Effects of Freezing on the Viability of Nine Pathogens from Quarters with Subclinical Mastitis

Murdough, P.A.; Deitz, K.E.; Pankey, J.W.

doi:10.3168/jds.s0022-0302(96)76368-3

Cited by 45 publications

(36 citation statements)

References 4 publications

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“…TNase was confirmed to retain its 100% activity at these temperatures as was expected due to its thermal stability. These results are also consistent with other reports, that freezing of quarter milk samples for 6 weeks did not effect the viability of nine pathogens including S. aureus [19].…”

Section: Resultssupporting

confidence: 83%

Effect of preservation techniques and food additives on staphylococcal thermonuclease

Kumar

Sharma

Kulkarni³

2000

Nahrung

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Staphylococcal TNase was found to retain its activity fully even after exposure to chilling and refrigeration temperatures for 24 h. It was not inhibited by p-hydroxy benzoic acid, sorbic acid, methylpropyl p-benzoic acid and sodium nitrite in the concentration range of 0.04 to 0.5%, whereas it was inhibited by 100 ppm of butylated hydroxy anisole (BHA), 200 ppm of butylated hydroxy toluene (BHT), and 300 ppm of propyl gallate. There was not complete inhibition of S. aureus and TNase by tocopherol (TP) and ascorbic acid (AA) even at concentration of 300 ppm. These results indicate that TNase can be used as an index of potentially enterotoxin producing S. aureus contamination in foods subjected to chilling, refrigeration as well as in foods containing common preservatives and antioxidants.

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Section: Resultssupporting

confidence: 83%

Effect of preservation techniques and food additives on staphylococcal thermonuclease

Kumar

Sharma

Kulkarni³

2000

Nahrung

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“…aureus isolation. Murdough et al (1996) and McDougall (1999) found that pre-culture freezing at -20 8C had no effect on the viability of a range of bacterial species isolated from subclinically affected bovine and caprine milk samples. Schukken et al (1989) found that that pre-culture freezing at -20 8C, and increased duration of freezing, decreased the number of bovine milk samples from which Esch.…”

Section: Discussionmentioning

confidence: 97%

Evaluation of diagnostic procedures for subclinical mastitis in meat-producing sheep

Clements

Taylor

Fitzpatrick

2003

Journal of Dairy Research

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Samples of foremilk were collected from 261 clinically normal glands of 150 ewes, and tested using the California mastitis test (CMT). Further samples were collected from 195 of these glands for determination of automated somatic cell counts (SCC), and from 60 of these glands for bacteriological assessment. The sensitivity and specificity of CMT for detecting samples with SCC above different threshold levels and for CMT and SCC in determining bacteriological status were evaluated using two-graph receiver operating characteristics (TG-ROC). Milk samples were obtained subsequently from ten CMT positive, and five CMT negative first-and second-lactation ewes. Samples were cultured using a variety of media, incubation temperatures and atmospheric conditions, immediately after collection, and 1 week after storage at 4 8C and -21 8C. Results suggested that CMT is best used as a diagnostic test for ovine subclinical mastitis (SCM) with a cut-off of 3 (distinct gel formation), and that automated SCC thresholds of > 1200r10 3 cells/ml are appropriate, especially where low prevalences are expected (e.g. < 5%). Additionally, this study showed that routine bacteriological methods were appropriate for isolation of most species of pathogen responsible for ovine SCM, but storage of samples prior to culture, either at 4 8C or -21 8C, was detrimental to the isolation of several of these organisms.

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“…Numerous studies have shown that freezing has no effect on Staphylococcus aureus (Luedecke et al 1972;Murdough et al 1996). Several studies have determined that there is a significant decrease in the number of Eschrichia coli isolated in samples following freezing (Schukken et al 1989).…”

Section: Discussionmentioning

confidence: 97%

Effect of storage conditions on detection of mycoplasma in biopharmaceutical products

Cheng

Shen

Wang

2007

In Vitro Cell.Dev.Biol.-Animal

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Mycoplasma contamination affects many different aspects of cell culturing, resulting in unreliable experimental results and potentially harmful biological products. Therefore, the specificity, sensitivity, and reliability of detecting mycoplasma contamination are important aspects of quality control in biotechnological products. In this study, Mycoplasma hyorhinis was adopted as a model strain to evaluate the effects of storage on the viability of Mycoplasma species in cell culture samples. Medium X was compared with conventional media 243 and 988 for the ability to detect M. hyorhinis. The 10(1) CFU/ml of M. hyorhinis was inoculated into medium X prepared using the same lots of components and preserved for 7 d, 1 mo, and 2 mo. M. hyorhinis grew readily and typically on agar plates prepared within 1 mo. The viable mycoplasmas in samples containing different initial titers (10(1) and 10(6) CFU/ml) after storage at 4 degrees C and -30 degrees C were analyzed. During storage, viable organisms were found with little or no reduction in titers after storage for 8 wk at -30 degrees C under aerobic and anaerobic conditions. A reduction in titers of 3 log10 occurred after 4 wk storage for high-dose cultures (10(6) CFU/ml) at 4 degrees C. The titers of viable organisms were diminished over 8 wk at 4 degrees C under aerobic and anaerobic conditions.

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Effects of Freezing on the Viability of Nine Pathogens from Quarters with Subclinical Mastitis

Cited by 45 publications

References 4 publications

Effect of preservation techniques and food additives on staphylococcal thermonuclease

Effect of preservation techniques and food additives on staphylococcal thermonuclease

Evaluation of diagnostic procedures for subclinical mastitis in meat-producing sheep

Effect of storage conditions on detection of mycoplasma in biopharmaceutical products

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