Effects of glucose metabolism pathways on sperm motility and oxidative status during long-term liquid storage of goat semen

Qiu, Jianhua; Li, You-Wei; Xie, Hong-Li; Li, Qing; Dong, Haibo; Sun, Mingwei; Gao, Wei‐Qiang; Tan, Jing-He

doi:10.1016/j.theriogenology.2016.03.005

Cited by 34 publications

(24 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, Qiu et al (2016) observed an increase in ROS only when goat semen was cooled for more than three days, justifying our findings of low cellular stress in buck semen cooled for a shorter period at 5°C.…”

Section: Discussionsupporting

confidence: 88%

Can centrifugation force compromise the plasmatic membrane, acrosome and DNA integrity of goat spermatozoa?

Crespilho¹,

Bosco²,

Dell’Aqua³

et al. 2018

Braz. J. Vet. Res. Anim. Sci.

View full text Add to dashboard Cite

Protocols for cooling or freezing goat semen usually recommend centrifugation for seminal plasma removal. However, little is known about the effect of this process on goat sperm viability and functionality. The present study evaluated the effects of centrifugation force on the plasma membrane, acrosomes, and DNA integrity of goat semen. Four ejaculates from each of the four different Anglo Nubian male goats were used. Semen samples were obtained using artificial vagina, and immediately after collection, ejaculates were diluted using Ringer’s sodium lactate solution and split into three groups: Control (CG, without centrifugation), G1 (centrifugation 600 x g/10 min), G2 (centrifugation 1200 x g/10 min). After centrifugation, seminal plasma was removed, the sperm pellets were resuspended using Tris-egg yolk extender (80 x 106 spermatozoa/mL) and the sperm morphology was analyzed. Samples were cooled at 5°C for 5, 24, 36, and 48 h and then sperm plasma membrane and acrosome integrity (PMAI, %) and sperm DNA fragmentation index (SDF, %) were evaluated at each time-point, using a flow cytometer. Additionally, sperm movement was determined using computer semen analysis (CASA) after 5, 24, and 48 h of refrigeration period. The semen centrifugation did not induce additional sperm morphology defect or reduction in sperm kinetics in the experimental groups. Differences were not observed (p > 0.05) in PMAI and SDF among different groups, in any of each timepointof the cooling process. In conclusion, centrifugation, even at high speeds, did not affect goat sperm integrity and functionality when submitted to refrigeration process.

show abstract

Section: Discussionsupporting

confidence: 88%

Can centrifugation force compromise the plasmatic membrane, acrosome and DNA integrity of goat spermatozoa?

Crespilho¹,

Bosco²,

Dell’Aqua³

et al. 2018

Braz. J. Vet. Res. Anim. Sci.

View full text Add to dashboard Cite

show abstract

“…Although physiological amounts of ROS are linked to normal sperm function, spermatozoa are highly vulnerable to oxidative stress caused by the accumulation of ROS due to exhaustion of their antioxidant reserves and deterioration of sperm morphology and functions (Zhao et al, ). According to Plessis et al (), both pathways of energy metabolism were essential for sperm function and successful fertilization, while sperm equilibrium between the pathways is controlled by the external factors (such as medium composition, oxygen levels and extracellular pH), the metabolic phenotype of the species, and the functional status of spermatozoa (Qiu et al, ). This suggested optimization of conditions may influence spermatozoa metabolism manner and have a beneficial effect on the long‐term preservation of boar spermatozoa in vitro.…”

Section: Introductionmentioning

confidence: 99%

Protective influence of rosiglitazone against time‐dependent deterioration of boar spermatozoa preserved at 17°C

