Effects of genetic changes to the begomovirus/betasatellite complex causing cotton leaf curl disease in South Asia post-resistance breaking

Briddon, R. W.; Akbar, Fazal; Iqbal, Zafar; Amrao, Luqman; Amin, Imran; Saeed, Muhammad; Mansoor, Shahid

doi:10.1016/j.virusres.2013.12.008

Cited by 51 publications

(36 citation statements)

References 52 publications

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“…The CLCuKoV-Bu strain, with a truncated TrAP, is the only begomovirus strain associated with the resistance breaking of CLCuD currently found throughout Pakistan, with the occasional identification of other strains (Table S3) 9, 43 . We have found that some recent isolates of CLCuKoV-Bu encode a full length TrAP (Hassan et al ., unpublished data).…”

Section: Discussionmentioning

confidence: 99%

“…The CLCuKoV-Bu which is the dominant strain associated with CLCuD from 2000 onward in cultivated cotton, encodes a truncated C2 protein of 35 amino acids (aa) 9, 14 . TrAP encoded by begomoviruses is a multi-functional protein, functioning as a pathogenicity determinant 44 and also a strong suppressor of post transcriptional gene silencing activity (PTGS) of the host 45 .…”

Section: Discussionmentioning

confidence: 99%

“…CLCuD was associated with several distinct monopartite begomoviruses – Cotton Leaf curl Multan virus (CLCuMuV), Cotton leaf curl Kokhran virus (CLCuKoV), Cotton leaf curl Alabad virus (CLCuAV) and Papaya leaf curl virus (PaLCuV) 4, 6, 12 , and a single betasatellite (Cotton leaf curl Multan betasatellite [CLCuMuB]) 13, 14 . Following the appearance of the second CLCuD epidemic in cotton, to present time, a predominant single recombinant begomovirus named as Cotton leaf curl Kokhran virus - Burewala (CLCuKoV-Bu), previously known as Cotton leaf curl Burewala virus (CLCuBuV), was associated with CLCuD in Pakistan and India 9 .…”

Section: Introductionmentioning

confidence: 99%

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Multiple begomoviruses found associated with cotton leaf curl disease in Pakistan in early 1990 are back in cultivated cotton

Zubair

Zaidi

Shakir

et al. 2017

Sci Rep

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118

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The first epidemic of cotton leaf curl disease (CLCuD) in early 1990’s in the Indian subcontinent was associated with several distinct begomoviruses along with a disease-specific betasatellite. Resistant cotton varieties were introduced in late 1990’s but soon resistance was broken and was associated with a single recombinant begomovirus named Burewala strain of Cotton leaf curl Kokhran virus that lacks a full complement of a gene encoding a transcription activator protein (TrAP). In order to understand the ongoing changes in CLCuD complex in Pakistan, CLCuD affected plants from cotton fields at Vehari were collected. Illumina sequencing was used to assess the diversity of CLCuD complex. At least three distinct begomoviruses characterized from the first epidemic; Cotton leaf curl Multan virus, Cotton leaf curl Kokhran virus and Cotton leaf curl Alabad virus, several distinct species of alphasatellites and cotton leaf curl Multan betasatellite were found associated with CLCuD. These viruses were also cloned and sequenced through Sanger sequencing to confirm the identity of the begomoviruses and that all clones possessed a full complement of the TrAP gene. A new strain of betasatellite was identified here and named CLCuMuBVeh. The implications of these findings in efforts to control CLCuD are discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Multiple begomoviruses found associated with cotton leaf curl disease in Pakistan in early 1990 are back in cultivated cotton

Zubair

Zaidi

Shakir

et al. 2017

Sci Rep

Self Cite

118

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“…Begomoviruses in the Old World are mostly associated with symptom/pathogenicity determinant betasatellites and self-replicating alphasatellites (Zhou, 2013). Betasatellites encode βC1 protein and play critical role in important diseases like cotton leaf curl disease in the Indian Subcontinent (Briddon et al, 2014). …”

