Effects of gamma radiation on biodegradation of Bombyx mori silk fibroin

Kojthung, Amornthep; Meesilpa, Prateep; Sudatis, Boonya; Treeratanapiboon, Lertyot; Udomsangpetch, Rachanee; Oonkhanond, Bovornlak

doi:10.1016/j.ibiod.2007.12.012

Cited by 57 publications

(42 citation statements)

References 9 publications

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“…Aqueous silk solutions can be sterilized by a number of methods including sterile filtration, gamma irradiation, and autoclaving. Unfortunately, the more rigorous procedures, such as gamma irradiation 56,57 and autoclaving (unpublished results), have been shown to degrade the molecular weight of the silk fibroin and therefore irradiation dosing or steam sterilization timing should be worked out for each system. In some cases, it is simpler to sterilize the final product before use.…”

Section: Introductionmentioning

confidence: 99%

Materials fabrication from Bombyx mori silk fibroin

et al. 2011

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Silk fibroin, derived from Bombyx mori cocoons, is a widely used and studied protein polymer for biomaterial applications. Silk fibroin has remarkable mechanical properties when formed into different materials, demonstrates biocompatibility, has controllable degradation rates from hours to years, and it can be chemically modified to alter surface properties or to immobilize growth factors. A variety of aqueous or organic solvent processing methods can be used to generate silk biomaterials for a range of applications. In this protocol we include methods to extract silk from B. mori cocoons in order to fabricate hydrogels, tubes, sponges, composites, fibers, microspheres and thin films. These materials can be used directly as biomaterials for implants, as scaffolding in tissue engineering and in vitro disease models, and for drug delivery.

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Section: Introductionmentioning

confidence: 99%

Materials fabrication from Bombyx mori silk fibroin

et al. 2011

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“…Kojthung et al . (2008) gamma‐irradiated silk fibroin fibers (up to 3000 kGy) and indicated that γ‐rays did not act on primary structure of protein, but induced a faster biodegradation . Furthermore, Byun et al .…”

Section: Discussionmentioning

confidence: 99%

“…[75][76][77][78][79] Lawrence et al (2008) observed that after steam sterilization, silk fibroin films presented FTIR peaks shifted to lower wavenumbers, indicating a b-sheet increase. 67 Kojthung et al (2008) gamma-irradiated silk fibroin fibers (up to 3000 kGy) and indicated that c-rays did not act on primary structure of protein, but induced a faster biodegradation. 80 Furthermore, Byun et al (2010) observed an antitumor potentiality of gamma-irradiated silk fibroin solution (up to 200 kGy).…”

Section: Discussionmentioning

confidence: 99%

“…67 Kojthung et al (2008) gamma-irradiated silk fibroin fibers (up to 3000 kGy) and indicated that c-rays did not act on primary structure of protein, but induced a faster biodegradation. 80 Furthermore, Byun et al (2010) observed an antitumor potentiality of gamma-irradiated silk fibroin solution (up to 200 kGy). 81 The results from FTIR analysis before sterilization performed on sample SF1, indicate that sterilization does not induce fibroin conformation change.…”

Section: Discussionmentioning

confidence: 99%

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Platelet lysate and adipose mesenchymal stromal cells on silk fibroin nonwoven mats for wound healing

Chlapanidas

Perteghella

Faragò

et al. 2015

J of Applied Polymer Sci

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In this work platelet lysate (PL) and adipose‐derived mesenchymal stromal cells (ASCs) seeded on nonwoven fibroin mats were in vitro and in vivo evaluated for tissue regenerative applications. Nonwoven mats obtained by a large scale water entanglement technique were characterized for their physico‐chemical properties. Results indicated a high purity of fibroin fibers, their stability after sterilization process and appropriate technological properties suitable for tissue engineering. Moreover, the scaffolds in vitro supported adhesion and migration of ASCs and the presence of PL improved the cell proliferation. The products were then applied on epithelial/dermal wounds carried out on the dorsal surface of rabbit: the skin reparative process was solved in 9 days, with a completely restitutio ad integrum of the epithelium in animals treated with PL alone; ASCs did not further improve the wound healing. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 42942.

