Effects of genetic variants for the swine FABP3, HMGA1, MC4R, IGF2, and FABP4 genes on fatty acid composition

Hong, Joon-Ki; Kim, Du-Wan; Cho, Kyuho; Sa, Soo-Jin; Choi, Sunho; Kim, Young-Hwa; Park, Jun-Cheol; Schmidt, G. S.; Davis, Michael; Chung, Hoyoung

doi:10.1016/j.meatsci.2015.06.011

Cited by 15 publications

(7 citation statements)

References 40 publications

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“…Finally, in order to complete the set of genes, we chose genes which have been described in the literature to play different roles (fatty acid synthesis, transport, storage and oxidation) in muscle lipid metabolism, prioritizing those genes which are expressed in muscle, such as transcriptional factors, co-factors and nuclear receptors ( ETS1, LPIN1, NR1H3, NCOA1, NCOA6, PPARA, PPARD, PRKAA1, RXRG, SP1, SREBF1 ) 54 (freely available at http://www.bioguo.org/AnimalTFDB/ ), enzymes ( ACSS1, ACSS2, CPT1B, CROT, DGAT1, DGAT2 , PDHX ), as well as the IGF2 gene, which has been described as the causal factor of the imprinted QTL for muscle growth and fat deposition in a Meishan × Large White intercross 27 ( Supplementary Table S6 ). Moreover, polymorphisms in the IGF2 gene have been significantly associated with muscle FA composition in swine 55 .…”

Section: Methodsmentioning

confidence: 99%

Expression-based GWAS identifies variants, gene interactions and key regulators affecting intramuscular fatty acid content and composition in porcine meat

Puig-Oliveras

Revilla

Castelló

et al. 2016

Sci Rep

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The aim of this work is to better understand the genetic mechanisms determining two complex traits affecting porcine meat quality: intramuscular fat (IMF) content and its fatty acid (FA) composition. With this purpose, expression Genome-Wide Association Study (eGWAS) of 45 lipid-related genes associated with meat quality traits in swine muscle (Longissimus dorsi) of 114 Iberian × Landrace backcross animals was performed. The eGWAS identified 241 SNPs associated with 11 genes: ACSM5, CROT, FABP3, FOS, HIF1AN, IGF2, MGLL, NCOA1, PIK3R1, PLA2G12A and PPARA. Three expression Quantitative Trait Loci (eQTLs) for IGF2, ACSM5 and MGLL were identified, showing cis-acting effects, whereas 16 eQTLs had trans regulatory effects. A polymorphism in the ACSM5 promoter region associated with its expression was identified. In addition, strong candidate genes regulating ACSM5, FOS, PPARA, PIK3R1, PLA2G12A and HIF1AN gene expression were also seen. Notably, the analysis highlighted the NR3C1 transcription factor as a strong candidate gene involved in the regulation of the 45 genes analysed. Finally, the IGF2, MGLL, MC2R, ARHGAP6, and NR3C1 genes were identified as potential regulators co-localizing within QTLs for fatness and growth traits in the IBMAP population. The results obtained increase our knowledge in the functional regulatory mechanisms involved in these complex traits.

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Section: Methodsmentioning

confidence: 99%

Expression-based GWAS identifies variants, gene interactions and key regulators affecting intramuscular fatty acid content and composition in porcine meat

Puig-Oliveras

Revilla

Castelló

et al. 2016

Sci Rep

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“…On the other hand, oleic acid is the most abundant MUFA in pork, comprising nearly 35%-45% of total FAs content. It is associated with consumer’s acceptability of high quality cured products, in terms of organoleptic, technological and nutritional values of meat [41]. To study the effect of IGF2 : g .…”

Section: Resultsmentioning

confidence: 99%

Analysis of porcine IGF2 gene expression in adipose tissue and its effect on fatty acid composition

