Effects of GA₃Pregerminative Treatment onGentiana luteaL. var.aurantiacaGermination and Seedlings Morphology

Abstract

Gentiana lutea L. is widely used in bitter beverages and in medicine; Gentianae Radix is the pharmaceutical name of the root of G. lutea. These uses have generated a high demand. The wild populations of Gentiana lutea var. aurantiaca (M. Laínz) M. Laínz have been decimated; it is necessary to establish guidelines for its cultivation. Gentian as most alpine species has dormant seeds. Dormancy can be removed by cold and by means of a gibberellic acid (GA3) treatment. However, cold treatments produce low germination percentages and GA3 treatments may produce off-type seedlings. So, the objective was to determine, for the first time, the presowing treatments that allow high germination rate and good seedling morphology. The best pregerminative doses of GA3 to break seed dormancy were 100, 500, and 1000 ppm, while the best doses to optimize the seedling habit were 50 and 100 ppm. This study provides, for the first time, a 100 ppm GA3 dose that led to a high germination rate and good seedling morphology, as the starting point for gentian regular cultivation.

Non-Deep Simple Morphophysiological Dormancy and Germination Characteristics of Gentiana triflora var. japonica (Kusn.) H. Hara (Gentianaceae), a Rare Perennial Herb in Korea

“…In a previous study, dormancy breaking and germination were improved by applying GA 3 to Gentiana species; this has also been observed in numerous other plant species [22,[43][44][45][46]. Similarly, in this study, the final germination was enhanced by the application of 100 and 1000 mg•L −1 exogenous GA 3 at 90.0 ± 2.0% and 84.0 ± 4.3%, respectively (Figure 6A).…”

“…In a previous study, GA 3 application was more effective at 1000 mg•L −1 than at 50 and 100 mg•L −1 and positively affected the percentage and speed of germination in G. lutea L. var. aurantiaca seeds [43]. In addition, 250 mg•L −1 GA 3 remarkably influenced the germination percentage compared to the results of 3-month cold stratification in G. lutea L. subsp.…”

Slow Release of GA3 Hormone from Polymer Coating Overcomes Seed Dormancy and Improves Germination

“…The addition of GA to the seed may also promote the activation of enzymes, cell expansion, and the mobilization of stored reserves, which collectively facilitate the transition from dormancy to germination [ 15 ]. Gibberellic acid (GA 3 ) is a common gibberellin applied as a seed treatment [ 18 , 19 , 20 ]. It is typically used by imbibing the seeds in a solution containing the hormone through priming [ 21 , 22 ].…”

MORPHO - PHYSIOLOGICAL CHARACTERISTICS OF GENTIAN (Gentiana lutea L.) GENOTYPE SEEDLINGS FROM NATURAL POPULATIONS IN MONTENEGRO

“…Sustainable use of natural resources can only be achieved with the introduction of plants, as well as cultivation, which is an important way to protect endangered plant species (Menges, 2000). Therefore, in recent decades of the last and at the beginning of this century, abroad, but also in Serbia and Slovenia great efforts had been made to develop a technology for gentian cultivation, especially in rural mountainous areas (Kušar and Baričević, 2006;Radanović et al, 2007b;2007c;Radanović, 2008;Franz, 2012;Radanović et al, 2013;Balijagić, 2013;González-López et al, 2014;Sand, 2015).…”