Effects of Garlic Compounds Diallyl Sulfide and Diallyl Disulfide on Arylamine N-Acetyltransferase Activity in Strains of<i>Helicobacter pylori</i>from Peptic Ulcer Patients

Chung, Jing Gung; Chen, G. W.; Wu, Lii Tzu; Chang, Huei Ling; Lin, J.; Yeh, Chun-Chieh; Wang, T. F.

doi:10.1142/s0192415x98000397

Cited by 57 publications

(29 citation statements)

References 34 publications

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“…The bactericidal activity of these sulfides correlates with the number of sulfur atoms contained within them (73). It has also been indicated that the bactericidal effects of DAS and DADS on H. pylori, K. pneumoniae, and P. aeruginosa were due to their activity with respect to inhibition of arylamine N-acetyltransferase, an enzyme found in these pathogens (18,19,37,53,73). In another study, a 30-min exposure of C. albicans to DADS (0.5 mM) induced 70% cell death within 2 h. The early intracellular events associated with DADS-induced cell death corresponded to increased oxidative stress mediated through decreased GSH levels, resulting in the drastic death of C. albicans organisms (51).…”

Section: Discussionmentioning

confidence: 99%

In Vitro Efficacy of Diallyl Sulfides against the Periodontopathogen Aggregatibacter actinomycetemcomitans

Velliyagounder

Ganeshnarayan

Velusamy

et al. 2012

Antimicrob Agents Chemother

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The in vitro antibacterial effects of diallyl sulfide (DAS) against the Gram-negative periodontopathogen Aggregatibacter actinomycetemcomitans, the key etiologic agent of the severe form of localized aggressive periodontitis and other nonoral infections, were studied. A. actinomycetemcomitans was treated with garlic extract, allicin, or DAS, and the anti-A. actinomycetemcomitans effects of the treatment were evaluated. Garlic extract, allicin, and DAS significantly inhibited the growth of A. actinomycetemcomitans (greater than 3 log; P < 0.01) compared to control cells. Heat inactivation of the garlic extracts significantly reduced the protein concentration; however, the antimicrobial effect was retained. Purified proteins from garlic extract did not exhibit antimicrobial activity. Allicin lost all its antimicrobial effect when it was subjected to heat treatment, whereas DAS demonstrated an antimicrobial effect similar to that of the garlic extract, suggesting that the antimicrobial activity of garlic extract is mainly due to DAS. An A. actinomycetemcomitans biofilm-killing assay performed with DAS showed a significant reduction in biofilm cell numbers, as evidenced by both confocal microscopy and culture. Scanning electron microscopy (SEM) analysis of DAS-treated A. actinomycetemcomitans biofilms showed alterations of colony architecture indicating severe stress. Flow cytometry analysis of OBA9 cells did not demonstrate apoptosis or cell cycle arrest at therapeutic concentrations of DAS (0.01 and 0.1 g/ml). DAStreated A. actinomycetemcomitans cells demonstrated complete inhibition of glutathione (GSH) S-transferase (GST) activity. However, OBA9 cells, when exposed to DAS at similar concentrations, showed no significant differences in GST activity, suggesting that DAS-induced GST inhibition might be involved in A. actinomycetemcomitans cell death. These findings demonstrate that DAS exhibits significant antibacterial activity against A. actinomycetemcomitans and that this property might be utilized for exploring its therapeutic potential in treatment of A. actinomycetemcomitans-associated oral and nonoral infections.

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Section: Discussionmentioning

confidence: 99%

In Vitro Efficacy of Diallyl Sulfides against the Periodontopathogen Aggregatibacter actinomycetemcomitans

Velliyagounder

Ganeshnarayan

Velusamy

et al. 2012

Antimicrob Agents Chemother

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“…The study of Chung et al (1998) inhibit both susceptible and drug-resistant H. pylori. Previous studies reported that DADS, DAT and PA could effectively inhibit the growth of several pathogens responsible for nosocomial infection including MRSA, Klebsiella pneumoniae and Pseudomonas aeruginosa (Tsao and Yin, 2001;Tsao et al, 2003;Liu et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…The in vitro and in vivo inhibitory activities of these sulphides against nosocomial pathogens such as methicillin-resistant Staphylococcus aureus (MRSA), Klebsiella pneumoniae, Pseudomonas aeruginosa have been studied in our laboratory (Tsao and Yin, 2001;Tsao et al, 2003). It has been reported that DADS and/or DAT could inhibit the growth of susceptible H. pylori (Chung et al, 1998;O'Gara et al, 2000). However, it remains unknown whether these agents could inhibit drug resistant H. pylori.…”

Section: Introductionmentioning

confidence: 99%

In vitro anti‐Helicobacter pylori activity of diallyl sulphides and protocatechuic acid

