Effects of gluconate on biomass improvement and light stress tolerance of Haematococcus pluvialis in mixotrophic culture

Pang, Na; Gu, Xiangyu; Xiao, Fu; Chen, Shulin

doi:10.1016/j.algal.2019.101647

Cited by 21 publications

(15 citation statements)

References 39 publications

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“…2). Since astaxanthin is lipid-soluble dispersing in lipid droplets in H. pluvialis cells (15,16,39), it is not surprising that lipid and astaxanthin syntheses are closely associated, and conditions favor lipid synthesis also enhance astaxanthin production (40)(41)(42).…”

Section: Discussionmentioning

confidence: 99%

Enhancing Algal Biomass, Lipid and Astaxanthin Production by Mix-Cultivation of Haematococcus Pluvialis With Simplicillium Lanosoniveum DT06

Yan

Xing

Dong

et al. 2021

Preprint

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To enhance algal lipid and astaxanthin synthesis, the astaxanthin-producing green alga Haematococcus pluvialis was mix-cultivated with the antibiotic-synthesizing fungus Simplicillium lanosoniveum DT06 under non-aseptic conditions (NM) in this study. Results showed that in contrast to aseptic pure culture (AH) and non-aseptic pure culture (NH) of H. pluvialis , the production of cell (biomass), lipids and astaxanthin increased 56% and 119%, 112.4% and 279%, 74% and 175%, reaching 2.45 g/l, 0.837 g/l and 88.84 mg/l respectively; the average growth rate and the average specific growth rate increased 60.8% and 133.1 %, 19% and 31.6%, reaching 194.2 mg l -1 d -1 and 0.25 d -1 respectively; and the average lipid synthesis rate and average specific lipid synthesis rate increased 112.5% and 278.66%, 36.15% and 97%, reaching 69.75 mg l -1 d -1 and 28.47 mg g -1 d -1 respectively; and also the content of C16-C18 fatty acids that are suitable for biofuels production increased to 83.19%. Therefore, NM provides an efficient and economical way for the production of biofuels.

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Section: Discussionmentioning

confidence: 99%

Enhancing Algal Biomass, Lipid and Astaxanthin Production by Mix-Cultivation of Haematococcus Pluvialis With Simplicillium Lanosoniveum DT06

Yan

Xing

Dong

et al. 2021

Preprint

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“…The increase of CO 2 level up to 20% would lead to chloroplast inhibition and decreasing cell growth, resulting in high cell mortality [ 39 , 41 ]. Multiple studies have recently reported on a maximum cell number of H. pluvialis in the region of 2.43 × 10 6 cells/mL after providing it with sodium gluconate at 2 g L −1 and illumination intensity at 105 ± 3 μmol m −2 s −1 [ 42 ].…”

Section: Current Production Strategy To Induce Biomass At the Greementioning

confidence: 99%

“…Illumination is also a significant factor for improving the biomass productivity for H. pluvialis in mixotrophic culture [ 42 ]. Radiation energy in the life cycle of H. pluvialis contributes to the conversion of antenna pigments to chemical energy in the form of ATP and NADPH via a photosynthetic electron transport chain.…”

Section: Current Production Strategy To Induce Biomass At the Greementioning

confidence: 99%

A Review on Haematococcus pluvialis Bioprocess Optimization of Green and Red Stage Culture Conditions for the Production of Natural Astaxanthin

et al. 2021

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As the most recognizable natural secondary carotenoid astaxanthin producer, the green microalga Haematococcus pluvialis cultivation is performed via a two-stage process. The first is dedicated to biomass accumulation under growth-favoring conditions (green stage), and the second stage is for astaxanthin evolution under various stress conditions (red stage). This mini-review discusses the further improvement made on astaxanthin production by providing an overview of recent works on H. pluvialis, including the valuable ideas for bioprocess optimization on cell growth, and the current stress-exerting strategies for astaxanthin pigment production. The effects of nutrient constituents, especially nitrogen and carbon sources, and illumination intensity are emphasized during the green stage. On the other hand, the significance of the nitrogen depletion strategy and other exogenous factors comprising salinity, illumination, and temperature are considered for the astaxanthin inducement during the red stage. In short, any factor that interferes with the cellular processes that limit the growth or photosynthesis in the green stage could trigger the encystment process and astaxanthin formation during the red stage. This review provides an insight regarding the parameters involved in bioprocess optimization for high-value astaxanthin biosynthesis from H. pluvialis.

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“…Laraib et al obtained 137.43 ± 13.3 mg/l/d of biomass productivity and 39% of lipid content during the mixotrophic cultivation of Chlorella vulgaris when molasses utilized as additional carbon source, respectively 1.5-fold and 2-fold higher than that of photoautotrophic cultivation ( Laraib et al, 2021 ). Pang et al introduced sodium gluconate using as an unconventional organic carbon source for the mixotrophic cultivation of Haematococcus pluvialis ( Pang et al, 2019b ). Yang et al utilized sodium acetate to counter the growth inhibition of Chlamydomonas reinhardtii by nutrient deficiency, the lipid production increased by 93% under same nutrient deficiency condition ( Yang et al, 2018 ).…”

Section: Optimization Of Nutrient Conditionsmentioning

confidence: 99%

Enhancing microalgal lipid accumulation for biofuel production

Zhu

Sun²,

Fei³

et al. 2022

Front. Microbiol.

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Microalgae have high lipid accumulation capacity, high growth rate and high photosynthetic efficiency which are considered as one of the most promising alternative sustainable feedstocks for producing lipid-based biofuels. However, commercialization feasibility of microalgal biofuel production is still conditioned to the high production cost. Enhancement of lipid accumulation in microalgae play a significant role in boosting the economics of biofuel production based on microalgal lipid. The major challenge of enhancing microalgal lipid accumulation lies in overcoming the trade-off between microalgal cell growth and lipid accumulation. Substantial approaches including genetic modifications of microalgal strains by metabolic engineering and process regulations of microalgae cultivation by integrating multiple optimization strategies widely applied in industrial microbiology have been investigated. In the present review, we critically discuss recent trends in the application of multiple molecular strategies to construct high performance microalgal strains by metabolic engineering and synergistic strategies of process optimization and stress operation to enhance microalgal lipid accumulation for biofuel production. Additionally, this review aims to emphasize the opportunities and challenges regarding scaled application of the strategic integration and its viability to make microalgal biofuel production a commercial reality in the near future.

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Effects of gluconate on biomass improvement and light stress tolerance of Haematococcus pluvialis in mixotrophic culture

Cited by 21 publications

References 39 publications

Enhancing Algal Biomass, Lipid and Astaxanthin Production by Mix-Cultivation of Haematococcus Pluvialis With Simplicillium Lanosoniveum DT06

Enhancing Algal Biomass, Lipid and Astaxanthin Production by Mix-Cultivation of Haematococcus Pluvialis With Simplicillium Lanosoniveum DT06

A Review on Haematococcus pluvialis Bioprocess Optimization of Green and Red Stage Culture Conditions for the Production of Natural Astaxanthin

Enhancing microalgal lipid accumulation for biofuel production

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