Effects of fluoride on liver apoptosis and Bcl-2, Bax protein expression in freshwater teleost, Cyprinus carpio

Cao, Jianhua; Chen, Jianjie; Wang, Jundong; Jia, Ru; Xue, Wujun; Luo, Yu; Xi, Gan

doi:10.1016/j.chemosphere.2013.01.037

Cited by 100 publications

(28 citation statements)

References 21 publications

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“…Some reports have indicated that fluoride could cause lipid oxidative damage of cell membranes, DNA damage, and promote apoptosis [6], and have found that fluoride could result in the apoptosis of rat spermatogenic cells at a certain dose and time [30]. In this study, fluoride stimulated apoptosis in liver tissues.…”

Section: Discussionmentioning

confidence: 81%

“…The cDNA was reversed transcription (RT) from 1 μg of total RNA with primer oligo(dT)18 by using superscript II RNase (Reverse Transcriptase; Invitrogen Inc., USA). The caspase-3 and −9 cDNA were amplified by polymerase chain reaction (PCR) based on a previously published rat cDNA primer sequence (6)(7)(8). β-Actin was the internal standard.…”

Section: Immunohistochemistry Studiesmentioning

confidence: 99%

“…Many reports have shown that F can cause progressive degeneration of the structure and functions of skeletal muscles, the brain, and the spine. In addition, it increases the aerobic metabolism and promotes alterations in the metabolism of cellular free radicals in several organs such as the liver, kidneys, and heart [6][7][8][9][10]. Free radicals cause toxicity by attacking membrane phospholipids, leading to membrane injury through lipid peroxidation, depolarization of the mitochondrial membrane, and apoptosis [11].…”

Section: Introductionmentioning

confidence: 98%

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Effects of Fluoride on DNA Damage and Caspase-Mediated Apoptosis in the Liver of Rats

Song

Huang

Gao

et al. 2015

Biol Trace Elem Res

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Fluoride compounds are abundant and widely distributed in the environment at a variety of concentrations. Further, fluoride induces toxic effects in target organs such as the liver. In this study, we investigated liver histopathology, DNA damage, apoptosis, and the mRNA and protein expressions of caspase-3 and -9 in the rat livers by administering varying concentrations of fluoride (0, 50, 100, 200 mg/L ) for 120 days. The results showed fluoride-induced morphological changes and significantly increased apoptosis and DNA damage in rats exposed to fluoride, especially in response to higher doses. The immunohistochemical and qRT-PCR results indicated that caspase-3, caspase-9 protein positive expression and mRNA relative expression enhanced with increasing NaF concentration. In summary, our findings suggest that chronic exposure to fluoride causes damages to liver histopathology and leads to liver apoptosis through caspase-mediated pathways.

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Section: Discussionmentioning

confidence: 81%

Section: Immunohistochemistry Studiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Effects of Fluoride on DNA Damage and Caspase-Mediated Apoptosis in the Liver of Rats

Song

Huang

Gao

et al. 2015

Biol Trace Elem Res

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“…Bcl-2 and its family were reported to be distributed in different tissues, including liver, breast, kidney, and brain tissue [Lin et al, 1999;Bannova et al, 2005;Yu et al, 2010;Cao et al, 2013;Wang et al, 2015]. However, it is currently unclear whether Bcl-2 is expressed in the auditory cortex.…”

Section: Discussionmentioning

confidence: 99%

Differential Expression of Bcl-2 in the Cochlea and Auditory Cortex of a Mouse Model of Age-Related Hearing Loss

Huang

Xiong²,

Yang³

et al. 2016

Audiol Neurotol

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Bcl-2, the first gene shown to be involved in apoptosis, is a potent regulator of cell survival and known to have protective effects against a variety of age-related diseases. However, the possible relationship between hearing and Bcl-2 expression in the cochlea or auditory cortex of C57BL/6 mice, a mouse model of age-related hearing loss, is still unknown. Using RT-PCR, immunohistochemistry, and Western blot analysis, our results show that Bcl-2 is strongly expressed in the inner hair cells and spiral ganglion neurons of young mice. In addition, moderate Bcl-2 expression is also detected in the outer hair cells and in the neurons of the auditory cortex. A significant reduction of Bcl-2 expression in the cochlea or auditory cortex is also associated with elevated hearing thresholds and hair cell loss during aging. The expression pattern of Bcl-2 in the peripheral and central auditory systems suggests that Bcl-2 may play an important role in auditory function serving as a protective molecule against age-related hearing loss.

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“…However, exposure to excessive F may be severely harmful to human health [1]. In addition to well-known effects on the skeleton and teeth [2][3][4][5][6], chronic F poisoning causes pathological changes in non-skeletal tissues, as exhibited by various symptoms and pathological changes in humans and animals [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Pro-inflammatory Cytokines Are Involved in Fluoride-Induced Cytotoxic Potential in HeLa Cells

Wang

Zhou

Cao

et al. 2016

Biol Trace Elem Res

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This study was designed to investigate the pro-inflammatory cytokines and their involvement in the cytotoxic potential of fluoride (F) in HeLa cells. HeLa cells were cultured with varying F concentrations (1-50 mg/L) for 48 h, and treatment effects were analyzed. The viability of HeLa cells was determined with a colorimetric method. The concentrations of IL-1β, IL-2, IL-6, and TNF-a in culture supernatant were measured through enzyme linked immunosorbent assay (ELISA). The mRNA expression levels of IL-1β, IL-2, IL-6 and TNF-a were subjected to transcript analysis and quantified through reverse transcription real-time PCR. Results showed that 10, 20 and 50 mg/L F significantly decreased the viability of HeLa cells incubated for 24 and 48 h. With their cytotoxic effect, the concentrations of IL-1β, IL-2, IL-6, and TNF-a decreased significantly in response to F, especially at 20 and 50 mg/L for 48 h. The mRNA expression levels of IL-1β, IL-2, IL-6, and TNF-a were downregulated at 50 mg/L F for 48 h. Therefore, F inhibited HeLa cell growth; as such, F could be used to alleviate the inhibition of pro-inflammatory cytokine expression.

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Effects of fluoride on liver apoptosis and Bcl-2, Bax protein expression in freshwater teleost, Cyprinus carpio

Cited by 100 publications

References 21 publications

Effects of Fluoride on DNA Damage and Caspase-Mediated Apoptosis in the Liver of Rats

Effects of Fluoride on DNA Damage and Caspase-Mediated Apoptosis in the Liver of Rats

Differential Expression of Bcl-2 in the Cochlea and Auditory Cortex of a Mouse Model of Age-Related Hearing Loss

Pro-inflammatory Cytokines Are Involved in Fluoride-Induced Cytotoxic Potential in HeLa Cells

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