Effects of Gaba on the Thymus Cytokines of Wenchang Chickens Submitted to Heat Stress

Xie, XZ; Chen, Liang; Mh, Li; Chen, Z

doi:10.1590/1806-9061-2016-0334

Cited by 5 publications

(3 citation statements)

References 17 publications

(13 reference statements)

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“…Furthermore, IFN-γ plasma levels were significantly lower in the TM group at 0 h, compared to the controls, and AHS increased plasma IFN-γ levels only in TM chicks. Correspondingly, previous studies have reported increased IFN-γ expression in chickens during heat stress conditions [14,41]. In contrast, another study has showed that heat-stressed broiler chickens possessed lower levels of IFN-γ expression [5].…”

Section: Discussionmentioning

confidence: 88%

Effect of Acute Heat Stress on the mRNA Levels of Cytokines in Broiler Chickens Subjected to Embryonic Thermal Manipulation

Saleh

Al‐Zghoul

2019

Animals

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Heat stress significantly impacts the immunity and cytokine expression of chickens. However, the effects of embryonic thermal manipulation (TM) on cytokine expression in broiler chickens (broilers) is unclear. The objective of the current study was to evaluate the effects of TM on the splenic mRNA expression dynamics of certain cytokines—namely, IFN-α, IFN-β, IFN-γ, IL-4, IL-8, IL-15, IL-16, IL-17, and IL-18—in broilers during subsequent exposure to acute heat stress (AHS). TM was performed by elevating the incubation temperature to 39 °C at 65% relative humidity (RH) for 18 h daily during embryonic days (ED) 10–18. On post-hatch day 28, AHS was carried out for 7 h at 40 °C. At 0 h and after 1, 3, 5, and 7 h of AHS, splenic tissues were collected from all study groups to evaluate mRNA expression by relative-quantitative real-time (RT)-PCR. Plasma was collected to measure IL-4, IL-8, and IFN-γ levels. At 0 h, TM significantly reduced the basal mRNA level of IFN-β and the plasma level of IFN-γ and IL-8. Moreover, AHS significantly decreased IFN-β in control chicks, decreased IL-4 in both TM and control chicks, and increased IFN-γ and IL-16 in TM chicks. IFN-α, IL-8, IL-15, IL-17, and IL-18 expression all significantly increased during AHS in both TM and control chicks, but expression dynamics were improved in TM chicks for all cytokines (except IL-17). AHS resulted in increased plasma IFN-γ levels in TM chicks only, and increased IL-8 levels at 3 and 5 h of AHS in TM chicks, but at 7 h in control chicks. Lastly, 3 h of AHS increased IL-4 plasma levels in control chicks. The results of this study may indicate that TM has a long-term effect on cytokine expression dynamics of broilers, especially during AHS. Therefore, TM may improve heat tolerance acquisition by increasing the expression of signaling proteins important to tissue stability and to repair mechanisms that are employed during and/or after heat stress recovery.

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Section: Discussionmentioning

confidence: 88%

Effect of Acute Heat Stress on the mRNA Levels of Cytokines in Broiler Chickens Subjected to Embryonic Thermal Manipulation

Saleh

Al‐Zghoul

2019

Animals

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“…Recent studies have found that oxidative stress can cause damage to the antioxidant enzyme system of human chorionic trophoblast cells [ 26 ]. Research on GABA in livestock and poultry production mainly focuses on resisting heat stress and regulating appetite [ 27 , 28 , 29 ], but the effects of GABA on alleviating the oxidative stress of embryonic trophoblast cells are still unknown. Our results found that oxidative stress induced by H 2 O 2 reduced the activity of SOD, CAT, and GSH-Px, while the 100 and 200 μmol/L GABA pretreatments can significantly increase the activity of these enzymes.…”

Section: Discussionmentioning

confidence: 99%

Maternal Dietary Supplementation with γ-Aminobutyric Acid Alleviated Oxidative Stress in Gestating Sows and Their Offspring by Regulating GABRP

