Effects of Gamma-Linolenic Acid Supplementation on Lipid Composition of Liver Microsomal Membranes

DIB, Amale; CLAVEL, Jean-Pierre; CARREAU, Jean-Paul

doi:10.3164/jcbn.6.95

Cited by 5 publications

(6 citation statements)

References 2 publications

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“…Table 3. Effects of zinc fortification for 20 weeks on plasma total phospholipid fatty acid composition of 6-to 10-year-old Beninese children † (Mean values and standard deviations; medians and interquartile ranges (IQR); β-coefficients and 95 % confidence intervals) lower n-6 LCPUFA in the blood/tissue of Zn-deficient animals, indicating a disruption in desaturation and/or elongation of FA (8,15,18,19,49) . A novel finding of this study is that Zn fortification prevented the reduction of nervonic acid, a MUFA.…”

Section: Discussionmentioning

confidence: 99%

“…Zn deficiency altered the FA composition of phospholipids in rat liver, plasma and erythrocytes (8,(10)(11)(12)(13)(14) . Some studies have reported higher levels of LA (18 : 2n-6) (12,(15)(16)(17) whilst some have reported lower levels of arachidonic acid (AA, 20 : 4n-6) (11,15,16,(18)(19)(20) and others high or normal levels of AA in tissue phospholipid of Zn-deficient animals. This relationship was also shown in a recent human study (21) .…”

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confidence: 99%

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Sensitivity of fatty acid desaturation and elongation to plasma zinc concentration: a randomised controlled trial in Beninese children

et al. 2018

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Zn status may affect fatty acid (FA) metabolism because it acts as a cofactor in FA desaturase and elongase enzymes. Zn supplementation affects the FA desaturases of Zn-deficient rats, but whether this occurs in humans is unclear. We evaluated the associations between baseline plasma Zn (PZn) concentration and plasma total phospholipid FA composition, as well as the effect of daily consumption of Zn-fortified water on FA status in Beninese children. A 20-week, double-blind randomised controlled trial was conducted in 186 school age children. The children were randomly assigned to receive a daily portion of Zn-fortified, filtered water delivering on average 2·8 mg Zn/d or non-fortified filtered water. Plasma total phospholipid FA composition was determined using capillary GLC and PZn concentrations by atomic absorption spectrometry. At baseline, PZn correlated positively with dihomo-γ-linolenic acid (DGLA, r 0·182; P=0·024) and the DGLA:linoleic acid (LA) ratio (r 0·293; P<0·000), and negatively with LA (r -0·211; P=0·009) and the arachidonic acid:DGLA ratio (r -0·170; P=0·036). With the intervention, Zn fortification increased nervonic acid (B: 0·109; 95 % CI 0·001, 0·218) in all children (n 186) and more so in children who were Zn-deficient (n 60) at baseline (B: 0·230; 95 % CI 0·023, 0·488). In conclusion, in this study, Zn-fortified filtered water prevented the reduction of nervonic acid composition in the plasma total phospholipids of children, and this effect was stronger in Zn-deficient children. Thus, Zn status may play an important role in FA desaturation and/or elongation.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Sensitivity of fatty acid desaturation and elongation to plasma zinc concentration: a randomised controlled trial in Beninese children

et al. 2018

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“…Fatty acid delta-9 or delta-6 desaturating activities were measured in the microsomal fraction of carcinoma, as described elsewhere (Dib et al, 1989). Tumor tissue specimens were homogenized at 4°C and the microsomal fraction was prepared from the 100,000 g pellet.…”

Section: Enzyme Assaysmentioning

confidence: 99%

“…Radioactive spots were scraped off into a scintillation mixture and counted. Liver microsomal fractions prepared from starved or re-fed rats were used under the same conditions as negative and positive controls, respectively (Dib et al, 1989).…”

Section: Enzyme Assaysmentioning

confidence: 99%

Membrane fatty acids of breast carcinoma: Contribution of host fatty acids and tumor properties

