Effects of fullerenol C60(OH)24 on the frequency of micronuclei and chromosome aberrations in CHO-K1 cells

In vitro toxicity assessment of three hydroxylated fullerenes in human skin cells

“…Tsai demonstrated the free-radical scavenging activity of hydroxylated fullerenes which prevented hydrogen peroxide-elicited oxidative damage of brain tissue in rats (Tsai et al, 1997). Fullerenols have also been shown to reduce cardiotoxicity and modulate the cytotoxic effects associated with doxorubicin chemotherapy (Bogdanovic et al, 2004; Torres et al, 2010), and to mediate the decrease in the frequency of micronuclei and chromosome aberrations in hamster ovarian cells (Mrdanović et al, 2009). Additionally, fullerenols showed an increase in the cytoprotection of UV-induced oxidative damage proportional to increasing hydroxylation levels (Saitoh et al, 2011).…”

“…However, there is an increasing amount of literature demonstrating the cytotoxic effects of hydroxylated fullerenes (Isakovic et al, 2006; Johnson-Lyles et al, 2010; Jovanović et al, 2010; Mrdanović et al, 2009; Su et al, 2010; Usenko et al, 2007; Weilgus et al, 2010; Yamada et al, 2010). Hydroxylated fullerenes have been shown to significantly decrease cell viability and cause G1 phase cell cycle arrest in Chinese hamster ovarian and lung cells, but only a slight decrease in viability with no cell cycle effects in mouse fibrosarcoma cells (Su et al, 2010).…”

“…Hydroxylated fullerenes have been shown to significantly decrease cell viability and cause G1 phase cell cycle arrest in Chinese hamster ovarian and lung cells, but only a slight decrease in viability with no cell cycle effects in mouse fibrosarcoma cells (Su et al, 2010). Fullerenol toxicity was demonstrated in Chinese hamster ovarian cells with a lethal concentration (LC)50 found at 250μg/ml (Mrdanović et al, 2009), and also shown in rat astrocytes with an inhibitory concentration (IC)50 of fullerenol found at 1.74μg/ml coupled with decreased in vivo locomotor activity (Yamada et al, 2010). Hydroxylated fullerenes have been shown to induce cytoskeletal and mitochondrial aberrations in porcine renal tubule cells exposed to 5.8mg/ml (Johnson-Lyles et al, 2010).…”

Bioimpact of Carbon Nanomaterials

“…In addition, several studies confi rmed the absence of C 60 (OH) 24 genotoxicity towards DNA [369,370,78]. Mrd -anović et al [370] proved that fullerenol, C 60 (OH) 24 , leads to dose-dependent decrease in micronuclei incident and chromosome aberrations at subcytotoxic concentrations during 3-hour and 24-hour treatment of hamster ovary cell line CHO-K1 [370], and periferal blood primary lymphocyte culture throughout G0, G1/S, G1/S/G2/M phases [369].…”

“…In addition, several studies confi rmed the absence of C 60 (OH) 24 genotoxicity towards DNA [369,370,78]. Mrd -anović et al [370] proved that fullerenol, C 60 (OH) 24 , leads to dose-dependent decrease in micronuclei incident and chromosome aberrations at subcytotoxic concentrations during 3-hour and 24-hour treatment of hamster ovary cell line CHO-K1 [370], and periferal blood primary lymphocyte culture throughout G0, G1/S, G1/S/G2/M phases [369]. Furthermore, fullerenol effectively protected previously mentioned cell lines from chromosome aberrations caused by Mytomycine C. The DNA protective effect of fullerenol is attributed to its ROS scavenging potential that reduces the levels of intracellulary and extracellulary produced ROS [371].…”