Effects of fullerene C60 on liver tissue in liver ischemia reperfusion injury in rats undergoing sevoflurane anesthesia

Abstract

This study aimed to investigate the effects of fullerene C60 on rat liver tissue in a liver ischemia reperfusion injury (IRI) model under sevoflurane anesthesia to evaluate the ability of nanoparticles to prevent hepatic complications. A total of 36 adult female Wistar Albino rats were divided into six groups, each containing six groups as follows: sham group (Group S), fullerene C60 group (Group FC60), ischemia-reperfusion group (Group IR), ischemia-reperfusion-sevoflurane group (Group IR-Sevo), ischemia-reperfusion-fullerene C60 group (Group IR-FC60), and ischemia-reperfusion-fullerene C60-sevoflurane group (Group IR-FC60-Sevo). Fullerene C60 100 mg/kg was administered to IR-FC60 and IR-FC60-Sevo groups. In the IR group, 2 h of ischemia and 2 h of reperfusion were performed. At the end of reperfusion, liver tissues were removed for biochemical assays and histopathological examinations. Hepatocyte degeneration, sinusoidal dilatation, prenecrotic cells, and mononuclear cell infiltration in the parenchyma were significantly higher in Group IR than in all other groups. Thiobarbituric acid reactive substances levels were significantly higher in Group IR than in the other groups, and the lowest thiobarbituric acid reactive substances level was in Group IR-FC60 than in the other groups, except for Groups S and FC60. Catalase and Glutathione-S-transferase activities were reduced in the IR group compared to all other groups. Fullerene C60 had protective effects against liver IR injury in rats under sevoflurane anesthesia. The use of fullerene C60 could reduce the adverse effects of IRI and the associated costs of liver transplantation surgery.

С60 fullerene protective effect against the rat muscle soleus trauma

“…We have previously shown, that water-soluble C 60 fullerenes prevent the restraint of stress-induced oxidative disorders in rat' brain and heart tissues [ 8 ] as well as an acute liver injury [ 9 ], diminish muscle fatigue in rats [ 10 ], markedly decrease the level of oxidative stress in diet-induced obesity rats [ 11 ] and, finally, realize therapeutic effects in the models of muscle injury and ischemia of the muscle soleus in rats [ 12 , 13 ]. Recenly the authors [ 14 , 15 ] demonstrated the protective effect of C 60 fullerene on liver tissue in liver ischemia reperfusion injury as well as on lung and renal tissue in lower extremity ischemia-reperfusion injury in rats, which opens up a prospect for its use in biomedicine.…”