Effects of follicular phase and oocyte–cumulus complexes quality on the protein profile and in vitro oocyte meiosis competence in Cebus apella

Domingues, Sheyla Farhayldes Souza; Caldas-Bussiere, M.C.; Petretski, Marı́lvia D.A; Ohashi, Otávio Mitio; Lima, Joelson Sousa; Santos, Regiane R.; Cordeiro, M. S.; Castro, Paulo Henrique Gomes de

doi:10.1016/j.fertnstert.2009.05.029

Cited by 8 publications

(17 citation statements)

References 24 publications

(29 reference statements)

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“…Biopsies from each ovary were divided into 9 ovarian cortical pieces of 1 mm 3 . On completion of the procedure, 1 ovarian fragment (control) was immediately divided into 2 pieces, which were subjected to follicular isolation and viability analysis, or snap frozen in liquid nitrogen and stored at À80 C until RNA extraction.…”

Section: Experimental Designmentioning

confidence: 99%

Short-Term Culture of Ovarian Cortical Strips From Capuchin Monkeys (Sapajus apella): A Morphological, Viability, and Molecular Study of Preantral Follicular Development In Vitro

et al. 2013

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The aim of this study was to evaluate whether an in vitro culture (IVC) medium containing either or not b-mercaptoethanol (BME), bone morphogenetic protein 4 (BMP4), or pregnant mare serum gonadotrophin (PMSG) could be able to promote the development of capuchin monkeys' preantral follicles enclosed in ovarian cortical strips. Follicular viability after IVC was similar to control (89.32%). Primordial follicle recruitment to primary stage was not reached with IVC, but the rate of secondary follicle formation was increased in the medium supplemented with BME, BMP4, and PMSG (44.86%) when compared to IVC control (9.20%). In the medium supplemented with BME, BMP4, and PMSG, contrary to other media, anti-müllerian hormone-messenger RNA (mRNA) expression in ovarian tissue was upregulated (3.4-fold), while that of growth differentiation factor-9 was maintained. The BMP4-mRNA expression, however, appeared downregulated in all cultured tissues. Our findings show a favorable effect of BME, BMP4, and PMSG on the in vitro development of secondary follicles from capuchin monkeys.

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Section: Experimental Designmentioning

confidence: 99%

Short-Term Culture of Ovarian Cortical Strips From Capuchin Monkeys (Sapajus apella): A Morphological, Viability, and Molecular Study of Preantral Follicular Development In Vitro

et al. 2013

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“…The competence of oocytes retrieved from antral follicles in capuchin monkeys was achieved after 36 hours of IVM, the highest maturation rate occurring in oocytes collected from dominant follicles [71]. It has also demonstrated that the IVC of ovarian cortical strips in TCM199 supplemented of β-mercaptoethanol (BME), bone morphogenetic protein 4 (BMP4), or pregnant mare serum gonadotrophin (PMSG) promoted a follicular viability similar to that of controls (89.3%) for this species, while it also increases the rate of secondary follicle formation (44.9%) [70].…”

Section: In Vitro Culture and Maturation Of Oocytesmentioning

confidence: 99%

“…The recovery of oocytes by mechanical preantral follicle (PF) isolation in ovariectomy specimens [69], the collection of ovarian biopsies by exploratory laparoscopy [70], and the puncture of antral follicles by laparotomy [71] have been described studies in capuchin monkeys (Sapajus apella). In marmoset monkeys, oocyte recovery was achieved through follicle aspiration after laparotomy [72], laparoscopy [65], or uni-or bilateral ovariectomy [73].…”

Section: Recovery Of Female Gametesmentioning

confidence: 99%

“…Currently, in vitro embryo production depends primarily on two major products: meiotic viable oocytes (meiotic competence) and capacitated viable spermatozoa (sperm competence). Domingues et al [71] reported the viability of in vitro matured oocytes collected from nonstimulated capuchin monkeys. Lima et al [109] established 40 hours as the optimal IVM length and showed the positive effect of FSH/LH (0.5 µg/mL/50.0 µg/mL) on IVF of oocytes collected also in nonstimulated females.…”

Section: In Vitro Embryo Productionmentioning

confidence: 99%

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Assisted Reproductive Techniques on South American Wild Mammals

Silva¹,

Pereira²,

Lima³

et al. 2016

Insights From Animal Reproduction

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For South American wild mammals, assisted reproductive techniques (ARTs) have been established in accordance with the procedures used for domestic mammals. However, often, it is not possible to infer the experimental conditions from one species to another. In general, the use of these ARTs aims the conservation of the animal genetic material, using gamete manipulation, embryo technology by in vivo or in vitro methods, and cloning. In all animals, the previous knowledge of reproductive physiology becomes the starting point for the use of ARTs in the expansion of all species of interest. In this sense, the purpose of this chapter is to develop an approach of theoretical, technical, and applied aspects of the ARTs in South American wild mammals, with emphasis on the state-of-theart and its progress and perspectives.

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“…However, there is a paucity of efficient cryopreservation protocols for primordial follicles enclosed in the ovarian tissue from non-human primates (NHP), in special New World primates. Furthermore, ovarian biopsy may be performed by laparoscopy simultaneously with antral follicles puncture, under ultrasound monitoring (Domingues et al, 2010). As well, protocols developed using human ovarian tissue should be tested in NHP species before application.…”

Section: Introductionmentioning

confidence: 99%

Adaptation of a trap door technique for the recovery of ovarian cortical biopsies from Cebus apella (capuchin monkey)

Santana

Brito

et al. 2012

Zygote

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There is a paucity of efficient cryopreservation protocols for primordial follicles enclosed in the ovarian tissue from non-human primates (NHP), in special New World primates. Our objective was to establish an optimal procedure for the recovery of ovarian biopsies from capuchin monkeys. To this end, we adapted a trap door biopsy method. Follicular density and quality of the biopsies were evaluated and ultrasound analysis was performed before and continuously after surgery to assess ovarian structure. Ovarian tissue biopsies recovered by the trap door technique allowed the successful harvesting of primordial follicles from capuchin monkeys, and no complication was recorded. The female cycle was not affected by surgery and no adherence was found thereafter. In conclusion, the adaptation of a trap door biopsy method is a safe procedure and allows recovery of healthy primordial follicles.

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Effects of follicular phase and oocyte–cumulus complexes quality on the protein profile and in vitro oocyte meiosis competence in Cebus apella

Cited by 8 publications

References 24 publications

Short-Term Culture of Ovarian Cortical Strips From Capuchin Monkeys (Sapajus apella): A Morphological, Viability, and Molecular Study of Preantral Follicular Development In Vitro

Short-Term Culture of Ovarian Cortical Strips From Capuchin Monkeys (Sapajus apella): A Morphological, Viability, and Molecular Study of Preantral Follicular Development In Vitro

Assisted Reproductive Techniques on South American Wild Mammals

Adaptation of a trap door technique for the recovery of ovarian cortical biopsies from Cebus apella (capuchin monkey)

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