Effects of forskolin and phorbol-myristate-acetate on cytoskeleton, extracellular matrix and protein phosphorylation in human endothelial cells

Смирнов, В. Н.; Antonov, Alexander S.; Antonova, Galina; Romanov, Yu. A.; Kabaeva, N. V.; Tchertikhina, Irene V.; Lukashev, Matvey

doi:10.1016/0022-2828(89)90832-8

Cited by 16 publications

(5 citation statements)

References 13 publications

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“…Numerous descriptions of nerve endings on cerebral vasculature have been provided (Harik et al, 1981;Goldstein and Betz, 1986), and neurotransmitter-target cell interactions might involve changes in second messengers . It has also been observed that second messengers cause some changes in epithelial and peripheral EC permeability (Duffey et al, 1981;Smirnov et al, 1989;Stelzner et al, 1989;Balda et al, 1990;Yamada et al, 1990), so we investigated the effects of second messenger manipulation on brain EC morphology and resistance.…”

Section: Effect Ofincreased Camplevels On the Distribution Of Junctiomentioning

confidence: 98%

A cell culture model of the blood-brain barrier.

Rubin

Hall

Porter

et al. 1991

The Journal of Cell Biology

696

403

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Abstract. Endothelial cells that make up brain capillaries and constitute the blood-brain barrier become different from peripheral endothelial cells in response to inductive factors found in the nervous system . We have established a cell culture model of the bloodbrain barrier by treating brain endothelial cells with a combination of astrocyte-conditioned medium and agents that elevate intracellular cAMP These cells form

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Section: Effect Ofincreased Camplevels On the Distribution Of Junctiomentioning

confidence: 98%

A cell culture model of the blood-brain barrier.

Rubin

Hall

Porter

et al. 1991

The Journal of Cell Biology

696

403

View full text Add to dashboard Cite

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“…Activation of PKC is known to affect multiple cardiovascular functions, including vascular permeability, cell migration and growth [234,235]; extracellular matrix production [236][237][238] and expression of various cytokines [239,240]; ion conductance and transport activity [241]; intracellular calcium homeostasis and properties of contractile proteins [242]; ischemic preconditioning of the heart [243]; genesis of arrhythmias [244]; and induction of cardiac hypertrophy [245,246]. The three groups of the PKC family of kinases comprise approximately 13 different isozymes [conventional (PKCα, PKCβ I, PKCβ II, PKCγ), novel (PKCδ, PKCε, PKCθ, PKCη, PKCμ) and atypical (PKCζ, PKCι, PKCν, PKCλ)].…”

Section: Enzymes With Protein Kinase Activitiesmentioning

confidence: 99%

Modulation of enzymatic activities by n-3 polyunsaturated fatty acids to support cardiovascular health

Siddiqui

Harvey

Zaloga

2008

The Journal of Nutritional Biochemistry

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“…We have observed that CAMP levels of SMC preincubated with PMA prior to isoproterenol or 5-HT plus IBMX remain threeto fourfold elevated (unpublished data). Antonov et al (1986) and Smirnov et al (1989) reported that PMA itself did not produce a cellular shape change of EC, but enhanced the effect of forskolin, which al- tered EC morphology through phosphorylation of intermediate filament protein.…”

Section: -Ht or Isoproterenol And The Configurational Changementioning

confidence: 99%

Serotonin produces a configurational change of cultured smooth muscle cells that is associated with elevation of intracellular cAMP

Lee

Fanburg

1992

Journal Cellular Physiology

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Early passaged bovine pulmonary artery smooth muscle cells (SMC) respond to serotonin (5-HT) by developing a reversible change in configuration. (Lee et al. J. Cell. Physiol. 138:145, 1989). This configurational change does not occur in pulmonary artery endothelial cells (EC) subjected to 5-HT and is adenosine triphosphate (ATP) dependent, lost with passage of SMC, and inhibited by various agents that block high-affinity 5-HT uptake. We now report a second configurational change (also dendritic formation) of SMC produced by 5-HT only in the presence of isobutylmethylxanthine (IBMX), an inhibitor of phosphodiesterase. This configurational change was also ATP dependent, but unlike the first response, (Lee et al., 1989), it occurred in both first and later passaged SMC and was not inhibited by blockade of 5-HT uptake. Also, unlike the response with 5-HT alone that failed to elevate cAMP, this one was associated with a large elevation of cAMP (eight fold above control values), similar to the response to the beta-agonist isoproterenol, plus IBMX. The second response was not blocked by a variety of 5-HT receptor antagonists but was reproduced by (+/-)-8-hydroxy-DPAT HBr (8-OH-DPAT), a reputed 5-HT1A agonist. The response was not dependent upon Ca2+ and was blocked by 1-2 mM n-phenylanthranilic acid or anthracene-9-carboxylic acid, electrically conductive Cl- channel inhibitors. Hence, 5-HT in the presence of IBMX causes a marked elevation of cAMP of SMC and this elevation in cAMP likely results in a cellular configurational change through a Cl- channel-dependent mechanism similar to that we previously described for EC in the presence of beta-adrenergic agonist stimulation (Ueda et al. Circ. Res. 66:951, 1990). EC do not show a similar response to 5-HT possibly because cAMP is not adequately elevated, even in the presence of IBMX, to enhance Cl- channel activity. We propose that our observations indicate the presence of two sites of action of 5-HT on the smooth muscle cell, one intracellularly and another at a cell surface receptor.

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Effects of forskolin and phorbol-myristate-acetate on cytoskeleton, extracellular matrix and protein phosphorylation in human endothelial cells

Cited by 16 publications

References 13 publications

A cell culture model of the blood-brain barrier.

A cell culture model of the blood-brain barrier.

Modulation of enzymatic activities by n-3 polyunsaturated fatty acids to support cardiovascular health

Serotonin produces a configurational change of cultured smooth muscle cells that is associated with elevation of intracellular cAMP

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