Effects of gastric pH on the activity of exogenous pentosanase and the effect of pentosanase supplementation of the diet on the performance of growing-finishing pigs

Thacker, P. A.; Baas, T. C.

doi:10.1016/s0377-8401(96)01028-0

Cited by 17 publications

(14 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The hypothesis that the ability of wheat phytases to improve the degradation of wheat phytate is superior to that of microbial phytases was tested at different pH values. The values chosen for this experiment (pH 3–5.5) are in line with the range of pH in digesta of pigs35–39 and broilers 40–43. It is sometimes argued that phytases need to have high optimal activity at low pH (2–3) as they are assumed to act in the upper gastrointestinal tract of pigs7–10 and broilers 11, 12.…”

Section: Resultsmentioning

confidence: 89%

“…It is sometimes argued that phytases need to have high optimal activity at low pH (2–3) as they are assumed to act in the upper gastrointestinal tract of pigs7–10 and broilers 11, 12. However, it has been shown that pH in the stomach of pigs increases after feed ingestion35, 36, 39 and thus feed enzymes are probably emptied out of the stomach before pH returns to very low pre‐prandial values. Stomach pH ranged from 3.8 to 4.6 when pigs had free access to feed 37, 38.…”

Section: Resultsmentioning

confidence: 99%

“…Stomach pH ranged from 3.8 to 4.6 when pigs had free access to feed 37, 38. When feed was offered as a meal, the reported peak stomach pH values range from 4.7 to 5.6 35, 36, 39. and, 2 h after feeding, stomach pH ranged from 2.6 to 4.8 35, 36, 39.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

The degradation of phytate by microbial and wheat phytases is dependent on the phytate matrix and the phytase origin

Brejnholt

Dionisio

Glitsoe

et al. 2011

J. Sci. Food Agric.

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

The degradation of phytate by microbial and wheat phytases is dependent on the phytate matrix and the phytase origin

Brejnholt

Dionisio

Glitsoe

et al. 2011

J. Sci. Food Agric.

View full text Add to dashboard Cite

show abstract

“…Xylanases are also extensively used in animal feed in an attempt to improve feed digestion and the performance of poultry and pigs [10][11][12][13]. Recently, much research about xylanase has been focused on alkaline xynlanase for the use in pulp industry [2,14].…”

Section: Introductionmentioning

confidence: 99%

Improvement of Aspergillus sulphureus Endo-β-1,4-Xylanase Expression in Pichia pastoris by Codon Optimization and Analysis of the Enzymic Characterization

Zhang

Chen

et al. 2009

Appl Biochem Biotechnol

View full text Add to dashboard Cite

The gene xynB from Aspergillus sulphureus encoding the endo-beta-1,4-xylanase was de novo synthesized by splicing overlap extension polymerase chain reaction according to Pichia pastoris protein's codon bias. The synthetic DNA and wild-type DNA were placed under the control of a glyceraldehyde-3-phosphate dehydrogenase gene promoter (GAP) in the constitutive expression vector plasmid pGAPzalphaA and electrotransformed into the P. pastoris X-33 strain, respectively. The transformants screened by Zeocin were able to constitutively secrete the xylanase in YPD liquid medium. The maximum yield of the recombinant xylanase produced by the synthetic DNA was 105 U ml(-1), which was about 5-fold higher than that by wild-type DNA under the flask culture at 28 degrees Celsius for 3 days. The enzyme showed optimal activity at 50 degrees Celsius and pH 5.0. The residual activity remained above 90% after the recombinant xylanase was pretreated in Na(2)HPO(4)-citric acid buffer (pH 2.4) for 2 h. The xylanase activity was significantly improved by Zn(2+). These biochemical characteristics suggest that the recombinant xylanase has a prospective application in feed industry as an additive.

show abstract

“…Enzyme supplementation has also been shown to improve the performance of pigs fed rye based diets (Thacker et al, 1991;Thacker and Baas, 1996) indicating that pentosans may also be a problem for swine. The degree of improvement is not as great as that seen with poultry and tends to be greater when diets were fed in a mash form rather than as a pellet (Thacker et al, 1991) Recently a breeding program has been undertaken at the University of Saskatchewan to reduce the viscosity of rye varieties which may extend the use of rye as a feed grain with or without enzyme supplementation.…”

Section: Introductionmentioning

confidence: 99%

Performance of young growing pigs (17-34 kg) fed rye-based diets selected for reduced viscosity

Thacker¹,

Campbell²,

Scoles³

1999

J. Anim. Feed Sci.

View full text Add to dashboard Cite

Forty eight crossbred pigs (17.0 kg) were assigned to one of three dietary treatments in a 3 x 2 factorial design experiment. The control diet was based on barley and soyabean meal while the two experimental diets contained 60% of either high or low viscosity rye. Pigs fed either of the rye-based diets had significantly higher (P<0.05) digestibility coefficients for dry matter and gross energy than did pigs fed barley. The digestibility coefficients for dry matter, crude protein and gross energy were numerically higher for pigs fed the low viscosity rye than the high viscosity rye (P>0.05). Pigs fed either of the rye-based diets consumed significantly less feed and gained weight at a slower rate than did pigs fed the barley-based diet. The pigs fed the low viscosity rye gained 8.7% faster and consumed 9% more feed than pigs fed the high viscosity diet but these differences did not reach statistical significance (P>0.05). The overall results indicate that selection to reduce the viscosity of rye only slightly improved the nutritive value of rye for pigs. The magnitude of the improvement indicates that a substantial reduction in viscosity is necessary in order to observe a measurable difference in pig performance.

show abstract

Effects of gastric pH on the activity of exogenous pentosanase and the effect of pentosanase supplementation of the diet on the performance of growing-finishing pigs

Cited by 17 publications

References 15 publications

The degradation of phytate by microbial and wheat phytases is dependent on the phytate matrix and the phytase origin

The degradation of phytate by microbial and wheat phytases is dependent on the phytate matrix and the phytase origin

Improvement of Aspergillus sulphureus Endo-β-1,4-Xylanase Expression in Pichia pastoris by Codon Optimization and Analysis of the Enzymic Characterization

Performance of young growing pigs (17-34 kg) fed rye-based diets selected for reduced viscosity

Contact Info

Product

Resources

About