Effects of genetically modified Burkholderia pyrrocinia JK‐SH007E1 on soil microbial community in poplar rhizosphere

Abstract

Summary It is important to assess the biosafety of genetically engineered bacteria before release into the environment. In a previous study, the genetically engineered bacterium Burkholderia pyrrocinia JK‐SH007E1 was developed, showing biological control activity against a poplar canker disease. In the present work, the potential effects of B. pyrrocinia JK‐SH007E1 on soil microbial diversity and population structure in the poplar rhizosphere were assessed. The micro‐ecological effects of JK‐SH007E1 on soil microbial communities were investigated using substrate utilization assays (Biolog EcoPlate), enzyme activity assays and 16S rRNA sequencing to research species diversity and population structure of soil microbial communities. The genetically engineered isolate JK‐SH007E1 improved the functional diversity, soil enzyme activities and alpha diversity of soil microbial communities. However, the positive effects after inoculation attenuated with time, except for soil enzyme activities. Treatments with JK‐SH007 or JK‐SH007E1 were not different in impacts on soil enzyme activity and functional diversity of soil microbial communities. The results demonstrated that inoculation of JK‐SH007E1 into the poplar rhizosphere posed little or no threat to soil microbial communities in the long term. This work provides an additional insight into the effects of genetically engineered JK‐SH007E1 on the environment during long‐term use as biological control against poplar canker diseases.

Physiological Diversity of Spitsbergen Soil Microbial Communities Suggests Their Potential as Plant Growth-Promoting Bacteria

“…Our research did not prove the strong dependence between pH of soil [ 25 ] and DH activity ( Figure 5 ). Increase in DH activity (LN-3, REIN-1, CAL-4) may represent and stimulate soil microbial activity and number ( Figure 1 A), and as a result increase the quantities and qualities of nutrients available to plants, demonstrated in the elevation of AWCD and catabolic diversity presented by NUSE, H, or R indicators ( Figure 4 , Figure 5 B, Figure 8 C), which was also observed by He et al [ 38 ].…”

Horizon scanning of potential environmental applications of terrestrial animals, fish, algae and microorganisms produced by genetic modification, including the use of new genomic techniques

“…Biocontrol applications include a number of developments in bacteria, e.g., Burkholderia pyrrocinia ( He et al, 2018 ) and fungi, e.g., Beauveria bassiana , Metarhizium acridum , Trichoderma harzianum, Isaria fumosorosea ( Kim et al, 2013 ; Hu and Wu, 2016 ; Xia et al, 2018 ; Tong et al, 2021 ; Asgari, 2023 ), which are modified to enhance the efficacy of known biocontrol agents to control different pests or pathogens. Some of these applications express RNAs which trigger a RNAi-based response in the exposed target pests to achieve a biocontrol effect.…”