Effects of Ghrelin on intestinal cell proliferation, the expression of protein absorption and metabolism factors in juvenile grass carp (Ctenopharyngodon idella)

Zhou, Yonghua; Guo, Meixing; Li, Yingjie; Xu, Wenqian; Zhao, Dongxin; Chen, Yuan; Zuo, Anli; Qu, Fufa; Tang, Jianzhou; Cao, Shenping; Liu, Zhen

doi:10.1016/j.aqrep.2021.100928

Cited by 7 publications

(7 citation statements)

References 26 publications

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“…Bighead carp is a typical lter-feeding sh and one of the stomach-less sh, so it mostly relies on the gut's absorption and digestion processes to obtain nutrients. According to recent studies, ghrl promotes intestinal cell proliferation and in uences the expression of functional genes related to protein expression and metabolism [21,37]. The intestinal expression of ghrl was much higher than those in other tissues based on the results of ghrl differential expression and ISH, which indicated that ghrl gene may be strongly associated with the feeding, digestion, and absorption functions of bighead carp.…”

Section: Discussionmentioning

confidence: 98%

Molecular cloning and expression characteristics of ghrl and ghsr genes in bighead carp (Hypophthalmichthys nobilis)

Wang

Luo

et al. 2023

Preprint

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Backgroud Ghrelin (ghrl) and its receptor gene, ghsr (growth hormone secretagogue receptor), regulate numerous physiological functions, including growth hormone secretion and food intake. In the present study, we investigated the sequences and expression characteristics of the two genes in bighead carp (Hypophthalmichthys nobilis). Methods Cloning and expression characteristics of the ghrl and ghsr gene were got and analyzed by using the RACE technique, qRT-PCR, and in situ hybridization. The sizes of the experimental fish were 1.21 ± 0.20 kg in winter (6–7 months old) and 2.34 ± 0.20 kg in summer (18–19 months old). Results The ghrl and ghsr genes, encoded 106 and 346 amino acids, respectively, and were 1208 bp and 2239 bp in length. Early blastocysts had the highest levels of ghrl and ghsr expression throughout early development (P < 0.01). The gut exhibited the highest levels of expression of ghrl, whereas significantly higher levels of ghsr (P < 0.01) were detected in the brain. Significantly higher levels of expression of ghrl and ghsr were detected in the intestine and muscle in summer, when compared with the levels in winter (P < 0.05). Based on in situ hybridization, intestine ghrl expression signals were more pronounced than intestinal ghsr expression signals, whereas ghsr expression signals were higher than those of ghrl in brain tissues. The co-expression patterns of related genes in the growth hormone synthesis, secretion and action pathway (ko04935), and cAMP signaling pathway (ko04024) also indicated that ghrl and ghsr are involved in the feeding regulation of bighead fish. Conclusion Our findings suggest that ghrl and ghsr potentially play roles in bighead carp feeding and associated energy metabolism activities, in addition to growth performance.

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Section: Discussionmentioning

confidence: 98%

Molecular cloning and expression characteristics of ghrl and ghsr genes in bighead carp (Hypophthalmichthys nobilis)

Wang

Luo

et al. 2023

Preprint

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“…The intestine is an important site for digestion, absorption and nutrient exchange of crustaceans, and it is also one of the most closely related parts between organism and the external environment. PEPT1 transported large amounts of amino acids in dipeptides/tripeptides form ( Terova et al., 2009 ; Xu et al., 2020 ; Zhou et al., 2022 ). Small peptides are more efficiently absorbed than free amino acids, so PEPT1 has great potential to optimize absorption ( Wang et al., 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Replacement of fish meal with cottonseed protein concentrate in Chinese mitten crab (Eriocheir sinensis): Nutrient digestibility, growth performance, free amino acid profile, and expression of genes related to nutrient metabolism

Liu,

Zou,

Wang

et al. 2024

Animal Nutrition

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“…Furthermore, a functional correlation between PepT1 and some GI hormones, such as ghrelin, has been demonstrated in zebrafish [83] and grass carp (Ctenopharyngodon idella) [84]. Several authors suggest that intestinal nutrient transport mechanisms involving the intestinal peptide transporter PEPT1 are involved in the control of food intake in a high-protein diet through the involvement of the enteroendocrine system.…”

Section: Discussionmentioning

confidence: 99%

Effects of Bioactive Peptides from Atlantic Salmon Processing By-Products on Oxyntopeptic and Enteroendocrine Cells of the Gastric Mucosa of European Seabass and Gilthead Seabream

Clavenzani,

Lattanzio,

Bonaldo

et al. 2023

Animals

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The present study was designed to evaluate the effects of dietary levels of bioactive peptides (BPs) derived from salmon processing by-products on the presence and distribution of peptic cells (oxyntopeptic cells, OPs) and enteric endocrine cells (EECs) that contain GHR, NPY and SOM in the gastric mucosa of European seabass and gilthead seabream. In this study, 27 seabass and 27 seabreams were divided into three experimental groups: a control group (CTR) fed a control diet and two groups fed different levels of BP to replace fishmeal: 5% BP (BP5%) and 10% BP (BP10%). The stomach of each fish was sampled and processed for immunohistochemistry. Some SOM, NPY and GHR-IR cells exhibited alternating “open type” and “closed type” EECs morphologies. The BP10% group (16.8 ± 7.5) showed an increase in the number of NPY-IR cells compared to CTR (CTR 8.5 ± 4.8) and BP5% (BP10% vs. CTR p ≤ 0.01; BP10% vs. BP5% p ≤ 0.05) in the seabream gastric mucosa. In addition, in seabream gastric tissue, SOM-IR cells in the BP 10% diet (16.8 ± 3.5) were different from those in CTR (12.5 ± 5) (CTR vs. BP 10% p ≤ 0.05) and BP 5% (12.9 ± 2.5) (BP 5% vs. BP 10% p ≤ 0.01). EEC SOM-IR cells increased at 10% BP (5.3 ± 0.7) compared to 5% BP (4.4 ± 0.8) (5% BP vs. 10% BP p ≤ 0.05) in seabass. The results obtained may provide a good basis for a better understanding of the potential of salmon BPs as feed ingredients for seabass and seabream.

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Effects of Ghrelin on intestinal cell proliferation, the expression of protein absorption and metabolism factors in juvenile grass carp (Ctenopharyngodon idella)

Cited by 7 publications

References 26 publications

Molecular cloning and expression characteristics of ghrl and ghsr genes in bighead carp (Hypophthalmichthys nobilis)

Molecular cloning and expression characteristics of ghrl and ghsr genes in bighead carp (Hypophthalmichthys nobilis)

Replacement of fish meal with cottonseed protein concentrate in Chinese mitten crab (Eriocheir sinensis): Nutrient digestibility, growth performance, free amino acid profile, and expression of genes related to nutrient metabolism

Effects of Bioactive Peptides from Atlantic Salmon Processing By-Products on Oxyntopeptic and Enteroendocrine Cells of the Gastric Mucosa of European Seabass and Gilthead Seabream

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