Effects of food on bacterial community composition associated with the copepod
            <i>Acartia tonsa</i>
            Dana

Tang, Kam W.; Dziallas, Claudia; Hutalle-Schmelzer, Kristine Michelle L.; Grossart, Hans‐Peter

doi:10.1098/rsbl.2009.0076

Cited by 47 publications

(49 citation statements)

References 26 publications

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“…(4) Several phylotypes are related with chitindegrading bacteria. Tang et al (2009a) postulated that the life strategies of the different copepod genera with regard to feeding strategies play an important role for the composition of the associated bacterial communities. Interestingly, we could not support their hypothesis, since the different copepod genera investigated in this study display different life styles (herbivorous, omnivorous or detrivorous), but did not show significant differences in their bacterial community in respect to DGGE patterns.…”

Section: Discussionmentioning

confidence: 99%

The microbiome of North Sea copepods

et al. 2013

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Copepods can be associated with different kinds and different numbers of bacteria. This was already shown in the past with culture-dependent microbial methods or microscopy and more recently by using molecular tools. In our present study, we investigated the bacterial community of four frequently occurring copepod species, Acartia sp., Temora longicornis, Centropages sp. and Calanus helgolandicus from Helgoland Roads (North Sea) over a period of 2 years using DGGE (denaturing gradient gel electrophoresis) and subsequent sequencing of 16S-rDNA fragments. To complement the PCR-DGGE analyses, clone libraries of copepod samples from June 2007 to 208 were generated. Based on the DGGE banding patterns of the two years survey, we found no significant differences between the communities of distinct copepod species, nor did we find any seasonality. Overall, we identified 67 phylotypes ([97 % similarity) falling into the bacterial phyla of Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria. The most abundant phylotypes were affiliated to the Alphaproteobacteria. In comparison with PCR-DGGE and clone libraries, phylotypes of the Gammaproteobacteria dominated the clone libraries, whereas Alphaproteobacteria were most abundant in the PCR-DGGE analyses.

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Section: Discussionmentioning

confidence: 99%

The microbiome of North Sea copepods

et al. 2013

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“…Methods used to quantify bacteria are also included (Table continued on next page) Alpha-, Beta-, and Gammaproteobacteria). Using the same technique, Tang et al (2009b) identified 37 discrete phylogenetic units from the marine copepod Acartia tonsa that belonged to 3 major bacteria groups (Alpha-and Gammaproteobacteria, Bacteroidetes). Surprisingly, Møller et al (2007) found only 3 different Roseobacter species on Calanus spp.…”

Section: ± 210mentioning

confidence: 99%

“…When the copepod Acartia tonsa was feeding on different axenic phytoplankton diets, the bacterial diversity associated with the copepod decreased and converged, indicating the presence of a rather stable resident bacterial community (Tang et al 2009b). In contrast, the bacterial diversity greatly diverged and several species such as Pseudoalteromonas, Sulfitobacter, and Roseobacter only appeared when the copepod was feeding on xenic phytoplankton diets, indicating that many of the transient bacteria either actively attached to the animal's body surfaces or were passively ingested (Tang et al 2009b). Similarly, in a field study, Grossart et al (2009) observed that the bacterial diversity associated with freshwater zooplankton (Thermocyclops oithonoides and Bosmina coregoni) decreased after gut evacuation.…”

Section: ± 210mentioning

confidence: 99%

Linkage between crustacean zooplankton and aquatic bacteria

Tang¹,

Turk²,

Grossart³

2010

Aquat. Microb. Ecol.

