Effects of ghrelin on developmental competence and gene expression of in vitro fertilized ovine embryos

Wang, Zhengguang; Lin, Ping; Yu, Shuai

doi:10.1016/j.theriogenology.2012.11.026

Cited by 27 publications

(28 citation statements)

References 47 publications

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“…Another study found greater blastocyst-formation rate and hatching in oocytes treated with ghrelin (Dovolou et al, 2014b). This positive effect of ghrelin on animal fertility was demonstrated in other farm animals as well in a different range of concentrations spectrum (Young et al, 2001;Zhang et al, 2007;Dashtizad et al, 2010;Wang et al, 2013). These studies, combined with our results suggest that reduced ghrelin levels during the summer lead to impaired oocytes and embryos development and quality, and elevated ghrelin levels might contribute to the improved fertility in dairy cows during the winter.…”

Section: Discussionsupporting

confidence: 82%

“…In addition to appetite control regulation and GH secretion, ghrelin takes part in many other physiological processes, including stress (Chuang et al, 2011;Diz-Chaves, 2011), sleep and memory (Steiger et al, 2011), regulating insulin secretion (Broglio et al, 2001) and glucose uptake (Gershon and Vale, 2014 Another major physiological process affected by ghrelin is fertility (Rak-Mardyla, 2013). Previous studies in many farm animals demonstrated that ghrelin actions on reproduction performances are within a concentration spectrum (Young et al, 2001;Zhang et al, 2007;Du et al, 2010;Dashtizad et al, 2010;Wang et al, 2013). However, less is known of ghrelin and seasonal effect in the cow, especially the dairy cow, in which a major fertility issue has emerged in conjunction with selection for increased milk production.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Seasonal and parity effects on ghrelin levels throughout the estrous cycle in dairy cows

Honig

Ofer

Elbaz

et al. 2016

General and Comparative Endocrinology

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Seasonal and parity effects on ghrelin levels throughout the estrous cycle in dairy cows

Honig

Ofer

Elbaz

et al. 2016

General and Comparative Endocrinology

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“…It has been also reported that high concentrations of ghrelin (250 ng/ml) decrease the cleavage rate of sheep oocytes and deteriorate blastocyst quality (Wang et al. ). Dashtizad et al.…”

Section: Discussionmentioning

confidence: 99%

Ghrelin Accelerates In Vitro Maturation of Bovine Oocytes

Dovolou

Messinis

Periquesta

et al. 2014

Reprod Domestic Animals

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Ghrelin, apart from its metabolic role, is nowadays considered as a basic regulator of reproductive functions of mammals, acting at central and gonadal levels. Here, we investigated for possible direct actions of ghrelin on in vitro maturation of bovine oocytes and for its effects on blastocyst yield and quality. In experiment 1, cumulus oocyte complexes (COCs) were matured in the presence of four different concentrations of ghrelin (0, 200, 800 and 2000 pg/ml). In vitro fertilization and embryo culture were carried out in the absence of ghrelin, and blastocyst formation rates were examined on days 7, 8 and 9. In experiment 2, only the 800 pg/ml dose of ghrelin was used. Four groups of COCs were matured for 18 or 24 h (C18, Ghr18, C24 and Ghr24), and subsequently, they were examined for oocyte nuclear maturation and cumulus layer expansion; blastocysts were produced as in experiment 1. The relative mRNA abundance of various genes related to metabolism, oxidation, developmental competence and apoptosis was examined in snap-frozen cumulus cells, oocytes and day-7 blastocysts. In experiment 1, ghrelin significantly suppressed blastocyst formation rates. In experiment 2, more ghrelin-treated oocytes matured for 18 h reached MII compared with controls, while no difference was observed when maturation lasted for 24 h. At 18 and 24 h, the cumulus layer was more expanded in ghrelin-treated COCs than in the controls. The blastocyst formation rate was higher in Ghr18 (27.7 ± 2.4%) compared with Ghr24 (17.5 ± 2.4%). Differences were detected in various genes' expression, indicating that in the presence of ghrelin, incubation of COCs for 24 h caused over-maturation (induced ageing) of oocytes, but formed blastocysts had a higher hatching rate compared with the controls. We infer that ghrelin exerts a specific and direct role on the oocyte, accelerating its maturational process.

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“…A study of the in vitro effects of different doses of ghrelin on ovine oocyte maturation and embryo development has been recently published (Wang et al 2013). While the smaller doses of ghrelin (50 ng/ml) increased blastocyst rates and the total number of cells per blastocyst (compared with 0 or 10 ng/ml), a higher dose (250 ng/ml) decreased these parameters, suggesting that there is an 'appropriate' concentration of the peptide that promotes blastocyst formation (Wang et al 2013). This is consistent with the results obtained in our study, in which, the addition of not only ghrelin but also the antagonist altered embryo progress.…”

Section: Parametersmentioning

confidence: 99%

Role of ghrelin in fertilization, early embryo development, and implantation periods

Luque¹,

Torres²,

Loredo³

et al. 2014

Reproduction

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In order to clarify the physiological role of ghrelin in gestation, we evaluated the effects of administration of exogenous ghrelin (2 or 4 nmol/animal per day) or its antagonist (6 nmol/animal per day of (D-Lys 3 )GHRP6) on fertilization, early embryo development, and implantation periods in mice. Three experiments were performed, treating female mice with ghrelin or its antagonist: i) starting from 1 week before copulation to 12 h after copulation, mice were killed at day 18 of gestation; ii) since ovulation induction until 80 h later, when we retrieved the embryos from oviducts/uterus, and iii) starting from days 3 to 7 of gestation (peri-implantation), mice were killed at day 18. In experiments 1 and 3, the antagonist and/or the highest dose of ghrelin significantly increased the percentage of atrophied fetuses and that of females exhibiting this finding or a higher amount of corpora lutea compared with fetuses (nCL/nF) (experiment 3: higher nCL/nF-atrophied fetuses: ghrelin 4, 71.4-71.4% and antagonist, 75.0-62.5% vs ghrelin 2, 46.2K15.4% and control, 10-0.0%; nZ7-13 females/group; P!0.01). In experiment 2, the antagonist diminished the fertilization rate, and both, ghrelin and the antagonist, delayed embryo development (blastocysts: ghrelin 2, 62.5%; ghrelin 4, 50.6%; and antagonist, 61.0% vs control 78.4%; nZ82-102 embryos/treatment; P!0.0001). In experiment 3, additionally, ghrelin (4 nmol/day) and the antagonist significantly diminished the weight gain of fetuses and dams during pregnancy. Our results indicate that not only hyperghrelinemia but also the inhibition of the endogenous ghrelin effects exerts negative effects on the fertilization, implantation, and embryo/fetal development periods, supporting the hypothesis that ghrelin (in 'adequate' concentrations) has a physiological role in early gestational events.

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Effects of ghrelin on developmental competence and gene expression of in vitro fertilized ovine embryos

Cited by 27 publications

References 47 publications

Seasonal and parity effects on ghrelin levels throughout the estrous cycle in dairy cows

Seasonal and parity effects on ghrelin levels throughout the estrous cycle in dairy cows

Ghrelin Accelerates In Vitro Maturation of Bovine Oocytes

Role of ghrelin in fertilization, early embryo development, and implantation periods

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