Effects of fumonisin B1 alone and combined with deoxynivalenol or zearalenone on porcine granulosa cell proliferation and steroid production

Cortinovis, C.; Caloni, F.; Schreiber, Nicole B.; Spicer, L. J.

doi:10.1016/j.theriogenology.2014.01.027

Cited by 70 publications

(50 citation statements)

References 49 publications

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“…Direct effects of DON on ovarian function have been documented in pigs (Ranzenigo et al, 2008;Medvedova et al, 2011;Cortinovis et al, 2014). However, knowledge about the effects of DON on ruminants is limited and literature on this issue is still rare (Seeling et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Presently, little information is available about the effects of Fusarium mycotoxins on ovarian function in cows. In pigs, direct ovarian effects of ␣-Zol has been well documented (Tiemann et al, 2003;Minervini et al, 2006;Ranzenigo et al, 2008;Cortinovis et al, 2014). Therefore, the purpose of this study was to determine, through the evaluation of cell proliferation and steroid production, if DON and ␣-Zol have effects on bovine large-follicle granulosa cells (LGGC).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Individual and combined effects of deoxynivalenol and α-zearalenol on cell proliferation and steroidogenesis of granulosa cells in cattle

Pizzo

Caloni

Schütz

et al. 2015

Environmental Toxicology and Pharmacology

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Individual and combined effects of deoxynivalenol and α-zearalenol on cell proliferation and steroidogenesis of granulosa cells in cattle

Pizzo

Caloni

Schütz

et al. 2015

Environmental Toxicology and Pharmacology

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“…Determining the causes/mechanisms of how mycotoxins induce detrimental effects on the reproduction system is important to avoid these adverse effects. The direct ovarian effects of β-zearalenol and HT-2 on pigs have been well documented [25][26][27]. Currently, little information is available regarding the effects of Fusarium mycotoxins on ovarian function in cows.…”

Section: Discussionmentioning

confidence: 99%

“…The potential effects of the zearalenone and T-2 toxins on the reproductive activity in pigs are related to the inhibitory effects on GC proliferation and steroidogenesis [26,31]. The high concentrations of ZEA could induce the apoptosis and impair the proliferation of porcine GCs in a dose-dependent manner [32].…”

Section: Discussionmentioning

confidence: 99%

Understanding the Toxin Effects of β-Zearalenol and HT-2 on Bovine Granulosa Cells Using iTRAQ-Based Proteomics

Yang

et al. 2020

Animals

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Zearalenone (ZEA) and T-2 are the most common mycotoxins in grains and can enter the animal and human food-chain and cause many health disorders. To elucidate the toxic response profile, we stimulated bovine granulosa cells (GCs) with β-zearalenol or HT-2. Using isobaric tags for relative and absolute quantification (iTRAQ)-based proteomic, 178 and 291 differentially expressed proteins (DEPs, fold change ≥ 1.3 and p-value < 0.05) in β-zearalenol and HT-2 groups were identified, respectively. Among these DEPs, there were 66 common DEPs between β-zearalenol and HT-2 groups. These 66 DEPs were associated with 23 biological processes terms, 14 molecular functions terms, and 19 cellular components terms. Most heat shock proteins (HSPs) were involved in the toxic response. Reactive oxygen species accumulation, the endoplasmic reticulum (ER)-stress related marker molecule (GRP78), and apoptosis were activated. β-zearalenol and HT-2 inhibited oestradiol (E2) production. These results emphasized the important function of HSPs, clarified oxidative stress, and demonstrated the caspase-3 signaling cascade involved in mycotoxin-treated toxic response, along with decreased E2 production. This study offers new insights into the toxicity of β-zearalenol and HT-2 on ovarian granulosa cells.

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“…There are only few studies on the interactions of mycotoxins in binary (Ficheux et al, 2012;Bensassi et al, 2014;Cortinovis et al, 2014) and especially in ternary mixtures (Kouadio et al, 2007;Wan et al, 2013a,b). Therefore, the aim of this study was to determine the concentration that inhibits cell viability by 50% (IC 50 values) of FB 1 , DON and ZEN and to investigate their combined cyto-and genotoxic effects exerted on porcine lymphocytes in low concentrations.…”

Section: Acta Veterinaria Hungarica 65 2017mentioning

confidence: 99%

Preliminary results on the interactive effects of deoxynivalenol, zearalenone and fumonisin B1 on porcine lymphocytes

Kachlek¹,

Szabó-Fodor²,

Bodnár

et al. 2017

Acta Veterinaria Hungarica

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Fusarium mycotoxins, such as fumonisin B 1 (FB 1 ), deoxynivalenol (DON) and zearalenone (ZEN), frequently co-occur in feed raw materials and their presence is ubiquitous. The aims of this study were to determine the concentration that inhibits cell viability by 50% (IC 50 values) for each mycotoxin (after 24, 48 and 72 h) and to investigate their combined effects in binary (DON + ZEN: DZ, DON + FB 1 : DF, FB 1 + ZEN: FZ) and ternary (DFZ) mixtures using cyto-and genotoxicity on porcine lymphocytes as endpoints. The potency of cytotoxicity of the three toxins in an increasing order was FB 1 < ZEN < DON. The range of IC values depending on the period of exposure was 0.31-0.42 μg/ml and 16.6-22.9 μg/ml for DON and ZEN, respectively, and 101.15 μg/ml for FB 1 (50% viability was reached only after 72 h). The main interaction observed was antagonism regarding cytotoxicity. Lower and higher sets of concentrations were used for the genotoxicity (comet assay) experiments. When lower concentrations were used, antagonism was again the main interaction observed. However, at higher concentrations an antagonism was confirmed only for DFZ, whereas for DZ and FZ a synergism was observed. Interactions of DF were inconsistent in different exposure periods in both series of experiments. Further studies with additional endpoints should be performed (e.g. DNA fragmentation, protein synthesis) in order to elucidate the mechanisms underlying the interactions observed.

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Effects of fumonisin B1 alone and combined with deoxynivalenol or zearalenone on porcine granulosa cell proliferation and steroid production

Cited by 70 publications

References 49 publications

Individual and combined effects of deoxynivalenol and α-zearalenol on cell proliferation and steroidogenesis of granulosa cells in cattle

Individual and combined effects of deoxynivalenol and α-zearalenol on cell proliferation and steroidogenesis of granulosa cells in cattle

Understanding the Toxin Effects of β-Zearalenol and HT-2 on Bovine Granulosa Cells Using iTRAQ-Based Proteomics

Preliminary results on the interactive effects of deoxynivalenol, zearalenone and fumonisin B1 on porcine lymphocytes

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