Effects of freezing and storage temperature on MS2 viability

Olson, Matthew R.; Axler, Richard P; Hicks, Randall E.

doi:10.1016/j.jviromet.2004.08.010

Cited by 111 publications

(87 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This could be attributed to the phages being able to acclimatize to the incubation temperature and conditioned to optimum bacteriolytc activity before the bacterial culture was added (Taj et al, 2014). Temperature plays key roles in the attachment, adsorption, latent period, and multiplication of phages, and thus could greatly affect their infectivity (Olson et al, 2004; Jończyk et al, 2011). Efficacy was not as significant in Sets 2 and 4, where the phages were added directly from storage (at 4°C) to the culture at 37°C.…”

Section: Discussionmentioning

confidence: 99%

‘Get in Early’; Biofilm and Wax Moth (Galleria mellonella) Models Reveal New Insights into the Therapeutic Potential of Clostridium difficile Bacteriophages

et al. 2016

View full text Add to dashboard Cite

Clostridium difficile infection (CDI) is a global health threat associated with high rates of morbidity and mortality. Conventional antibiotic CDI therapy can result in treatment failure and recurrent infection. C. difficile produces biofilms which contribute to its virulence and impair antimicrobial activity. Some bacteriophages (phages) can penetrate biofilms and thus could be developed to either replace or supplement antibiotics. Here, we determined the impact of a previously optimized 4-phage cocktail on C. difficile ribotype 014/020 biofilms, and additionally as adjunct to vancomycin treatment in Galleria mellonella larva CDI model. The phages were applied before or after biofilm establishment in vitro, and the impact was analyzed according to turbidity, viability counts and topography as observed using scanning electron and confocal microscopy. The infectivity profiles and efficacies of orally administered phages and/or vancomycin were ascertained by monitoring colonization levels and larval survival rates. Phages prevented biofilm formation, and penetrated established biofilms. A single phage application reduced colonization causing extended longevity in the remedial treatment and prevented disease in the prophylaxis group. Multiple phage doses significantly improved the larval remedial regimen, and this treatment is comparable to vancomycin and the combined treatments. Taken together, our data suggest that the phages significantly reduce C. difficile biofilms, and prevent colonization in the G. mellonella model when used alone or in combination with vancomycin. The phages appear to be highly promising therapeutics in the targeted eradication of CDI and the use of these models has revealed that prophylactic use could be a propitious therapeutic option.

show abstract

Section: Discussionmentioning

confidence: 99%

‘Get in Early’; Biofilm and Wax Moth (Galleria mellonella) Models Reveal New Insights into the Therapeutic Potential of Clostridium difficile Bacteriophages

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Wen's conclusions are coherent with those of Brussaard (2004), who uses flow cytometry combined with liquid nitrogen, deep freezing (-80ºC), and glutaraldehyde. In contrast, other authors have reached the conclusion that storing at -80ºC is more effective only when the objective is to keep the samples for long periods of time (more than 40 days) but that for shorter periods storing at 4ºC is better (Olson et al, 2004). It is important to note that methodological controversies are frequent in the fields of marine microbiology and biological oceanography.…”

Section: Discussionmentioning

confidence: 97%

Relationship between viral and prokaryotic abundance on the Bajo O’Higgins 1 Seamount (Humboldt Current System off Chile)

Quiñones¹

2007

Sci. Mar.

View full text Add to dashboard Cite

SUMMARY: There is little known about the ecology of microbial communities living in the water column over seamounts. Here, for the first time, the spatial distribution and abundance of virus-like particles (VLP) are described over a seamount. The association between VLP distribution, prokaryotic abundance, and environmental variables is also analyzed. Sampling was conducted in December 2004 on the Bajo O'Higgins 1 seamount (32°54'S, 73°53'W) located in the Humboldt Current System off Chile. A oxygen minimum layer (OMZ) was clearly present between 130 and 280 m in the water column over the seamount. Water samples were taken with Niskin bottles at 10 oceanographic stations over the seamount at depths of 5, 20, 50, 75, 100, and 150 m and at the benthic boundary layer (BBL; 5-12 m over the sediments). Temperature, salinity, oxygen, chlorophyll a, and phaeopigments were measured at each station. Viral and prokaryotic abundances were determined with fluorochrome SYBR Green I. Viral abundance ranged from 1.53 x 10 9 VLP L -1 -16.48 x 10 9 VLP L -1 , whereas prokaryotic abundance ranged from 1.78 x 10 8 cell L -1 -14.91 x 10 8 cell L -1 . The virus-like particle/prokaryote ratio varied widely among the analyzed layers (i.e. surface, OMZ, and BBL), probably due to the presence of different prokaryotic and viral assemblages in each layer. Our results indicate that the environmental conditions, mainly the concentration of dissolved oxygen in the water column over Bajo O'Higgins 1 seamount, shape the association between viral and prokaryotic abundance.Keywords: virus-like particles, prokaryotes, seamount, oxygen minimum zone, benthic boundary layer, Humboldt Current System. RESUMEN: RELACIÓN ENTRE LA ABUNDANCIA VIRAL Y PROCARIÓTICA SOBRE EL MONTE SUBMARINO BAJO O'HIGGINS 1 (SISTEMA DE LA CORRIENTE DE HUMBOLDT FRENTE A CHILE). -La ecología de comunidades microbianas que habitan en la columna de agua sobre montes submarinos es escasamente conocida. Aquí, por primera vez, se describe la distribución y abundancia de partículas virales (VLP) sobre un monte submarino. La asociación entre la distribución de VLP, abundancia de procariotas y variables ambientales es también analizada. El muestreo fue realizado en Diciembre del 2004 sobre el monte submarino Bajo O'Higgins 1 (32°54'S, 73°53'W) localizado en el Sistema de la Corriente de Humboldt frente a Chile. Una zona de mínimo oxígeno se detectó entre 130 y 280 m en la columna de agua sobre el monte submarino. Las muestras de agua fueron tomadas con botellas Niskin desde 10 estaciones oceanográficas a 5, 20, 50, 75, 100, y 150 m de profundidad y en la capa de interfase bentónica (BBL; 5-12 m sobre el sedimento). Las variables temperatura, salinidad, oxígeno, clorofila a y feopigmentos fueron medidas en cada estación. La abundancia de virus y procariotas se determinó utilizando el fluorocromo SYBR Green I. La abundancia viral varió entre 1.53 x 10 9 VLP L -1 -16.48 x 10 9 VLP L -1 , mientras que la abundancia de células procariotas lo hizo entre 1.78 x 10 8 células L -1 -14.91 x 10 ...

show abstract

“…These observations likely refl ect mechanical damage caused by the formation of ice crystals during freezing at this temperature. A study performed by Olson and colleagues 20 demonstrated increased viral loss at -20ºC, emphasizing that viral samples should not be stored at -20ºC because of ice crystal formation and the consequent possibility of damage to the viral structure.…”

Section: Discussionmentioning

confidence: 99%