Wang

Yang

Guo

et al. 2019

Reprod Domestic Animals

View full text Add to dashboard Cite

Spermatozoa are highly specialized cells, and energy metabolism plays an important role in modulating sperm viability and function. Rosiglitazone is an antidiabetic drug in the thiazolidinedione class that regulates metabolic flexibility and glucose uptake in various cell types, but its effects on boar sperm metabolism are unknown. In this study, we investigated the potential effect of rosiglitazone against time‐dependent deterioration of boar spermatozoa during liquid preservation at 17°C. Freshly ejaculated semen was diluted with Beltsville Thawing Solution (BTS) containing different concentrations of rosiglitazone, and the motility, membrane and acrosome integrity of sperm were detected. Besides, we measured glucose uptake capacity, l‐lactate production level, mitochondrial membrane potential, adenosine triphosphate (ATP) content and mitochondrial reactive oxygen species (mROS) production of sperm after boar semen had been incubated with or without rosiglitazone, iodoacetate (glycolysis inhibitor) and rotenone (electron transport chain inhibitor) for 5 days. The addition of rosiglitazone significantly enhanced sperm quality and had a strong protective effect on the sperm membrane and acrosome integrity during storage. BTS containing 50 μM rosiglitazone maintained the total motility of liquid‐preserved sperm above 60% for 7 days. Rosiglitazone improved sperm quality by regulating energy metabolism manner of preserved sperm, protected the sperm mitochondrial membrane potential, enhanced sperm ATP production and in the meanwhile reduced mROS through enhancing glycolysis but not oxidative phosphorylation. The data suggested the practical feasibility of using rosiglitazone for improving boar spermatozoa quality during semen preservation.

show abstract

“…With respect to galactose metabolism, which plays an established role in energy delivery [62]. A considerable number of in vitro experiments has revealed that energy substrates, such as glucose and fructose added to diluents can prolong sperm viability and activity [63][64][65]. This indicated that high energy metabolic and antioxidant defense activities in the SST cells may be two of the factors responsible for long-term sperm retention or maintenance in the female RT.…”

Section: Discussionmentioning

confidence: 99%

Detrimental effects of excessive fatty acid secretion on female sperm storage in chickens

Wen

Mai

Wang

et al. 2020

J Animal Sci Biotechnol

View full text Add to dashboard Cite

Background: Female sperm storage (FSS), the maintenance of sperm inside the female reproductive tract for an extended period of time, is pervasive among organisms with internal fertilization. Because FSS enables asynchronous mating and fertilization, it could be extremely important to reproduction. However, the physiological mechanisms underlying prolonged preservation and maintenance are poorly understood. Here, we used chicken, a typical oviparous animal, to determine the mechanisms ensuring sperm functionality in sperm storage tubules (SSTs). Results: We performed an insemination experiment on over two thousand hens at two periods, and found that the FSS capabilities varied widely among individuals. Except for the differences in the SST density between the two groups with distinct FSS abilities, we quantitatively profiled small-molecule metabolites derived from SST cells, and identified 28 metabolites with differential expression. In particular, high levels of lipids, fatty acids and lipid peroxidation product were observed in hens with low FSS capability. Pathway analysis showed that these differential metabolites were significantly enriched in the biosynthesis of unsaturated fatty acids. Moreover, we detected the total antioxidant capacity and lipid peroxidation level of SSTs, and found that chickens with a lower FSS ability had a significantly higher content of lipid peroxidation end-product, which was 2.4-fold greater than chickens with a higher FSS capability, and no significant difference was found in the total antioxidant capacity between these two groups. Conclusions: Our findings reveal that the long-term storage of sperm and the maintenance of their function in the female reproductive tract require an adequate microenvironment. The superabundance of fatty acids secreted by SST cells had detrimental effects on sperm storage in the female reproductive tract. Lipid peroxidation produces toxic biological substances that may cause irreversible damage to resident spermatozoa, resulting in short-term sperm retention and decreased fertility. Our findings provide new avenues for studying sperm storage and sustaining fertility.

show abstract

Effects of glucose metabolism pathways on sperm motility and oxidative status during long-term liquid storage of goat semen

Cited by 34 publications

References 46 publications

Can centrifugation force compromise the plasmatic membrane, acrosome and DNA integrity of goat spermatozoa?

Can centrifugation force compromise the plasmatic membrane, acrosome and DNA integrity of goat spermatozoa?

Protective influence of rosiglitazone against time‐dependent deterioration of boar spermatozoa preserved at 17°C

Detrimental effects of excessive fatty acid secretion on female sperm storage in chickens

Contact Info

Product

Resources

About