Effects of Free Fatty Acids with Different Chain Lengths and Degrees of Saturability on the Milk Fat Synthesis in Primary Cultured Bovine Mammary Epithelial Cells

Yan, Qiongxian; Tang, Shaoxun; Zhou, Chuanshe; Han, Xuefeng; Tan, Zhiliang

doi:10.1021/acs.jafc.9b02905

Cited by 15 publications

(10 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Non-esterified fatty acid (NEFA) is a source of fatty acids and can increase milk fat synthesis [ 8 ]. A variety of genes, such as fatty acid synthase ( FASN ), acetyl coenzyme-A carboxylase 1 ( ACACA ), and stearoyl-CoA desaturase ( SCD ), etc., are involved in milk fat synthesis [ 9 , 10 , 11 , 12 , 13 , 14 ]. FASN catalyzes the synthesis of long-chain fatty acids [ 15 ], ACACA is the rate-limiting enzyme catalyzing the first reaction step of fatty acid synthesis [ 16 ], and SCD is responsible for catalyzing the synthesis of mono-saturated fatty acids [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Peptidoglycan, Lipoteichoic Acid and Lipopolysaccharide on Inflammation, Proliferation and Milk Fat Synthesis in Bovine Mammary Epithelial Cells

Sun

Zhu

et al. 2020

Toxins

View full text Add to dashboard Cite

The mammary gland of the cow is particularly susceptible to infections of a wide range of pathogenic bacteria, including both Gram-positive and Gram-negative bacteria. The endotoxins of these pathogenic bacteria include peptidoglycan (PGN), lipoteichoic acid (LTA) and lipopolysaccharide (LPS), and they are the pathogen-associated molecular patterns (PAMPs) to induce mastitis. LPS can directly inhibit proliferation and milk fat synthesis of bovine mammary epithelial cells (BMECs) while inducing mastitis, but it is unclear whether PGN and LTA also have such effects. Furthermore, since the three PAMPs usually appear simultaneously in the udder of cows with mastitis, their synergistic effects on proliferation and milk fat synthesis of BMECs are worth investigating. The immortalized BMECs (MAC-T cells) were stimulated for 24 h using various concentrations of PGN, LTA and LPS, respectively, to determine the doses that could effectively cause inflammatory responses. Next, the cells were stimulated for 24 h with no endotoxins (CON), PGN, LTA, LPS, PGN + LTA, and PGN + LTA + LPS, respectively, with the predetermined doses to analyze their effects on proliferation and milk fat synthesis of BMECs. PGN, LTA and LPS successfully induced inflammatory responses of BMECs with doses of 30, 30 and 0.1 μg/mL, respectively. Although the proliferation of BMECs was significantly inhibited in the following order: LTA < PGN + LTA < PGN + LTA + LPS, there was no change in cell morphology and cell death. LTA significantly promoted the expression of fatty acid synthesis-related genes but did not change the content of intracellular triglyceride (TG), compared with the CON group. The mRNA expression of fatty acid synthesis-related genes in the LPS group was the lowest among all the groups. Meanwhile, LPS significantly decreased the content of intracellular non-esterified fatty acids (NEFAs) and TG, compared with the CON group. PGN had no effects on milk fat synthesis. Co-stimulation with PGN, LTA and LPS significantly increased the expression of fat acid synthesis-related genes and the intracellular NEFAs, but decreased intracellular TG, compared with sole LPS stimulation. Collectively, PGN, LTA and LPS showed an additive effect on inhibiting proliferation of BMECs. The promoting role of LTA in fatty acid synthesis might offset the negative effects of LPS in this regard, but co-stimulation with PGN, LTA and LPS significantly decreased intracellular TG content.

show abstract

Section: Introductionmentioning

confidence: 99%

Effects of Peptidoglycan, Lipoteichoic Acid and Lipopolysaccharide on Inflammation, Proliferation and Milk Fat Synthesis in Bovine Mammary Epithelial Cells

Sun

Zhu

et al. 2020

Toxins

View full text Add to dashboard Cite

show abstract

“…Sodium acetate is one of the most important precursors of fatty acid synthesis in cell [6,22,23]. Studies have shown that the milk fat synthesis of mammary gland cells in vitro can be promoted by the addition of sodium acetate in medium [7,24,25].…”

Section: Discussionmentioning

confidence: 99%

“…The synthesis of milk fat is controlled by genetics, hormones, nutrition and environment [2][3][4][5]. Short-chain fatty acids such as sodium acetate, sodium β-hydroxybutyrate, are important precursors for the synthesis of fatty acids, and they are also important for the synthesis of milk fat in bovine mammary epithelial cells (BMECs) [6,7]. The sterol regulatory element binding protein 1 (SREBP1) pathway is one of the most important pathways that regulate milk fat synthesis in BMECs [8,9].…”

