Effects of Glyoxylate, Glycine, and Serine on the Assimilation of 14CO2 in Mesophyll Cells of Chenopodium album

Baumann, G.; Günther, G.

doi:10.1016/s0015-3796(17)30568-1

Cited by 8 publications

(3 citation statements)

References 24 publications

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“…However, glyoxylate decreased the activity of corn bundle sheat cells (Oliver 1978a), of mesophyll cells from Chenopodium sp. (Baumann and Gunther 1979) and of isolated spinach chloroplasts (Oliver and Zelitch 1977).…”

Section: Discussionmentioning

confidence: 99%

Stimulation of nitrogenase activity and photosynthesis in some cyanobacteria by glyoxylate

Bergman

1980

Physiologia Plantarum

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The effect og glyoxylate on nitrogenase activity (C2H2 reduction) and photosynthesis (H14CO3 fixation and O2 evolution) was in vestigated in the three heterocystous cyanobacteria Anabaena cylindrica, A. variabiltis and N. muscorum. Glyoxylate had virtually no effect on the rate of dark respiration and was unable to sustain photoheterotrophic growth, though some slight stimulation (= 30%) of photorophic growth was noted. A considerable stimulation of both nitrogenase and photosynthetic activities was observed in presence of glyoxylate. In the light the stimulation increased with time up to about 15‐25 h after adding optimal concentrations of 4–6 mM glyoxylate. Placing glyoxylate treated samples in the dark or adding DCMU (30 μM) in the light, showed that glyoxylate initially supported significantly higher nitrogenase activity than did samples in absence of glyoxylate. However, after a prolonged incubation in the dark or in presence of DCMU glyoxylate is unable to relieve the adverse effects of such conditions. The stimulation of the nitrogenase activity was even more pronounced when the glyoxylate was added to cells preincubated in the dark (“carbon starved”) than for cells kept constantly in light. The results suggest that glyoxylate, or a metabolite, may act as an inhibitor of cyanobacterial photorespiration and this hypothesis is discussed.

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Section: Discussionmentioning

confidence: 99%

Stimulation of nitrogenase activity and photosynthesis in some cyanobacteria by glyoxylate

Bergman

1980

Physiologia Plantarum

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“…The elevated CO2 concentrations would also decrease glycolate synthesis and therefore photorespir-ation by the cells. The nonfacilitated nature of CO2 uptake into the chloroplast (22) (2) were able to show that the glyoxylate treatment strongly inhibited glycine and serine synthesis in isolated Chenopodium album cells. They were not, however, able to see any increase in net photosynthesis under the conditions employed.…”

Section: Methodsmentioning

confidence: 99%

“…Thirty s later the reaction was terminated by adding 0.1 ml 3 N HCI. The reaction flasks contained 50 pl of a 4:1 (v/v) ethanol to toluene solution, 0.35 mM RuBP, the indicated amount of NaH'4CO3, (2)(3)(4)uCi/,umol) …”

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confidence: 99%

The Effect of Glyoxylate on Photosynthesis and Photorespiration by Isolated Soybean Mesophyll Cells

Oliver

1980

Plant Physiol.

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Incubating isolated soybean leaf mesophyO ceOs with glyoxylate increased the rates of CO2 fixation by as much as 150%. In order to cause this stimulation, the glyoxylate must be presented to the cels before the NaHCO. (13,20). Photosynthesis was assayed in stoppered 10-ml Fernbach flasks in a reaction mixture that contained 0.3 M sorbitol, 50 mm Tris-HCl (pH 7.8), 2 mm NaNO3, 2 mm EDTA, 1 mm MnCl2, 1 mM MgCl2, 0.5 mm K2HPO4, and 2 mm DTT (added fresh daily).The isolated soybean leaf cells, containing 5-30 jig Chl and suspended in the same solution, were generally added to previously equilibrated flasks in a 25 C waterbath and illuminated for 15 s to 20 min in the absence of added NaHCO3. Next the NaH'4CO3 was added and the illumination continued for an additional 5 min before the reaction was stopped by adding 0.1 ml 3 N HCI. The amount of acid-stable radioactivity was determined by liquid scintillation counting of 0.1-or 0.2-ml aliquots of the acidified reaction mixture.All reactions were in equilibrium with air unless otherwise indicated. In some experiments the reaction vessels were equilibrated with C02-free gases by blowing the gas vigorously against the liquid surface for 10 min before the cells were added. The activity of the RuBP carboxylase in the cells was assayed by first making the membrane more permeable with toluene (7) and then adding the carboxylation substrates RuBP and NaH14CO3. The cells were preilluminated and assayed in the same solution that was used in the photosynthetic studies except that the MgC12 concentration was increased to 25 mm (1). After the cells were illuminated at 25 C for 5 min, 25-,lI samples of the cell mixture were transferred to the reaction flasks. Thirty s later the reaction was terminated by adding 0.1 ml 3 N HCI. The reaction flasks contained 50 pl of a 4:1 (v/v) ethanol to toluene solution, 0.35 mM RuBP, the indicated amount of NaH'4CO3, (2)(3)(4)uCi/,umol)

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Regulation of Photorespiration

Chaguturu¹,

Chollet

1981

Commentaries in Plant Science

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Effects of Glyoxylate, Glycine, and Serine on the Assimilation of 14CO2 in Mesophyll Cells of Chenopodium album

Cited by 8 publications

References 24 publications

Stimulation of nitrogenase activity and photosynthesis in some cyanobacteria by glyoxylate

Stimulation of nitrogenase activity and photosynthesis in some cyanobacteria by glyoxylate

The Effect of Glyoxylate on Photosynthesis and Photorespiration by Isolated Soybean Mesophyll Cells

Regulation of Photorespiration

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