Section: Introductionmentioning

confidence: 99%

Engineering Plant Immunity: Using CRISPR/Cas9 to Generate Virus Resistance

et al. 2016

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Plant viruses infect many economically important crops, including wheat, cotton, maize, cassava, and other vegetables. These viruses pose a serious threat to agriculture worldwide, as decreases in cropland area per capita may cause production to fall short of that required to feed the increasing world population. Under these circumstances, conventional strategies can fail to control rapidly evolving and emerging plant viruses. Genome-engineering strategies have recently emerged as promising tools to introduce desirable traits in many eukaryotic species, including plants. Among these genome engineering technologies, the CRISPR (clustered regularly interspaced palindromic repeats)/CRISPR-associated 9 (CRISPR/Cas9) system has received special interest because of its simplicity, efficiency, and reproducibility. Recent studies have used CRISPR/Cas9 to engineer virus resistance in plants, either by directly targeting and cleaving the viral genome, or by modifying the host plant genome to introduce viral immunity. Here, we briefly describe the biology of the CRISPR/Cas9 system and plant viruses, and how different genome engineering technologies have been used to target these viruses. We further describe the main findings from recent studies of CRISPR/Cas9-mediated viral interference and discuss how these findings can be applied to improve global agriculture. We conclude by pinpointing the gaps in our knowledge and the outstanding questions regarding CRISPR/Cas9-mediated viral immunity.

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“…This was the start of the second epidemic of CLCuD in this region and here referred as “Burewala epidemic” (Sattar et al, 2013). It was speculated that dispense of C2 gene in CLCuKoV-Bu was a key player in resistance breaking (Amrao et al, 2010b; Briddon et al, 2014). However, the recent reports contradict this speculation as new field isolates of CLCuKoV-Bu with intact C2 gene have been reported from India (Kumar et al, 2015; Godara et al, 2016).…”

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confidence: 99%

The Prediction of a New CLCuD Epidemic in the Old World

Sattar

Iqbal

Tahir³

et al. 2017

Front. Microbiol.

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Cotton leaf curl disease (CLCuD), the most complex disease of cotton, is a major limiting biotic factor to worldwide cotton productivity. Several whitefly-transmitted monopartite begomoviruses causing CLCuD have been characterized and designated as CLCuD-associated begomoviruses. Despite of being reported over 100 years ago in Africa, CLCuD became economically pandemic causing massive losses to cotton production in Pakistan and India during past couple of decades. In Asia, cotton has faced two major epidemics during this period viz. “Multan epidemic” and “Burewala epidemic.” The “Multan epidemic” era was 1988–1999 after which the virus remained calm until 2002 when “Burewala epidemic” broke into the cotton fields in Indo-Pak subcontinent, till 2013–2014. However, both the epidemics were caused by monopartite begomovirus complex. Similarly in Africa, Cotton leaf curl Gezira virus with associated DNA-satellites causes CLCuD. Quite recently, in the Old World (both Asia and Africa), bipartite begomoviruses have started appearing in the areas under cotton cultivation. Under such aggravated circumstances, it seems we are heading toward another epidemic of CLCuD in the Old World. Here we articulate the causes and potential emergence of the third epidemic of CLCuD in Asia. The current situation of CLCuD in Asia and Africa is also discussed.

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Effects of genetic changes to the begomovirus/betasatellite complex causing cotton leaf curl disease in South Asia post-resistance breaking

Cited by 51 publications

References 52 publications

Multiple begomoviruses found associated with cotton leaf curl disease in Pakistan in early 1990 are back in cultivated cotton

Multiple begomoviruses found associated with cotton leaf curl disease in Pakistan in early 1990 are back in cultivated cotton

Engineering Plant Immunity: Using CRISPR/Cas9 to Generate Virus Resistance

The Prediction of a New CLCuD Epidemic in the Old World

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