Effects of Fluoxetine and Potential Antidepressant 8-Trifluoromethyl 1,2,3,4,5-Benzopentathiepin-6-Amine Hydrochloride (TC-2153) on Behavior of Danio rerio Fish in the Novel Tank Test and Brain Content of Biogenic Amines and Their Metabolites

Sinyakova, N. A.; Kulikova, Elizabeth A.; Englevskii, N. A.; Куликов, А. В.

doi:10.1007/s10517-018-4045-6

Cited by 13 publications

(6 citation statements)

References 13 publications

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“…Our finding that exposure to fluoxetine increased boldness matches findings in previous work on sticklebacks (Grécias et al, 2017) and on other fish species (Wong et al, 2013;Ansai et al, 2016;Singer et al, 2016;Sinyakova et al, 2018). Fluoxetine has been shown to also decrease the predator response in sticklebacks (Sebire et al, 2015) and in the fathead minnow (Painter et al, 2009;Weinberger and Klaper, 2014).…”

Section: Discussionsupporting

confidence: 91%

Effects of monoamine manipulations on the personality and gene expression of three-spined sticklebacks

Abbey-Lee

Kreshchenko

Sala

et al. 2019

Journal of Experimental Biology

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Among-individual behavioral differences (i.e. animal personality) are commonly observed across taxa, although the underlying, causal mechanisms of such differences are poorly understood. Animal personality has been correlated with physiological functions as well as fitness-related traits. Variation in many aspects of monoamine systems, such as metabolite levels and gene polymorphisms, has been linked to behavioral variation. Therefore, here we experimentally investigated the potential role of monoamines in explaining individual variation in personality, using two common pharmaceuticals that respectively alter the levels of serotonin and dopamine in the brain: fluoxetine and ropinirole. We exposed threespined sticklebacks, a species that shows animal personality, to either chemical alone or to a combination of the two chemicals, for 18 days. During the experiment, fish were assayed at four time points for the following personality traits: exploration, boldness, aggression and sociability. To quantify brain gene expression on short-and longer-term scales, fish were sampled at two time points. Our results show that monoamine manipulations influence fish behavior. Specifically, fish exposed to either fluoxetine or ropinirole were significantly bolder, and fish exposed to the two chemicals together tended to be bolder than control fish. Our monoamine manipulations did not alter the gene expression of monoamine or stress-associated neurotransmitter genes, but control, untreated fish showed covariation between gene expression and behavior. Specifically, exploration and boldness were predicted by genes in the dopaminergic, serotonergic and stress pathways, and sociability was predicted by genes in the dopaminergic and stress pathways. These results add further support to the links between monoaminergic systems and personality, and show that exposure to monoamines can causally alter animal personality.

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Section: Discussionsupporting

confidence: 91%

Effects of monoamine manipulations on the personality and gene expression of three-spined sticklebacks

Abbey-Lee

Kreshchenko

Sala

et al. 2019

Journal of Experimental Biology

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“…This result agrees with that previously observed earlier in zebrafish [ 33 ]. Earlier we showed that an acute (for 3 h) treatment with fluoxetine did not affect the 5-HT level in the brain of zebrafish [ 38 ]. Now we found that prolonged treatment with fluoxetine decreased 5-HT and 5-HIAA levels in the brain of zebrafish.…”

Section: Discussionmentioning

confidence: 99%

“…Acute [ 35 , 37 , 38 ] or chronic [ 34 , 35 ] treatment with fluoxetine increases time spent near the tank’s surface (“surface dwelling”) in zebrafish. Recently we showed that acute treatment with imipramine also induced “surface dwelling” in zebrafish [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Tryptophan Hydroxylase 2 Deficiency Modifies the Effects of Fluoxetine and Pargyline on the Behavior, 5-HT- and BDNF-Systems in the Brain of Zebrafish (Danio rerio)

