Effects of glucose concentration in semen extender and storage temperature on stallion sperm quality following long-term cooled storage

Hernández‐Avilés, Camilo; Love, Charles C.; Serafini, R.; Ramírez‐Agámez, Luisa; Friedrich, Macy; Ghosh, S. N.; Teague, S.R.; LaCaze, K.A.; Brinsko, Steven P.; Varner, D.D.

doi:10.1016/j.theriogenology.2020.02.007

Cited by 18 publications

(11 citation statements)

References 46 publications

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“…Nevertheless, these two molecules can be used as the substitute for another one. Addition of glucose in extenders combined with hypothermia might exert a synergetic effect on long-term preservation in the liquid state [ 120 , 215 ].…”

Section: Semen Extenders Used In Non-freezing Storage Of Spermatozoamentioning

confidence: 99%

Extend the Survival of Human Sperm In Vitro in Non-Freezing Conditions: Damage Mechanisms, Preservation Technologies, and Clinical Applications

Cheng

Jiang

et al. 2022

Cells

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Preservation of human spermatozoa in vitro at normothermia or hypothermia maintaining their functions and fertility for several days plays a significant role in reproductive biology and medicine. However, it is well known that human spermatozoa left in vitro deteriorate over time irreversibly as the consequence of various stresses such as the change of osmolarity, energy deficiency, and oxidative damage, leading to substantial limitations including the need for semen examinations, fertility preservation, and assisted reproductive technology. These problems may be addressed with the aid of non-freezing storage techniques. The main and most effective preservation strategies are the partial or total replacement of seminal plasma with culture medium, named as extenders, and temperature-induced metabolic restriction. Semen extenders consist of buffers, osmolytes, and antioxidants, etc. to protect spermatozoa against the above-mentioned adverse factors. Extended preservation of human spermatozoa in vitro has a negative effect on sperm parameters, whereas its effect on ART outcomes remains inconsistent. The storage duration, temperature, and pre-treatment of semen should be determined according to the aims of preservation. Advanced techniques such as nanotechnology and omics have been introduced and show great potential in the lifespan extension of human sperm. It is certain that more patients will benefit from it in the near future. This review provided an overview of the current knowledge and prospects of prolonged non-freezing storage of human sperm in vitro.

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Section: Semen Extenders Used In Non-freezing Storage Of Spermatozoamentioning

confidence: 99%

Extend the Survival of Human Sperm In Vitro in Non-Freezing Conditions: Damage Mechanisms, Preservation Technologies, and Clinical Applications

Cheng

Jiang

et al. 2022

Cells

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“…The globally accepted cooling temperature is between 4°C and 5°C to maintain semen quality and reduce gamete metabolism in dogs [16], stallions [17], bulls [18], goats [19], and rams [20], whereas in boars, the diluted liquid semen must be kept between 15°C and 17°C [21]. Mammalian sperm cells, especially for rams [22], boars [23], bulls [24], stallions [12], and men [25], are damaged by rapid temperature drop. The temperature of post-chilled semen may not be as harmful to the intactness of the male sperm as the post-thawed technique, which results in low spermatozoal value [26].…”

Section: Cooling Temperature For Semen Preservationmentioning

confidence: 99%

“…Gent ® A (Minitüb GmbH, Tiefenbach, Germany) is a commercial extender containing egg yolk and is used for the preservation of semen for a long time [178]. BotuSemen ® is a commercial extender that contains a skim milk base used for preserving frozen sperm of stallions [12]. EquiPlus ® is a commercial extender that contains defined milk proteins used to preserve semen [26].…”

Section: Commercial Extendersmentioning

confidence: 99%

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Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders

Bustani

Baiee

2021

Vet World

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Reproduction is fundamental for all living things as it ensures the continued existence of a species and an improved economy in animal husbandry. Reproduction has developed since history, and diverse processes, such as artificial insemination and in vitro fertilization, have been developed. Semen extenders were discovered and developed to protect sperm from harmful factors, such as freeze and osmotic shock, oxidative stress, and cell injury by ice crystals. Semen extenders preserve sperm by stabilizing its properties, including sperm morphology, motility, and viability and membrane, acrosomal, and DNA integrity. Therefore, semen extenders must provide a favorable pH, adenosine triphosphate, anti-cooling and anti-freeze shock, and antioxidant activity to improve semen quality for fertilization. Hence, this review provides precise data on different semen extenders, preservative mechanisms, and essential additives for semen extenders in different animals.

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“…Usually, diluents are comprised of components such as sugars, electrolytes, buffers, egg yolk, milk, and milk products [ 6 ]. Sugars such as glucose enhance sperm motility during semen storage [ 7 ]. A proper ratio of electrolytes and nonelectrolytes in the extender is important for maintaining the optimal osmotic pressure and the buffering agents in extenders play a key role in prolonging the sperm motility.…”

Section: Introductionmentioning

confidence: 99%

The efficiency of different types of extenders for semen cooling in stallions

Rečková

Filipčík

Soušková³

et al. 2022

Anim Biosci

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Objective: This study was focused on the influence of the skimmed milk-based extender (SM), the INRA 96 extender and BotuSemen Gold extender on parameters of stallions' ejaculate during storage.Methods: In this study, 14 stallions between 4 and 20 years of age were monitored. Total andTotal and pProgressive motility, viability and morphology of sperm were evaluated at time intervals of 24, 48 and 72 hours after collection. Results:The total and progressive motility was significantly higher in the INRA 96 and BotuSemen Gold extenders than in the skimmed milk-based extender at all time intervals (p < 0.05). After 48 and 72 hours, the INRA 96 preserved the viability of the semen better than the SM (p < 0.01). After 72 hours, the share of viable spermatozoa in the INRA 96 samples was 59.16 ± 1.06% and in the BotuSemen Gold samples, 55.00 ± 1.20% (p < 0.05) The total motility, progressive motility and values of sperm with normal morphology were significantly higher in the INRA 96 and BotuSemen Gold extenders than in the skimmed milk-based extender (p < 0.01). The sperm viability differed significantly in all extenders (p < 0.01). The highest value of sperm viability was in INRA 96 (64.69 ± 0.67%) and lowest in skimmed milk-based extender (59.70 ± 0.81%). The highest differences occurred at 72 hours of storage. The highest differences occurred at 72 hours of storage.Values of total motility, progressive motility and sperm viability decreased over time (p < 0.01). In case of sperm morphology there was no statistically significant decrease between 48-and 72-hour time intervals. Conclusion:It can be concluded that the extenders with a chemically defined composition have shown better indicators of insemination capabilities in ejaculates than the skimmed milkbased extender. The BotuSemen Gold extender is a suitable alternative to the INRA 96, when

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Effects of glucose concentration in semen extender and storage temperature on stallion sperm quality following long-term cooled storage

Cited by 18 publications

References 46 publications

Extend the Survival of Human Sperm In Vitro in Non-Freezing Conditions: Damage Mechanisms, Preservation Technologies, and Clinical Applications

Extend the Survival of Human Sperm In Vitro in Non-Freezing Conditions: Damage Mechanisms, Preservation Technologies, and Clinical Applications

Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders

The efficiency of different types of extenders for semen cooling in stallions

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