130

163

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Bacteria and metazoan zooplankton (mainly crustaceans) are often viewed as 2 separate functional groups in the pelagic food webs indirectly linked via nutrient cycling and trophic cascades. Yet a zooplankter's body carries a high abundance of diverse bacteria, often at an equivalent concentration orders of magnitude higher than the ambient bacterial concentration. Zooplankton bodies are organic-rich micro-environments that support fast bacterial growth. Their physical-chemical conditions differ from those in the surrounding water and therefore select for different bacterial communities, including anaerobic bacteria that otherwise may not thrive in a well-oxygenated water column. The zooplankton body provides protection to the associated bacteria from environmental stresses similar to biofilms. Furthermore, migration by zooplankton enables rapid dispersal of bacteria over vast distances and across boundaries such as the pycnocline. In addition to live zooplankton, molts, fecal pellets, and carcasses of zooplankton all influence water column and benthic microbial communities in various ways. We review the recent advances in the study of (crustacean) zooplankton-bacteria interactions and discuss future research opportunities and challenges. Traditional aquatic microbial ecology emphasizes free-living bacteria, which represent only a fraction of the microbial world. By transcending disciplinary boundaries, microbial ecologists and zooplankton ecologists can work together to integrate the two disciplines and advance our understanding in aquatic microbial ecology.

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“…Often the first line of defense for many groups of invertebrates, the hard, chitinous exoskeleton provides a physical and chemical barrier against pathogen attachment and invasion (78,79). A potentially vulnerable point of entry into the host, the gut is lined with the peritrophic matrix, which acts like a sieve that surrounds and prevents bacteria, bacterial toxins, and hard food fragments from contacting the intestinal epithelium (11,(80)(81)(82). When the thickness and permeability of the peritrophic matrix is compromised in Drosophila, there is higher susceptibility to infection by pathogenic bacteria or mortality from bacterial toxins (83).…”

Section: Host Regulation Of the Microbiomementioning

confidence: 99%

Interactions between calanoid copepod hosts and their associated microbiota

Almada¹

2015

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Zooplankton, such as copepods, are highly abundant environmental reservoirs of many bacterial pathogens. Although copepods are known to support diverse and productive bacterial communities, little is understood about whether copepods are affected by bacterial attachment and whether they can regulate these associations through mechanisms such as the innate immune response. This thesis investigates the potential role that copepod physiology may play in regulating Vibrio association and the community structure of its microbiome. To this end, the intrinsic ability of oceanic copepod hosts to transcriptionally respond to mild stressors was first investigated. Specifically, the transcriptional regulation of several heat shock proteins (Hsps), a highly conserved superfamily of molecular chaperones, in the copepod Calanus finmarchicus was examined and demonstrated that Hsps are a conserved element of the copepod's transcriptional response to stressful conditions and diapause regulation. To then investigate whether copepod hosts respond to and regulate their microbiota, the transcriptomic response of an estuarine copepod Eurytemora affinis to two distinct Vibrio species, a free-living strain (V. ordalii 12B09) and a zooplankton specialist (V. sp. F10 9ZB36), was examined with RNA-Seq. Our findings provide evidence that the copepod E. affinis does distinctly recognize and respond to colonizing vibrios via transcriptional regulation of innate immune response elements and transcripts involved in maintaining cuticle integrity. Our work also suggests that association with E. affinis can significantly impact the physiology of Vibrio colonists. Finally, the interindividual variability of the C. finmarchicus microbiome was examined to identify how specifically and predictably bacterial communities assemble on copepods and whether host physiology influences the bacterial community structure. Our findings suggest that copepods have a predictable "core microbiome" that persists throughout the host's entrance into diapause, a dormancy period characterized by dramatic physiological changes in the host. However, diapausing and active populations harbor distinct flexible microbiomes which may be driven by factors such including the copepod's feeding history, body size, and bacterial interactions. This thesis work highlights the role of copepods as dynamic reservoirs of diverse bacterial communities and implicates copepod host physiology as an important contributor to the activity, abundance, and community structure of its microbiome.

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Effects of food on bacterial community composition associated with the copepod Acartia tonsa Dana

Cited by 47 publications

References 26 publications

The microbiome of North Sea copepods

The microbiome of North Sea copepods

Linkage between crustacean zooplankton and aquatic bacteria

Interactions between calanoid copepod hosts and their associated microbiota

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