Effects of Goussia infecting the blue whiting and phylogenetic placement of Goussia infecting marine fish off Northern Portugal

Xavier, Raquel; Cardoso, João Custódio Fernandes; Barroso, Ricardo; Nogueira, Sara; Cruz, Cristina; Pereira, Ana; Saraiva, Aurélia

doi:10.1007/s00436-020-06727-y

Cited by 5 publications

(1 citation statement)

References 24 publications

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“…in one dog is also very unexpected, given that this is a genus typically associated with parasites of fish and amphibians (Gestal & Azevedo, 2005; Jirků et al., 2009; Molnár et al., 2012). Our ASV had a 100% query cover and identity match with both Goussia balatonica (accession #: GU479659.1) and Goussia chalupskyi (accession #: GU479653.1)—two species associated with fish parasitism (Molnár, 1989; Xavier et al., 2020). Therefore, whether detection of their DNA in dog samples demonstrates biologically viable Goussia cells or another explanation, such as environmental contamination or fish consumption leading to trace quantities of circulating DNA is impossible to conclude (Hornung et al., 2019; Tamburini et al., 2018).…”

Section: Discussionmentioning

confidence: 99%

A multipronged next‐generation sequencing metabarcoding approach unearths hyperdiverse and abundant dog pathogen communities in Cambodia

Huggins

Colella

Koehler

et al. 2021

Transbounding Emerging Dis

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Recent surveys in Southeast Asia, including Cambodia, have identified canine vector‐borne pathogens (VBPs), including those with zoonotic potential, as highly prevalent. The lack of veterinary care alongside the close association semidomesticated dogs have with humans in the region exacerbates these zoonotic risks. Nonetheless, the number of studies investigating such pathogens and the threats they pose to dog and human health is limited. Here, we utilize a next‐generation sequencing (NGS)‐based metabarcoding protocol to conduct an assumption‐free characterization of the bacterial, apicomplexan, and kinetoplastid blood‐borne pathogens of free‐roaming dogs from across Cambodia. From 467 dogs at five field sites, 62% were infected with one of eight confirmed pathogens, comprising Anaplasma platys (32%), Ehrlichia canis (20%), Hepatozoon canis (18%), Babesia vogeli (14%), Mycoplasma haemocanis (13%), the zoonotic pathogen Bartonella clarridgeiae (3%), Candidatus Mycoplasma haematoparvum (0.2%), and Trypanosoma evansi (0.2%). Coinfections of between two and four VBPs were common with 28% of dogs found to have a mixed infection. Moreover, DNA from putatively infectious agents belonging to the bacterial family and genera Coxiella, Mycobacterium, Neisseria, Rickettsiaceae, Treponema, and two uncharacterized Mycoplasma species were identified, in addition to protozoan genera Colpodella, Parabodo, and Bodo. Using a multiple logistic regression model, the presence of ectoparasites, abnormal mucous membranes, anemia, and total protein were found as predictors of canine VBP exposure. This study represents the first time an NGS metabarcoding technique has been used to holistically detect the bacterial and protozoan hemoparasites communities of dogs through an in‐depth survey, highlighting the power of such methods to unearth a wide spectrum of pathogenic organisms in an unbiased manner.

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