Liu

Hsu

Yin

2007

Phytotherapy Research

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The in vitro inhibitory effects of diallyl disulphide (DADS), diallyl trisulphide (DAT), roselle calyx extract and protocatechuic acid (PA) on the growth of Helicobacter pylori (15 susceptible, 11 clarithromycin-resistant and 9 metronidazole-resistant strains) were studied. The inhibition zone was determined after each agent had been heated at 25, 60, 100°C for 60 min. The minimal inhibitory concentration (MIC) of each agent was determined by the tube dilution assay. The results showed that heat treatment did not affect the anti-H. pylori activity of DADS, DAT, roselle calyx extract and PA, and the MIC values of these agents against test H. pylori strains were in the range 8 -64 mg/L. The time-kill study assay for DAT and PA at 1× × × × × MIC was monitored in Muller Hinton broth supplemented with 10% horse blood or mice stomach homogenate. Both DAT and PA inhibited the growth of all test H. pylori in broth and mice stomach homogenate ( p < < < < < 0.05); however, the inhibitory effects of these two agents were less in mice stomach homogenate than in broth (p < < < < < 0.05). DAT at 4, 6, 8, 10, 12, 14 mg/L and PA at 8, 16, 24, 32, 40, 48 mg/L were used for urease activity assay. These two agents significantly reduced urease activity of test H. pylori strains (p < < < < < 0.05), in which DAT and PA at 1× × × × × MIC reduced the urease activity of H. pylori to 70% and 40%, respectively. These agents, based on their lower MIC values and heat tolerance, might be useful in the prevention or therapy of H. pylori.

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“…Diallyl sulfide (DAS), one of the component of garlic, has been reported to act as a potential chemopreventive function for many human cancer such as colon, lung, and skin (24). In our earlier studies, DAS inhibited the N-acetyltransferase activity in bacteria (25,26), human colon cancer cells (27) and bladder cancer cells (28), and inhibits murine WEHI-3 leukemia cells in BALB/c mice in vitro and in vivo (29). However, the mechanistic role of DAS-mediated apoptosis in human cervical cancer cells, especially the role of ER stress, caspases, and mitochondria in the induction of apoptosis remains limited.…”

Section: Introductionmentioning

confidence: 95%

Diallyl sulfide induces cell cycle arrest and apoptosis in HeLa human cervical cancer cells through the p53, caspase- and mitochondria-dependent pathways

Chung

Liu

et al. 2011

Int J Oncol

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Abstract. Diallyl sulfide (DAS), one of the main active constituents of garlic, causes growth inhibition of cancer cells in vitro and promotes immune responses in vivo in experimental settings. However, its effects on the induction of cell cycle and apoptosis in human cervical cancer cells are still unclear. The aims of this study were to explore the anti-cancer effects of DAS in HeLa human cervical cancer cells and to investigate the underlying mechanisms in vitro. Cytotoxicity and apoptosis in HeLa human cervical cancer cells were examined by the morphological changes, viability assay, 4',6-Diamidino-2-phenylindole dihydrochloride (DAPI) staining, comet assay, Western blotting and confocal microscopy examination. The results showed that DAS treatment for 24-72 h resulted in a marked decrease in cell viability time-and dose-dependently. Flow cytometric analysis showed that a 48-h treatment of 75 µM DAS induced G0/G1 cell cycle arrest and sub-G1 phase (apoptosis) in HeLa cells. Typical apoptotic nucleus alterations were observed by fluorescence microscopy in HeLa cells after exposure to DAS using DAPI staining. Cells treated with different concentrations of DAS also showed changes typical of apoptosis such as morphological changes, DNA damage and fragmentation, dysfunction of mitochondria, cytochrome c release and increased expression of pro-caspase-3 and -9. DAS also promoted the release of AIF and Endo G from mitochondria in HeLa cells. In conclusion, DAS induced G0/G1 cell cycle arrest and apoptosis in HeLa cells through caspase-and mitochondria and p53 pathways providing further understanding of the molecular mechanisms of DAS action in cervical cancer. This study, therefore, revealed that DAS significantly inhibits the growth and induces apoptosis of human cervical cancer HeLa cells in vitro.

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Effects of Garlic Compounds Diallyl Sulfide and Diallyl Disulfide on Arylamine N-Acetyltransferase Activity in Strains ofHelicobacter pylorifrom Peptic Ulcer Patients

Cited by 57 publications

References 34 publications

In Vitro Efficacy of Diallyl Sulfides against the Periodontopathogen Aggregatibacter actinomycetemcomitans

In Vitro Efficacy of Diallyl Sulfides against the Periodontopathogen Aggregatibacter actinomycetemcomitans

In vitro anti‐Helicobacter pylori activity of diallyl sulphides and protocatechuic acid

Diallyl sulfide induces cell cycle arrest and apoptosis in HeLa human cervical cancer cells through the p53, caspase- and mitochondria-dependent pathways

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