Effects of gonadotropin-inhibitory hormone on folliculogenesis and steroidogenesis of cyclic mice

Singh, Padmasana; Krishna, Amitabh; Tsutsui, Kazuyoshi

doi:10.1016/j.fertnstert.2010.03.052

Cited by 63 publications

(47 citation statements)

References 46 publications

(59 reference statements)

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“…The inhibition of testicular activities induced by RFRP3 is not a result of generalized toxicity, as even a higher dose of RFRP3 caused no deleterious effects on the histology of liver and serum AST and ALT activity compared with control mice. The antigonadal function of GnIH/RFRP3 was demonstrated previously in male quail (Ubuka et al , 2014, Syrian Hamster (Zhao et al 2010), and female mice (Singh et al 2011b).…”

Section: Figuresupporting

confidence: 63%

“…Mice in the control group (nZ10) received vehicle only. Based on previous studies, three different doses of RFRP3 (0.02 mg, 0.2 mg, and 2.0 mg/day per body weight; nZ10 per group) dissolved in normal saline solution were selected for the treatment , Singh et al 2011b. The animals were killed by decapitation under a mild dose of anesthetic ether within 30 min after the last injection and blood was collected.…”

Section: In Vivo Studymentioning

confidence: 99%

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Inhibitory roles of the mammalian GnIH ortholog RFRP3 in testicular activities in adult mice

Anjum

Krishna

Tsutsui

2014

Journal of Endocrinology

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The aim of this study was to evaluate the effects of in vivo and in vitro treatments with RFamide-related peptide 3 (RFRP3), a mammalian gonadotropin-inhibitory hormone ortholog, on testicular activities, i.e. spermatogenesis and steroidogenesis, in mice. Mice were treated in vivo with different doses of RFRP3 (control: 0.02 mg, 0.2 mg, and 2.0 mg/day) for 8 days. For in vitro study, the testes of mice were evaluated with different doses of RFRP3 (control: 1 and 10 ng/ml) with or without LH (control: 10 and 100 ng/ml) for 24 h at 37 8C. RFRP3 treatment produced significant changes in the body mass, circulating steroid level, and testicular activity in mice. RFRP3 treatment also caused dose-dependent histological changes in spermatogenesis, such as decline in germ cell proliferation and survival markers and increase in apoptotic markers in testis. Both in vivo and in vitro studies showed the inhibitory effect of RFRP3 on testosterone synthesis in the testis. RFRP3 inhibited the expression of the receptor for LH (LHCGR), STAR protein, cytochrome P450 side-chain cleavage (CYP11A1) and 3b-hydroxysteroid dehydrogenase in the testis, and testosterone secretion dose dependently. This study also suggested that the inhibitory effect of RFRP3 in the testis may be mediated through local production of GnRH. Thus, RFRP3 inhibits testicular steroidogenesis and spermatogenesis either indirectly through GnRH or by directly influencing germ cell proliferation, survival, and apoptosis.

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Section: Figuresupporting

confidence: 63%

Section: In Vivo Studymentioning

confidence: 99%

Inhibitory roles of the mammalian GnIH ortholog RFRP3 in testicular activities in adult mice

Anjum

Krishna

Tsutsui

2014

Journal of Endocrinology

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“…Immunoreactivities of GnRH, GnRH receptor and RFRP-3 were generally found in close vicinity, suggesting functional interaction between GnRH, GnRH-R and RFRP-3 in the mouse ovary (Singh et al, 2011a). Singh et al (2011b) further evaluated the effects of GnIH on the ovarian activity of mice in vivo and in vitro. GnIH (RFRP-3) inhibited follicular development and steroidogenesis in the ovary of mice (Table 1).…”

Section: Pg 22mentioning

confidence: 99%

Molecular, cellular, morphological, physiological and behavioral aspects of gonadotropin-inhibitory hormone

Ubuka

Son

Tsutsui

2016

General and Comparative Endocrinology

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“…In addition to central action of GnIH, direct regulation of gonadal activity by GnIH is becoming clear. GnIH and GnIH receptor are expressed in steroidogenic cells and germ cells in gonads in birds and mammals (Anjum et al, 2014;Bentley et al, 2008;Oishi et al, 2012;Singh et al, 2008Singh et al, , 2011aSingh et al, , 2011bZhao et al, 2010), and GnIH possibly acts by an autocrine/paracrine manner to suppress gonadal steroid production and germ cell differentiation and maturation (Anjum et al, 2014;Bentley et al, 2008;Oishi et al, 2012;Singh et al, 2008Singh et al, , 2011aSingh et al, , 2011bZhao et al, 2010).…”

Section: Direct Regulation Of Gonadal Activity By Gnihmentioning

confidence: 99%

How to contribute to the progress of neuroendocrinology: New insights from discovering novel neuropeptides and neurosteroids regulating pituitary and brain functions

Effects of gonadotropin-inhibitory hormone on folliculogenesis and steroidogenesis of cyclic mice

Cited by 63 publications

References 46 publications

Inhibitory roles of the mammalian GnIH ortholog RFRP3 in testicular activities in adult mice

Inhibitory roles of the mammalian GnIH ortholog RFRP3 in testicular activities in adult mice

Molecular, cellular, morphological, physiological and behavioral aspects of gonadotropin-inhibitory hormone

How to contribute to the progress of neuroendocrinology: New insights from discovering novel neuropeptides and neurosteroids regulating pituitary and brain functions

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