Evsiukova

Базовкина

Bazhenova

et al. 2021

IJMS

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The mechanisms of resistance to antidepressant drugs is a key and still unresolved problem of psychopharmacology. Serotonin (5-HT) and brain-derived neurotrophic factor (BDNF) play a key role in the therapeutic effect of many antidepressants. Tryptophan hydroxylase 2 (TPH2) is the rate-limiting enzyme in 5-HT synthesis in the brain. We used zebrafish (Danio rerio) as a promising model organism in order to elucidate the effect of TPH2 deficiency caused by p-chlorophenylalanine (pCPA) on the alterations in behavior and expression of 5-HT-related (Tph2, Slc6a4b, Mao, Htr1aa, Htr2aa) and BDNF-related (Creb, Bdnf, Ntrk2a, Ngfra) genes in the brain after prolonged treatment with two antidepressants, inhibitors of 5-HT reuptake (fluoxetine) and oxidation (pargyline). In one experiment, zebrafish were treated for 72 h with 0.2 mg/L fluoxetine, 2 mg/L pCPA, or the drugs combination. In another experiment, zebrafish were treated for 72 h with 0.5 mg/L pargyline, 2 mg/L pCPA, or the drugs combination. Behavior was studied in the novel tank diving test, mRNA levels were assayed by qPCR, 5-HT and its metabolite concentrations were measured by HPLC. The effects of interaction between pCPA and the drugs on zebrafish behavior were observed: pCPA attenuated “surface dwelling” induced by the drugs. Fluoxetine decreased mRNA levels of Tph2 and Htr2aa genes, while pargyline decreased mRNA levels of Slc6a4b and Htr1aa genes. Pargyline reduced Creb, Bdnf and Ntrk2a genes mRNA concentration only in the zebrafish treated with pCPA. The results show that the disruption of the TPH2 function can cause a refractory to antidepressant treatment.

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“…The NTT (20 × 20 × 5 cm, made of plexiglass; Open Science, Krasnogorsk, Russia) was divided by an imaginary marker line into top and bottom zones of equal area [ 23 , 29 ]. Trials were recorded with a CNE-CWC1 web camera (Canyon, ASBIS, Limassol, Cyprus) for further analyses, and then they were processed offline using EthoVision XT 11.5 software (Noldus IT, Wageningen, Netherlands) to calculate the total distance traveled (cm), mean and maximum velocity (cm/s), number of freezes (velocity < 2 cm/s), total freezing duration (s), total time spent in the top zone (s), number of entries into the top zone, and latency of the first bottom–top transition (s) [ 23 , 29 , 30 , 31 , 32 ].…”

Section: Methodsmentioning

confidence: 99%

Novel Chromone-Containing Allylmorpholines Induce Anxiolytic-like and Sedative Effects in Adult Zebrafish

et al. 2022

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Chromone-containing allylmorpholines (CCAMs) are a novel class of compounds that have demonstrated acetyl- and butyryl-cholinesterase-inhibiting and N-methyl-D-aspartate (NMDA) receptor-blocking properties in vitro, but their in vivo pharmacological activity remains underexplored. In this work, we evaluated the psychotropic activity of five different CCAMs (1 (9a), 2 (9j), 3 (9l), 4 (33a), and 5 (33b)) using the novel tank test (NTT) and light/dark box (LDB) test in adult zebrafish. The CCAMs were screened in the NTT at a range of concentrations, and they were found to induce a dose-dependent sedative effect. Compound 4 (33a) was also evaluated using the LDB test, and it was found to have anxiolytic-like properties at low concentrations. To assess the potential contribution of the glutamate and cholinergic mechanisms in the effects of the CCAMs, we conducted experiments with pre-exposure to putative antagonists, NMDA and biperiden. Neither biperiden nor NMDA were able to diminish or cancel the effects of the CCAMs, countering the in vitro data obtained in previous studies. The apparent discrepancy could be related to the specifics of CCAM metabolism or to the interspecies differences between the putative target proteins, possibly due to the relatively low identity percentage of their sequences. Although further research in mammals is required in order to establish their pharmacological properties, novel CCAMs may represent an appealing group of psychoactive drug candidates.

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Effects of Fluoxetine and Potential Antidepressant 8-Trifluoromethyl 1,2,3,4,5-Benzopentathiepin-6-Amine Hydrochloride (TC-2153) on Behavior of Danio rerio Fish in the Novel Tank Test and Brain Content of Biogenic Amines and Their Metabolites

Cited by 13 publications

References 13 publications

Effects of monoamine manipulations on the personality and gene expression of three-spined sticklebacks

Effects of monoamine manipulations on the personality and gene expression of three-spined sticklebacks

Tryptophan Hydroxylase 2 Deficiency Modifies the Effects of Fluoxetine and Pargyline on the Behavior, 5-HT- and BDNF-Systems in the Brain of Zebrafish (Danio rerio)

Novel Chromone-Containing Allylmorpholines Induce Anxiolytic-like and Sedative Effects in Adult Zebrafish

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