Effects of Gastric and Jejunal Juice Extracts on Gastric Acid Secretion

Abstract

SEMB, L. S. Effects of gastric and jejunal juice extracts on gastric acid secretion.Acta physiol. scand. 1969. 77. 385-395. The specificity of inhibition of i.v. injected extracts prepared from the gastric antral juice of dogs was studied by comparison with extracts prepared from canine fundic and jejunal pouches. The extracts were prepared by dialysis against water and lyophilization, and injected in doses of 1 mg/kg b.w. into Heidenhain pouch dogs secreting at a steady state from constantly infused histamine. Extracts of antral juice (22 expts. in 5 dogs) were found to inhibit gastric secretion in 17 expts. (average 63.6 %), while in 4 expts. there was stimulation of gastric secretion (average 57 %). Extracts of fundic juice were tested in 21 expts. in 4 dogs, and showed inhibition in 10 (average 50.4 %) and stimulation in 10 expts. (average 54.8 %). The extracts obtained from jejunal secretions were tested in 15 expts. on 3 dogs, and showed inhibition of gastric acid secretion in 9 expts. (average 50.6 %), while in 4 expts. there was significant stimulation of the secretion (47.7 %). Rectal temperature was increased more than 0.5" C in 8 of the experiments with antral juice extracts, in 4 with fundic extracts, and in 7 with jejunal extracts. Inhibition or stimulation was observed in these experiments. I t is concluded that dialyzed and lyophilized extracts from gastrointestinal secretions contain substances that either stimulate or inhibit gastric secretion. The inhibitory effect could not be completely explained by pyrogenicity of extracts.

The Effect of Gastric Juice Extracts on Histamine as a Gastric Secretory Stimulant

“…The dialyzed and lyophilized extracts of the secretions from both the fundic and the antral pouches were prepared as detailed earlier (Semb 1969).…”

“…However, the mode of action of this "gastric inhibitory substance" (GIS) is not clear. Previous studies have shown that intravenous injection of extracts from gastrointestinal secretions inhibit acid responses to histamine (Semb 1969). I n preliminary tests, S.C. or i.m.…”

“…The doses of the extracts from antrum or fundus were also of the same order of magnitude as those shown previously to give inhibition upon i.v. injection (Semb 1966a(Semb , 1969. I n earlier studies, it was shown that inhibition of gastric acid secretion took place about 3/4-1 hr after the i.v.…”