Chajès

Lanson

Fetissof³

et al. 1995

Intl Journal of Cancer

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To assess the part that host fatty-acid supply and tumor-specific fatty-acid utilization contribute to the membrane lipid composition of tumor tissue, intra-individual comparisons of membrane fatty acids were carried out between breast-carcinoma tissue and non-tumorous breast tissue adjacent to the tumor and taken as reference. Phospholipids were purified by thin-layer chromatography from tumor biopsies obtained from 59 patients with a localized presentation of breast cancer, and fatty acids analyzed by capillary gas chromatography. Elevated levels of palmitic, palmitoleic and arachidonic acids, along with a low level of linoleic acid, were observed in membrane phospholipids of tumors with poor histoprognostic grade. The level of mono-unsaturated fatty acids was higher, and the level of essential fatty acids was lower in the tumor than in the reference breast tissue. Fatty-acid-desaturating activity was not detectable in tumors. A positive relationship was observed among patients for most of the fatty acids between carcinoma and non-tumorous breast tissue, except for mono-unsaturated and essential fatty-acid levels, which were not correlated between both tissues. These data suggest that mechanisms specifically related to malignant transformation and tumor progression influence the membrane fatty-acid profile of breast carcinoma. Fatty acid supply to the tumor, possibly modified by metabolic conditions related to the host, also seems to play a decisive role in this composition.

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“…The administration of GLA during chronic ethanol intake in rats has beneficial effects, normalizing the lipid composition of erythrocyte and hepatocyte membranes that can be explained by overproduction of hepatoprotective eicosanoids (Quoc and Pascaud, 1996). It has been shown that GLA normalizes the phospholipids composition of microsomal membranes in pregnant rats and fetuses fed on a Zn-deficient diet (Dib et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

Reversal of Experimental Ethanol‐induced Liver Steatosis by Borage Oil

Lukivskaya

Naruta

Sadovnichy

et al. 2012

Phytotherapy Research

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The aim of study was to evaluate the hepatoprotective effect of borage oil containing predominantly gamma-linolenic acid in rats with alcoholic steatohepatitis. Liver of ethanol-treated animals was characterized by fatty and hydropic dystrophies. Liver triglyceride contents and activitiies of serum marker enzymes were significantly increased. Ethanol increased nicotinamide adenine dinucleotide phosphate hydrogen (NADPH)-induced chemiluminescence and the contents of liver thiobarbituric acid reactive substances (TBARS). The reduced glutathione content in the liver was decreased. Ethanol enhanced liver microsomal cytochrome P-450 (CYP450) content, aniline p-hydroxylase and amydopyrine-N-demethylase activities. The treatment with borage oil improved the liver morphology, decreased triglyceride contents and normalized serum marker enzyme activities. Borage oil developed an antioxidant effect in ethanol-treated rats. The treatment with this compound decreased NADPH-induced chemiluminescence and the content of lipid peroxidation products. Borage oil normalized CYP450 content compared with the ethanol-treated group. CYPI450 2E1 isoform is a main source of free oxygen radicals in the liver of ethanol-treated rats and we propose that the antioxidant effect of borage oil is realized via the normalization of CYP450 content and activities of CYP450-related microsomal oxidases, as borage oil can improve the lipid surrounding of CYP450. In our opinion, the hepatoprotection by borage oil in alcoholic steatosis is connected with its antioxidant properties.

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Effects of Gamma-Linolenic Acid Supplementation on Lipid Composition of Liver Microsomal Membranes

Cited by 5 publications

References 2 publications

Sensitivity of fatty acid desaturation and elongation to plasma zinc concentration: a randomised controlled trial in Beninese children

Sensitivity of fatty acid desaturation and elongation to plasma zinc concentration: a randomised controlled trial in Beninese children

Membrane fatty acids of breast carcinoma: Contribution of host fatty acids and tumor properties

Reversal of Experimental Ethanol‐induced Liver Steatosis by Borage Oil

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