Blood drawn by tail ablation was collected in VACUETTE EDTA K3 tubes for the analysis of hematological indices.…”