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“…Moreover, our results indicate that after gamma radiation a less energy was requested for fibroin degradation, probably due to a peptide bond weakening. Kojthung et al (42) evaluated that gamma radiation reduces silk fibroin tensile strength, molecular weight, and B-sheet content: this effect is more pronounced by increasing radiation intensity. On the other side, George et al (43) observed that gamma radiation is the most suitable method for the sterilization of silk fibroin films for corneal tissue engineering.…”

Section: Discussionmentioning

confidence: 99%

Formulation and Characterization of Silk Fibroin Films as a Scaffold for Adipose-Derived Stem Cells in Skin Tissue Engineering

Chlapanidas

Tosca

Faragò

et al. 2013

Int J Immunopathol Pharmacol

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Skin substitutes are epidermal, dermal or complete bilayered constructs, composed by natural or synthetic scaffolds and by adherent cells such as fibroblasts, keratinocytes or mesenchymal stem cells. Silk fibroin is a promising polymer to realize scaffolds, since it is biocompatible, biodegradable, and exhibits excellent mechanical properties in terms of tensile strength. Moreover, fibroin can be added of others components in order to modify the biomaterial properties for the purpose. The aim of this work is to prepare silk fibroin films for adipose-derived stem cell (ADSCs) culture as a novel feeder layer for skin tissue engineering. Pectin has been added to promote the protein conformational transition and construct strength, while glycerol as plasticizer, providing biomaterial flexibility. Eighteen formulations were prepared by casting method using fibroin, pectin (range 1-10% w/w), and glycerol (range 0-20% w/w); films were characterized by Fourier transform infrared spectroscopy and differential scanning calorimetry assay, to select the optimal composition. A stable fibroin conformation was obtained using 6% w/w pectin, and the best mechanical properties were obtained using 12% w/w glycerol. Films were sterilized, and human ADSCs were seeded and cultured for 15 days. Cells adhere to the support assuming a fibroblastic-like shape and reaching confluence. The ultrastructural analysis evidences typical active-cell features and adhesion structures that promote cell anchorage to the film, thus developing a multilayered cell structure. This construct could be advantageously employed in cutaneous wound healing or where the use of ADSCs scaffold is indicated either in human or veterinary field.The development of skin substitutes dates back to 1975, when Rheinwald and Green (1) cultured keratinocytes on a layer of lethally irradiated 3T3 murine fibroblasts. Currently, this method remains the most reliable and widely used for in vitro keratinocyte culture, but some concerns are emerging about their safety (2-5). To overcome these limits, Sugiyama et al. (6) proposed the use of human irradiated adipose-derived stem cells (ADSCs) as a feeder cell layer: results showed that the morphology of irradiated stem cells was similar to 3T3, and both cell lines (stem and 3T3 cells) expressed genes promoting keratinocyte proliferation. Other researchers also showed that non-irradiated ADSCs can boost epithelialization, angiogenesis (7), capillary density and granulation thickness of transplanted cell-enriched collagen sponges (8). Altman et al. (9) observed that ADSCs, once seeded on acellular dermal matrix and applied on murine wound, spontaneously differentiated along vascular endothelial, fibroblastic and epidermal epithelial lineages and significantly improved wound healing; moreover, cells localize at implantation site.Current cutaneous substitutes are epidermal, dermal or

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Effects of gamma radiation on biodegradation of Bombyx mori silk fibroin

Cited by 57 publications

References 9 publications

Materials fabrication from Bombyx mori silk fibroin

Materials fabrication from Bombyx mori silk fibroin

Platelet lysate and adipose mesenchymal stromal cells on silk fibroin nonwoven mats for wound healing

Formulation and Characterization of Silk Fibroin Films as a Scaffold for Adipose-Derived Stem Cells in Skin Tissue Engineering

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