et al. 2019

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IGF2 : g . 3072G>A polymorphism has been described as the causal mutation of a maternally imprinted QTL for muscle growth and fat deposition in pigs. The objective of the current work was to study the association between the IGF2 : g . 3072G>A polymorphism and the IGF2 gene expression and its effect on fatty acid composition in adipose tissue in different pig genetic backgrounds. A cis -eQTL region associated with the IGF2 mRNA expression in adipose tissue was identified in an eGWAS with 355 animals. The IGF2 gene was located in this genomic interval and IGF2g . 3072G>A was the most significant SNP, explaining a 25% of the gene expression variance. Significant associations between IGF2 : g . 3072G>A polymorphism and oleic (C18:1(n-9); p-value = 4.18x10 -07 ), hexadecanoic (C16:1(n-9); p-value = 4.04x10 -07 ), linoleic (C18:2(n-6); p-value = 6.44x10 -09 ), α-linoleic (C18:3(n-3); p-value = 3.30x10 -06 ), arachidonic (C20:4(n-6); p-value = 9.82x10 -08 ) FAs and the MUFA/PUFA ratio (p-value = 2.51x10 -9 ) measured in backfat were identified. Animals carrying the A allele showed an increase in IGF2 gene expression and higher PUFA and lower MUFA content. However, in additional studies was observed that there could be other proximal genetic variants affecting FA composition in adipose tissue. Finally, no differences in the IGF2 gene expression in adipose tissue were found between heterozygous animals classified according to the IGF2 : g . 3072G>A allele inherited from the father ( A P G M or A M G P ). However, pyrosequencing analysis revealed that there is imprinting of the IGF2 gene in muscle and adipose tissues, with stronger differences among the paternally and maternally inherited alleles in muscle. Our results suggested that IGF2 : g . 3072G>A polymorphism plays an important role in the regulation of IGF2 gene expression and can be involved in the fatty acid composition in adipose tissue. In both cases, further studies are still needed to deepen the mechanism of regulation of IGF2 gene expression in adipose tissue and the IGF2 role in FA composition.

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“…The FABP3 gene is associated with fat content in the carcass, intramuscular fat and meat quality, and its main functions are the regulation of fatty acid uptake and intracellular transport (Chmurzyńska, 2006;Hong et al, 2015). Adipose tissue may influence reproductive traits because it plays an endocrine role, such as the metabolism of sex hormones (Pinto, 2014).…”

Section: Fabp3 (Snpg-240t>c)mentioning

confidence: 99%

Research Article Association of MC4R, FABP3 and DGAT1 gene polymorphisms with reproductive traits in two domestic pig lines

Gondim¹,

Soares²,

Lugo³

et al. 2019

Genet. Mol. Res.

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MC4R, FABP3 and DGAT1 are important genes related to productive and reproductive traits in pigs, such as food intake, growth rate, back fat thickness reduction and lactation. Selection of pigs for lean meat production may lead to losses in reproductive charcters because reduction of backfat thickness and low feed intake during the lactation phase can affect reproduction. To examine this possibility, we evaluated possible associations of SNPs in the MC4R (SNPg.1,578C>T), FABP3 (SNPg.240T>C) and DGAT1 (SNPg.9,422C>T) genes with the number of weaned piglets, birth weight, total weight of litter at birth, total litter weight at weaning, age at first mating and parity interval in 227 animals of two maternal lines: European and European/Chinese pigs. Specific fragments of the genes under study were amplified by Multiplex ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 18 (3): gmr18139 V.S. Gondim et al. 2 ARMS-PCR and genotyped in an automatic sequencer. SNPs in the MC4R (SNPg.1,578C>T) and FABP3 (SNPg-240T>C) genes showed three genotypic variations in both lines, while the SNP (SNPg.9,422C>T) of the DGAT1 gene had three genotypic variations in European swine and two genotypic variations (homozygotes) in the European/Chinese breed. Polymorphisms of MC4R, FABP3 and DGAT1 genes evaluated by variance analysis had significant associations with the reproductive traits age at first mating and parity interval, while only the DGAT1 gene in the European line was significantly associated with the production traits mean weight at weaning and total litter weight at birth andnd weaning.

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Effects of genetic variants for the swine FABP3, HMGA1, MC4R, IGF2, and FABP4 genes on fatty acid composition

Cited by 15 publications

References 40 publications

Expression-based GWAS identifies variants, gene interactions and key regulators affecting intramuscular fatty acid content and composition in porcine meat

Expression-based GWAS identifies variants, gene interactions and key regulators affecting intramuscular fatty acid content and composition in porcine meat

Analysis of porcine IGF2 gene expression in adipose tissue and its effect on fatty acid composition

Research Article Association of MC4R, FABP3 and DGAT1 gene polymorphisms with reproductive traits in two domestic pig lines

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