Effects of Garlic Extract (allicin) on Proliferation of Endothelial Progenitor Cells (EPC) in Patients with Stable Coronary Artery Disease

Putri, Alisia Yuana; Pikir, Budi Susetyo; Oktaviono, Yudi Her; Alzahra, Fatimah

doi:10.1088/1755-1315/441/1/012163

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…Allicin has been shown to have an antioxidant effect and have scavenging ability. 18 Allicin with its antioxidant capability has been shown able to increase the availability of eNOS, NO, and trigger growth factor such VEGF, SDF-1 and IGF production-which are required for EPCs proliferation, 11,19 Increasing level of NO also increase the level of Vasodilator-stimulated phosphoprotein (VASP) which plays role in the actin elongation which improves EPCs migration. 20,21 Allicin also act as H 2 S donor which can interact with Cys1045 dan Cys1024 Mitogen-Activated Protein Kinase (MAPK) activation.…”

Section: Discussionmentioning

confidence: 99%

High Dose Allicin with Vitamin C Improves EPCs Migration from the Patient with Coronary Artery Disease

Oktaviono¹,

Amadis²,

Al-Farabi³

2020

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MATERIAL AND METHODS Allicin and Vitamin C preparation Garlic extract contains Allicin (ChemFaces ®) 98% was dissolved in dimethyl sulphoxide (DMSO). Vitamin C powder (Sigma Aldrich, USA) was dissolved in double-distilled water and diluted with culture medium to acquire the dose of 250 mcg/mL. Sample criteria The blood sample was acquired from eight CAD patients in Dr Soetomo General Hospital who fulfilled the inclusion criteria, which are: male, aged ABSTRACT Endothelial Progenitor Cells (EPCs) have an important role in endothelial dysfunction repairment through neovasculogenesis and cardiac myocytes regeneration. However, EPCs migration is greatly reduced in the patient with Coronary Artery Disease (CAD). Allicin and Vitamin C are hypothesized to improve EPCs migration due to its antioxidant properties. Objective: To investigate the effect of Allicin and its combination with Vitamin C in EPCs migration of CAD patients. Material and Method: Mononuclear cells were isolated from CAD patients and cultured on fibronectin-coated plates with colony-forming unit Hill medium. The cells were divided into untreated (control), Allicin treatment (dose 100 mcg/ml, 200 mcg/ml, 400 mcg/ ml), and each dose of Allicin combined with 250 mcg/mL of Vitamin C. EPCs migration was assessed with Transwell Migration Assay Kit and evaluated by using statistical tests. Results: This research shows that EPC migration was significantly higher in the treatment. Allicin at all dose (dose 100 mcg/ml, 200 mcg/ml, 400 mcg/ml) and its combination with 250 mcg/mL of vitamin C compared to untreated group (p<0.05). Allicin increase EPCs migration in a dosedependent manner. However, the only combination of 400 mcg/ml Allicin with 250 mcg/mL of vitamin C which has significantly higher EPCs migration compared to Allicin treatment alone. Conclusion: Allicin improves EPCs migration in a dose-dependent manner. Improvement of the migration only observed on the Allicin dose 400 mcg/ml with Vitamin C.

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Section: Discussionmentioning

confidence: 99%

High Dose Allicin with Vitamin C Improves EPCs Migration from the Patient with Coronary Artery Disease

Oktaviono¹,

Amadis²,

Al-Farabi³

2020

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The effect of <i>Dayak</i> onion brewed water in reducing blood pressure and mean arterial pressure (MAP) in hypertensive patients

Setiani,

Shoufiah,

Widiastuti

et al. 2023

Healthc Low-resour S

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Background: The World Health Organization (WHO) estimated that 1.28 billion adults aged 30-79 years worldwide suffered from hypertension. Dayak onion is an herbal plant found in Indonesia, particularly in Kalimantan and traditionally used to treat hypertension. The purpose of this study was to determine the effect of Dayak onion steeping water on the reduction of systolic-diastolic blood pressure and mean arterial pressure (MAP) in hypertensive patients. Methods: This study employed a quasi-experimental research design with a time series approach, utilizing a pre-post-test design with a control group. The sample included two groups (intervention and control), totaling 30 participants. The independent variable was the steeping of Dayak onion bulbs, while the dependent variables were blood pressure values and MAP. The instruments used were Standard Operating Procedures (SPO) Dayak onion herb, SPO blood pressure measurement, SPO calculation of MAP, and a digital sphygmomanometer. Data analysis was performed using paired t-tests. Results: The results of the paired t-test statistical analysis of systolic-diastolic values and MAP in each group revealed significant findings. In the intervention group, a significant result was obtained in the pre-post test difference test, with a p-value of < 0.05 from day 1 to day 3 assessments. This suggests that Dayak onion steeping water had an effect on systolic-diastolic and MAP values in the intervention group. In contrast, the pre-post test in the control group yielded a p-value of < 0.05 for systolic values on days 2 and 3, diastolic values on days 1 and 3, and MAP values on days 1, 2, and 3. This indicates differences in systolic-diastolic and MAP values in the pre-post assessments, although these differences were not evenly distributed across every day. Conclusion: The study found that Dayak onion steeping water had an effect on systolic-diastolic and MAP values in the intervention group. Thus, the use of Dayak onion steeping water, containing allicin, can be considered an approach for controlling hypertension in the realm of complementary and alternative medicine, utilizing natural ingredients for herbal therapy.

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Effects of Garlic Extract (allicin) on Proliferation of Endothelial Progenitor Cells (EPC) in Patients with Stable Coronary Artery Disease

Cited by 2 publications

References 11 publications

High Dose Allicin with Vitamin C Improves EPCs Migration from the Patient with Coronary Artery Disease

High Dose Allicin with Vitamin C Improves EPCs Migration from the Patient with Coronary Artery Disease

The effect of <i>Dayak</i> onion brewed water in reducing blood pressure and mean arterial pressure (MAP) in hypertensive patients

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