Effects of gamma irradiation and periodate oxidation on the structure of dextrin assessed by mass spectrometry

Abstract

A B S T R A C TSterilization of biomaterials by gamma irradiation should not change its structure, since this may affect also its bioactivity. In this work, dextrin and periodate-oxidized dextrin (ODEX), both irradiated and non-irradiated, were hydrolysed by α-amylase and the obtained oligosaccharides were fractionated by ligand-exchange/size exclusion chromatography and analyzed by matrix-assisted laser desorption/ionization (MALDI-MS) and electrospray ionization (ESI-MS n ) mass spectrometry. This allowed the identification of structures formed during partial periodate oxidation, which displayed aldehyde groups in different positions of dextrin backbone, according to the type of residue which was oxidized. Importantly, gamma irradiation did not structurally change either dextrin or ODEX, showing that it can be used as suitable terminal sterilization method of these materials for biomedical applications. To our best knowledge, this is the first report using MS-based techniques to evaluate the effects of partial periodate oxidation and of gamma irradiation on polysaccharides and, in particular, on dextrin and its oxidized form.to the formation of two aldehyde groups [11]. Initially, this method was routinely used in the characterization and elucidation of the polysaccharide structure through complete oxidation [11]. In later years, low and mild periodate oxidations have been used to functionalize (J. Simões), dmitrye@ua.pt (D.V. Evtyugin), sophie.rouif@ionisos.fr (S. Rouif), mac@ua.pt (M.A. Coimbra), mrd@ua.pt (M.R.M. Domingues), fmgama@deb.uminho.pt (M. Gama).

In vivo systemic toxicity assessment of an oxidized dextrin‐based hydrogel and its effectiveness as a carrier and stabilizer of granular synthetic bone substitutes

“…This is the case of dextran 67,68 and hydroxyethyl starch, 69,70 both used as plasma volume expanders. 25 The obtained results suggest that such modification did not promote any skin sensitization. 36,37 It is generally safe and well tolerated by PD patients, but there are few reports of acute self-limiting allergic hypersensitivity reaction to icodextrin, such as skin rashes.…”

“…Dextrin oxidation was performed as previously described by Pereira et al 25 Briefly, aqueous solutions of dextrin (2% w/v) were oxidized with sodium m-periodate, to yield the theoretical degree of oxidation of 40%, at room temperature, with stirring, in the dark. Dextrin oxidation was performed as previously described by Pereira et al 25 Briefly, aqueous solutions of dextrin (2% w/v) were oxidized with sodium m-periodate, to yield the theoretical degree of oxidation of 40%, at room temperature, with stirring, in the dark.…”

“…Dextrin oxidation. Dextrin oxidation was performed as previously described by Pereira et al 25 Briefly, aqueous solutions of dextrin (2% w/v) were oxidized with sodium m-periodate, to yield the theoretical degree of oxidation of 40%, at room temperature, with stirring, in the dark. After 20 h, the oxidation reaction was stopped by adding drop wise an equimolar amount of diethylene glycol, to reduce any unreacted periodate.…”

In vitro genotoxicity assessment of an oxidized dextrin‐based hydrogel for biomedical applications

“…Dextrin oxidation was performed as described by Pereira et al (). Briefly, aqueous solutions of dextrin (2% w/v) were oxidized with sodium m ‐periodate, to yield the theoretical degree of oxidation of 40%, at room temperature, with stirring, and in the dark.…”

“Novel Dextrin‐Cysteine Schiff Base: A Highly Efficient Sensor for Mercury Ions in Aqueous Environment”

“…The formation of DCS is confirmed by HRMS spectral analysis of dextrin (DEX) and DCS where the corresponding figures are shown in S1 and S2 respectively. The Figure S1 (a, b) shows that the peaks at m/z =169.04 and 203.00 correspond to the [C 6 H 10 O 4 +Na] + and [C 6 H 12 O 6 +Na] + moieties respectively, indicating the presence of anhydrous glucose unit (AGU) in dextrin polysaccharide . However the Figure S2 (a, b) shows that the new peaks appear at m/z = 133.02 and 107.04 that correspond to [C 4 H 6 NO 2 S+H] + and [C 3 H 6 O 2 S+H] + units.…”