Effects of flutropium on experimental models of drug- and allergy-induced rhinitis in guinea pigs.

Abstract

ABSTRACT-The effects of flutropium on histamine (Hist)-induced increase in intra nasal pressure in non-sensitized guinea pigs and nasal mucosa capillary permeability in passively sensitized guinea pigs were investigated. Flutropium (0.3%), atropine (0.3%), diphenhydramine (0.01%) and cimetidine (0.1%) were directly inhaled into the nasal cavities by an ultrasonic nebulizer for 20 min, followed by inhalation of Hist (0.1%) for 10 min. Flutropium, atropine and diphenhydramine had an inhibitory ac tion on the Hist-induced increase in intranasal pressure in guinea pigs. Cimetidine had no effect on this system. In passively sensitized guinea pigs (the challenge was per formed 48 hr after sensitization), a 0.1-1.0 mg/kg injection of flutropium (i.v.) dose dependently inhibited the allergic nasal mucosa capillary permeability. Atropine (10 mg/kg, i.v.) had no inhibitory action on this system. These results suggest that inhala tion into the nasal cavities and i.v. injection of flutropium are effective in ex perimental models of drug and allergy-induced rhinitis of the guinea pig.

A New Model of Experimental Allergic Rhinitis Using Japanese Cedar Pollen in Guinea Pigs

“…The new improved NCL technique in the present experiments has made it possible to obtain more detailed analyses of the pathogenesis of allergic rhinitis because the various released mediators and cell types that migrated into the nasal cavity could be determined. Different from the perfusion method of the nasal cavity through the trachea (3,4), the present NCL has a great advantage be cause it permits a continual investigation of developing or chronic allergic rhinitis induced by repetitive antigen inhalations in the same guinea pig over a long term. Unfavorably, the numbers of neutrophils in the non-sensi tized groups were increased by NCL.…”

“…However, in the majority of these papers, the results were either from the experiments by single challenge by the topical application of intranasal instillation with antigen solution (1,2) or from the method of antigen perfusion of the nasal cavity from the pharynx to the nostrils (3,4). To resolve such drawbacks in experimental allergic rhinitis, we attempted to establish a good experimental model for research on allergic rhinitis using the guinea pig.…”

Involvement of Thromboxane A₂ in Antigen-Induced Nasal Blockage in Guinea Pigs

“…The intranasal pressure was measured by the method of Mizuno et al [17] with some modifications. Briefly, 7 days after the second sen sitization, guinea pigs were anesthetized with sodium pentobarbital (30 mg/kg, i.p.)…”

Inhibitory Effect ofLactobacillus gasseriTMC0356 andLactobacillusGG on Enhanced Vascular Permeability of Nasal Mucosa in Experimental Allergic Rhinitis of Rats

“…Changes in vascular permeability were measured according to the method described by Kojima et al,10) with modifications. 11,12) A cannula was inserted into the trachea on the lung side for maintenance of breathing. The other side of the cannula was connected to an artificial respirator (Shinano SN-480-7, Tokyo) at a frequency 60 times/ min.…”

“…[10][11][12] As for the results, oral administration of Lactobacillus GG and L. gasseri TMC0356 significantly inhibited the increase in nasal vascular permeability in the experimental allergic rhinitis in the rats. To our knowledge, this is the first study to demonstrate the oral activity of lactic acid bacteria in suppressing antigen-stimulated vascular permeability of the nasal mucosa in a model of allergic rhinitis.…”