Liu

Jiang

Pang

et al. 2022

Animals

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Sows usually suffer oxidative stress during gestation, and this limits the growth of fetuses via placenta. Gamma-aminobutyric acid (GABA) is a functional nonessential amino acid engaged in regulating the physiological status of animals. However, the effects of GABA on the oxidative homeostasis of sows and their offspring remain unclear. Eighteen late gestating sows (85 d) were divided into the CON and GABA groups and fed the basal diet and the GABA diet (200 mg/kg GABA), respectively, until farrowing. At parturition, the sows’ litter characteristics, the plasma antioxidant parameters of sows, and their offspring were evaluated. The results showed that GABA supplementation had no marked effect on the reproductive performance of sows (p > 0.10) but had a trend of reducing the amount of intrauterine growth restriction (IUGR) in piglets (0.05 < p < 0.10). At the same time, the addition of GABA elevated the plasma superoxide dismutase (SOD) level of sows and enhanced the glutathione peroxidase (GSH-Px) activity of newborn piglets (p < 0.05). Based on the H2O2-induced oxidative stress in pTr-2 cells, GABA elevated intracellular GSH-Px, SOD, catalase (CAT), and total antioxidant capacity (T-AOC, p < 0.01) and upregulated the gene expressions of CAT, gamma-aminobutyric acid receptor (GABRP), and nuclear factor-erythroid 2-related factor-2 (Nrf2) in H2O2-treated pTr-2 cells (p < 0.05). Taken together, GABA improved the antioxidant capacity of sows and alleviated the placental oxidative stress by upregulating the GABRP and Nrf2 genes, which have the potential to promote oxidative homeostasis in newborn piglets.

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“…In laying hens, supplementation with GABA has been found to positively affect egg quality in terms of improved laying hen quality [6]. It was also found that GABA increases the immune responses in chickens during stress conditions [7]. Therefore, GABA is a valuable metabolite that can be applied to humans and livestock.…”

Section: Introductionmentioning

confidence: 99%

Gamma-Aminobutyric Acid (GABA) Producing Lactobacillus plantarum TSUB-17 and Probiotic Properties for Using as Probiotics Additive in Swine Feed

Khongkool,

Prakit,

Chaiyod

et al. 2023

Trends Sci

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Due to the use of probiotics in animal husbandry has recently increased, the selection of high-quality probiotic strains is crucial. Since the uses of probiotics for animal feed supplements have to be applied at high doses of bacteria to be effective, the cultures’ safety is essential. This study successfully isolated the novel Lactobacillus sp. from swine fecal samples and identified it as L. plantarum TSUB-17. The L. plantarum TSUB-17 exhibited excellent probiotic properties, including 100 % resistance to 3 % acids and 1 % bile salts, and effectively inhibits pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae and Salmonella Typhimurium (isolated ESBL strain). L. plantarum TSUB-17 was also found to adhere to the intestinal wall and form a biofilm. Furthermore, the bacteria have revealed some unique properties of GABA production and detected the glutamate decarboxylase B (gadB) gene similar to L. brevis both in nucleotide sequence and secondary structure of the glutamate decarboxylase enzyme that was deduced from the nucleotide. The isolated bacteria also have antioxidant activity, and most importantly, the isolated strain was safe; it is non-hemolysis and susceptible to the tested antimicrobials. Therefore, L. plantarum TSUB-17 is suitable for further development as a probiotic additive in animal feed. HIGHLIGHTS This study successfully isolated Lactobacillus plantarum TSUB-17 with a high potential to develop as a probiotic for animals. The isolated bacterium has excellent properties: It tolerates high levels of acids and bile salts. It is effective against many pathogenic bacteria, produces gamma-aminobutyric acid (GABA) and shows high levels of antioxidant activity. Moreover, L. plantarum TSUB-17 is very safe due to its non-hemolytic strain and susceptibility to numerous antimicrobial drugs. GRAPHICAL ABSTRACT

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Effects of Gaba on the Thymus Cytokines of Wenchang Chickens Submitted to Heat Stress

Cited by 5 publications

References 17 publications

Effect of Acute Heat Stress on the mRNA Levels of Cytokines in Broiler Chickens Subjected to Embryonic Thermal Manipulation

Effect of Acute Heat Stress on the mRNA Levels of Cytokines in Broiler Chickens Subjected to Embryonic Thermal Manipulation

Maternal Dietary Supplementation with γ-Aminobutyric Acid Alleviated Oxidative Stress in Gestating Sows and Their Offspring by Regulating GABRP

Gamma-Aminobutyric Acid (GABA) Producing Lactobacillus plantarum TSUB-17 and Probiotic Properties for Using as Probiotics Additive in Swine Feed

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