Effects of furfural and acetic acid on growth and lipid production from glucose and xylose by Rhodotorula glutinis

Zhang, Guochang; French, W. Todd; Hernández, Rafael; Alley, Earl G.; Paraschivescu, Maria C.

doi:10.1016/j.biombioe.2010.10.009

Cited by 74 publications

(58 citation statements)

References 16 publications

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“…The other fatty acids were stearic acid (C18:0) (8.3 %), alpha-linolenic acid (C18:3) (5.9 %) and myristic acid (C14:0) (2.6 %). The fatty acid composition of R.glutinis SO28 was similar to those of other R.glutinis strains reported by previous studies [39][40][41]. The Table 4 Effect of additional phosphorus source on lipid accumulation in R. glutinis Waste Biomass Valor most prominent fatty acid of R. glutinis S028 was oleic acid.…”

Section: Determination Of Fatty Acids Composition Of the Yeastsupporting

confidence: 73%

Evaluation of Waste Loquat Kernels as Substrate for Lipid Production by Rhodotorula glutinis SO28

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Taşkın

et al. 2016

Waste Biomass Valor

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This study was performed to produce lipids from the isolated oleaginous yeast Rhodotorula glutinis SO28 using loquat kernel extract (LKE) as substrate. LKE was prepared using acid hydrolysis and alkaline neutralization steps. Lipid production was performed in shaking flaks culture. Even if LKE was used as a sole source of nutritional substances, it could support cell growth and lipid synthesis in the yeast. Additional carbon, nitrogen and phosphorus sources were found to significantly alter the lipid accumulation potential of the yeast. Optimal concentrations of additional carbon (glucose) and nitrogen (ammonium sulphate) sources for lipid accumulation were determined as 15 and 0.5 g/L, respectively. On the other hand, all the concentrations of additional phosphorus source were found to significantly reduce the lipid accumulation. Optimal incubation time was determined as 132 h. Under the optimized culture conditions, the lipid concentration and lipid content of the yeast were determined as 7.82 g/L and 62 %, respectively. Fatty acid methyl ester analysis exhibited that this yeast strain could produce high proportions of C16:0 and C18 fatty acids, which are ideal for biodiesel production. This is the first report on the use of waste loquat kernels as substrate for microbial lipid production.

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Section: Determination Of Fatty Acids Composition Of the Yeastsupporting

confidence: 73%

Evaluation of Waste Loquat Kernels as Substrate for Lipid Production by Rhodotorula glutinis SO28

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Yazıcı

Taşkın

et al. 2016

Waste Biomass Valor

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“…Determination of fats content in yeast biomass was carried out-after their prior centrifugation (2012×g/4 °C/10 min) and drying to constant weight-according to method of Bligh and Dyer modified by Zhang [51], involving lipids extraction using chloroform and methanol. Fats content was provided as % CDW.…”

Section: Lipid Analysismentioning

confidence: 99%

Evaluation of lipid biosynthesis ability by Rhodotorula and Sporobolomyces strains in medium with glycerol

Gientka

Gadaszewska

Błażejak

et al. 2016

Eur Food Res Technol

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“…The growth kinetics of the yeasts on different sugars as well as combination of sugars and inhibitors was studied as well. A simple model of exponential growth (with a lag phase) was used 21 , i.e.…”

Section: Resultsmentioning

confidence: 99%

“…Accumulation of carotenoid pigments typically occurs during stationary phase. However, the presence of inhibitors from hydrolysate in production medium may very much delay or even prevent the yeast cells from reaching this phase [21][22] . …”

mentioning

confidence: 99%

Monitoring of Growth and Production Characteristics of Red Yeasts Cultivated on Hydrothermally Pretreated Lignocellulosic Pine Material

Hároniková¹

2018

Chem.Biochem.Eng.Q.

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The aim of this work was to compare the production of carotenes and ergosterol by red yeasts grown on pine lignocellulose substrates. The yeast strains Rhodotorula aurantiaca and Sporobolomyces shibatanus were grown on the liquid fraction of steam pretreated pine (210 °C, catalyst SO 2 ). Biomass production on a pine hydrolysate was lower than on glucose. The highest content of carotenoids and ergosterol in the cells of R. aurantiaca grown on pine hydrolysate was about 1.7 mg g -1 and 0.8 mg g -1 (dwt), respectively, and in S. shibatanus about 0.9 mg g -1 and 0.1 mg g -1 , respectively. Hemicellulose hydrolysates may contain many compounds that have inhibitory effects on microorganisms. In this work, the influences of some inhibitors were assessed by cultivating yeasts on media with a representative addition of the selected compounds. From these tests, furfural appears to be the most critical inhibitor, whereas acetic acid and 5-hydroxymethyl furfural (HMF) do not affect the growth so much. Key words:lignocellulose, steam pretreatment, red yeasts, carotenoids, ergosterol IntroductionCarotenoids, such as unsaturated isoprene derivates, are the most distributed class of pigments in nature with orange, yellow or red colour. These pigments have a wide range of industrial applications. They provide attractive colour and antioxidant activity in food products, and some pigments act as vitamin A precursors in food and animal feed. Possible tumour inhibiting activity has been reported 1 , and also enhancement of the immune response leading to protection against infections 2 . Lycopene, a carotenoid pigment, could be useful in prevention of heart diseases and several types of cancers 3 . β-carotene has specific functions, such as photoprotection by light absorbing capacity, and acts as a precursor of vitamin A and specific hormones. It therefore reduces cell damage 4 . Carotenoids are produced by filamentous fungi and yeasts like Blakeslea trispora, Phaffia sp., Rhodotorula sp., and by some species of bacteria and algae, such as Dunaliella sp., and Flavobacterium sp. [5][6][7][8] Commercial interests are primarily directed towards the fungus Blakeslea sp. and yeasts Phaffia sp. and Rhodotorula sp.5 The microbial production of carotenoids is of interest mainly because of economic advantages of using low-cost natural substrates as sources of carbohydrates, compared with the direct extraction from vegetables or production by chemical synthesis 8,9 . Red yeasts are able to utilize a number of carbon sources, such as glucose, xylose, glycerol, and others [9][10][13][14] . To reduce the cost of biotechnological processes, the influence of process conditions have been studied, including effects of physical and nutritional factors, such as minerals, vitamins, aeration, pH, temperature, lighting or stress factors. Stress brings biochemical responses involving an increase in the activity of enzymes, which can result in increased production of carotenoids 10. A number of studies have investigated carotenoids production by use of che...

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Effects of furfural and acetic acid on growth and lipid production from glucose and xylose by Rhodotorula glutinis

Cited by 74 publications

References 16 publications

Evaluation of Waste Loquat Kernels as Substrate for Lipid Production by Rhodotorula glutinis SO28

Evaluation of Waste Loquat Kernels as Substrate for Lipid Production by Rhodotorula glutinis SO28

Evaluation of lipid biosynthesis ability by Rhodotorula and Sporobolomyces strains in medium with glycerol

Monitoring of Growth and Production Characteristics of Red Yeasts Cultivated on Hydrothermally Pretreated Lignocellulosic Pine Material

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