Effects of Follicle-Stimulating Hormone and Estradiol upon Progesterone Secretion by Porcine Granulosa Cells in Tissue Culture*

Thanki, Kantilal H.; Channing, Cornelia P.

doi:10.1210/endo-103-1-74

Cited by 45 publications

(14 citation statements)

References 12 publications

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“…The cAMP-dependent protein kinase is known to act on several substrates [28], some of whose activities may be modulated by the oocyte-secreted factor. Estrogen is known to decrease granulosa cell progesterone production in many species [22,[25][26][27]. Therefore, maintained or enhanced estrogen production by a complex containing an oocyte may be sufficient to keep progesterone production low, whereas the removal of the oocyte may impair the ability of the cumulus cells to produce estradiol, causing a subsequent elevation in progesterone levels.…”

Section: Discussionmentioning

confidence: 99%

Differential Regulation of Progesterone and Estradiol Production by Mouse Cumulus and Mural Granulosa Cells by a Factor(s) Secreted by the Oocyte

Vanderhyden

Tonary

1995

Biology of Reproduction

127

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Mouse oocytes secrete a factor(s) that inhibits progesterone and enhances estradiol production by cumulus granulosa cells. The purpose of this study was to investigate the mechanisms by which the production of these steroids is modulated. Mouse oocyte-cumulus cell complexes (intact) and complexes from which the oocytes were removed microsurgically (oocytectomized; OOX) were cultured for up to 48 h in the presence of FSH (150 ng/ml) and testosterone (5 x 10(-7) M). For these experiments, all cells were obtained from antral follicles of 24- to 26-day-old mice primed with eCG. Intact complexes produced primarily estradiol, with significant accumulation occurring between 24 and 48 h. In contrast, OOX complexes produced little estradiol but, starting at 18 h of culture, released significantly more progesterone than did intact complexes. Progesterone accumulation in cocultures of denuded oocytes with either OOX complexes or monolayers of mural granulosa cells was significantly reduced compared to that with OOX complexes or mural granulosa cells cultured alone. If dibutyryl cAMP replaced FSH in the cocultures, similar results were obtained, suggesting that the oocyte-secreted steroid-regulating factor acts downstream of the generation of cAMP to inhibit progesterone production. Since estradiol can inhibit progesterone production by granulosa cells, we investigated the possibility that the increased progesterone released by OOX complexes was secondary to the lower estradiol production. Intact complexes cultured in the presence of the nonaromatizable androgen, 5 alpha-dihydrotestosterone, or steroidal (4-hydroxyandrostenedione) or non-steroidal (CGS 16949A) aromatase inhibitors produced little estradiol; however, progesterone production by these complexes was no different from that of estradiol-producing intact complexes. These results suggest that the steroid-regulating factor(s) secreted by occytes acts to regulate granulosa cell production of estradiol and progesterone by independent mechanisms.

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Section: Discussionmentioning

confidence: 99%

Differential Regulation of Progesterone and Estradiol Production by Mouse Cumulus and Mural Granulosa Cells by a Factor(s) Secreted by the Oocyte

Vanderhyden

Tonary

1995

Biology of Reproduction

127

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“…Such a theory requires that there be androgen substrate present, which is the case in the intact follicle, but not in the granulosa cell assay system. However, estrogen can in hibit granulosa cell progesterone secretion both in the presence or absence of FSH [4] so an action of the stimulator solely on aromatase activity cannot explain all of the events occurring during follicular maturation. Fur ther study on this subject is warranted.…”

Section: Discussionmentioning

confidence: 99%

“…Granulosa cells were harvested from small (1 -2 mm) ovarian follicles as previously described [4,13]. The cells were washed twice in serum-free media and inoculated at a density o f 2 X I05 viable cells/well.…”

Section: Granulosa Cell Culture Proceduresmentioning

confidence: 99%

“…The number o f cells in terminated monolayer cul tures was determined by a modified citric acid crystal violet nuclear staining method [4],…”

Section: Granulosa Cell Culture Proceduresmentioning

confidence: 99%

“…There is a sub- stantial increase in LH receptors and a de crease in FSH receptors demonstratable in porcine granulosa cells [1,2]. The changes in gonadotropin receptors are accompanied by an increased ability of LH and a decreased ability of FSH to stimulate progesterone se cretion and synthesis of cyclic 3'-5'-AMP [3,4], The control of these maturation processes appear to involve not only the pituitary hor mones (LH and FSH) along with local intraovarian steroids (androgens, estrogens, and progcstins) but also nonsteroidal factors. Several recently described nonsteroidal ovar ian components include oocyte maturation inhibitor, LH receptor binding inhibitor, FSH receptor binding inhibitor, luleinization inhibitor and inhibin-F [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

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Stimulatory Action of Follicular Fluid Components on Maturation of Granulosa Cells from Small Porcine Follicles

Kolena¹,

Channing²

1985

Horm Res

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Follicular fluid obtained from large (6-12 mm) porcine follicles (LFF) was investigated to determine its stimulatory activity on progesterone secretion and on follicle stimulating hormone (FSH) induction of ¹²⁵I-human chorionic gonadotropin (hCG)-luteinizing hormone (LH) binding sites in porcine granulosa cells in a 4-day culture. Incubation of granulosa cells harvested from small porcine follicles (1-2 mm) with 50% LFF led to stimulation of LH/hCG binding sites and progesterone secretion. After partial purification of pooled LFF or proteins precipitated with 90% ethanol on Sephadex G-100 the greatest stimulatory activity was found in the second protein peak eluted from the column. Chromatography of part of the active fraction on DEAE Sephacel using a continuous gradient of NH4HCO₃ yielded seven protein fractions. The second fraction, which eluted early, contained the majority of the stimulatory activity which was purified about 32-fold compared to native LFF. In contrast, addition of follicular fluid recovered from small porcine follicles inhibited FSH induction of LH/hCG binding sites and progesterone secretion. It can be concluded, that maturation of granulosa cells from small follicles may be enhanced by protein(s) present in LFF, but not in fluid recovered from less mature follicles.

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Growth Factor Modulation of Gonadotropin Action on Porcine Granulosa Cells

Mori

Takakura

Fukuoka

et al. 1990

Advances in Assisted Reproductive Technologies

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Effects of Follicle-Stimulating Hormone and Estradiol upon Progesterone Secretion by Porcine Granulosa Cells in Tissue Culture*

Cited by 45 publications

References 12 publications

Differential Regulation of Progesterone and Estradiol Production by Mouse Cumulus and Mural Granulosa Cells by a Factor(s) Secreted by the Oocyte

Differential Regulation of Progesterone and Estradiol Production by Mouse Cumulus and Mural Granulosa Cells by a Factor(s) Secreted by the Oocyte

Stimulatory Action of Follicular Fluid Components on Maturation of Granulosa Cells from Small Porcine Follicles

Growth Factor Modulation of Gonadotropin Action on Porcine Granulosa Cells

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