Section: Introductionmentioning

confidence: 99%

TATA Element Modulatory Factor 1 Negatively Regulates Sodium Acetate Activated Milk Fat Synthesis Through SREBP1 Pathway in Bovine Mammary Epithelial Cells

Wang

2021

Preprint

View full text Add to dashboard Cite

Background: Sodium acetate is one of the important nutrients that regulate milk fat synthesis in bovine mammary epithelial cells (BMECs), and it regulates milk fat synthesis mainly through the SREBP1 pathway. Our previous study has showed that TATA element modulatory factor 1 (TMF1) may be interacts with SREBP1 and regulates the sodium acetate-dependent milk synthesis in BMECs, but the underlying mechanism is unclear. In the current study, the effect of TMF1 on sodium acetate activated milk fat synthesis in BMECs was assessed. Results: Overexpressing or inhibiting TMF1 demonstrated that TMF1 negatively regulated sodium acetate activated sterol regulatory element-binding protein 1 (SREBP1) pathway and milk fat synthesis; Overexpressing or inhibiting SREBP1 showed that TMF1 inhibited sodium acetate activated milk fat synthesis through SREBP1 pathway; Co-immunoprecipitation analysis showed that TMF1 interacted with SREBP1; Nuclear localization of SREBP1 analysis showed that sodium acetate activated nuclear localization of SREBP1 was inhibited by TMF1; Depletion or supply sodium acetate demonstrated that sodium acetate negatively regulated expression of TMF1 and the interaction between TMF1 and SREBP1. Conclusions: Together, these results indicate that TMF1 is a negative regulatory factor for sodium acetate activated milk fat synthesis, it induced expression by sodium acetate depletion, and interacts with SREBP1 in cytoplasmic, prevents the nuclear localization of SREBP1 and then suppresses the expression of SREBP1 target gene and subsequent milk fat synthesis in BMECs.

show abstract

“…Then, 400 μL of CHCl 3 was added. After vortexing for 5 min, the mixture was centrifuged for 10 min at 10000 rpm, and all of the chloroform layer was diluted 10‐fold with ACN [39].…”

Section: Methodsmentioning

confidence: 99%

Determination of trans‐fatty acids in food samples based on the precolumn fluorescence derivatization by high performance liquid chromatography

Wang

Guo

Fan

et al. 2022

J of Separation Science

View full text Add to dashboard Cite

Trans-fatty acids are unsaturated fatty acids that are considered to have health risks. 1,3,5,7-Tetramethyl-8-butyrethylenediamine-difluoroboradiaza-s-indacene is a highly sensitive fluorescent labeling reagent for carboxylic acids developed by our lab. In this study, using this precolumn fluorescent derivatization reagent, a rapid and accurate high-performance liquid chromatography with fluorescence detection method was developed for the determination of two trans-fatty acids in food samples. Under the optimized derivative conditions, two trans-fatty acids were tagged with the fluorescent labeling reagent in the presence of 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide at 25 • C for 30 min. Then, the baseline separation of trans-and cis-fatty acids and their saturated fatty acid with similar structures was achieved with less interference using a reversed-phased C 18 column with isocratic elution in 14 min. With fluorescence detection at λ ex /λ em = 490 /510 nm, the linear range of the TFAs was 1.0-200 nM with low detection limits in the range of 0.1-0.2 nM (signal-to-noise ratio = 3). In addition, the proposed approach was successfully applied for the detection of trans-fatty acids in food samples, and the recoveries using this method ranged from 96.02 to 109.22% with low relative standard deviations of 1.2-4.3% (n = 6).

show abstract

Effects of Free Fatty Acids with Different Chain Lengths and Degrees of Saturability on the Milk Fat Synthesis in Primary Cultured Bovine Mammary Epithelial Cells

Cited by 15 publications

References 45 publications

Effects of Peptidoglycan, Lipoteichoic Acid and Lipopolysaccharide on Inflammation, Proliferation and Milk Fat Synthesis in Bovine Mammary Epithelial Cells

Effects of Peptidoglycan, Lipoteichoic Acid and Lipopolysaccharide on Inflammation, Proliferation and Milk Fat Synthesis in Bovine Mammary Epithelial Cells

TATA Element Modulatory Factor 1 Negatively Regulates Sodium Acetate Activated Milk Fat Synthesis Through SREBP1 Pathway in Bovine Mammary Epithelial Cells

Determination of trans‐fatty acids in food samples based on the precolumn fluorescence derivatization by high performance liquid chromatography

Contact Info

Product

Resources

About