Effects of Fluoride, Lithium, and Strontium on Growth and Acid Production of Mutans Streptococci and Actinomyces viscosus

Eisenberg, A.D.; Oldershaw, M.D.; Curzon, M.E.J.; Handelman, Stanley L.

doi:10.1159/000261364

Cited by 9 publications

(3 citation statements)

References 15 publications

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“…2 (1,560 ppm F), but this value steadily increased to a maximum of 7.2 ppm with sample No. 7 (33,600 ppm F), a concentration known to inhibit bacterial and plaque glycolysis [van Loveren, 1990;Eiscnberg et al, 1991], Moreover, with sample No. 2 most mineral had to dissolve before the 0.49 ppm lev el was attained whereas with sample No.…”

Section: Discussionmentioning

confidence: 99%

Stoichiometry of Fluoride Release from Fluorhydroxyapatite during Acid Dissolution

Pearce

Guha-Chowdhury

Iwami³

et al. 1995

Caries Res

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Release of F from fluorhydroxyapatite (FHAp) during acid dissolution was studied to validate the use of this mineral as a plaque reservoir of F. FHAp minerals having a wide range of F concentrations were synthesised by aqueous precipitation, and samples repeatedly exposed to 50 mM lactic acid solution, pH 4.5, or similar lactic/acetic/formic acid mixtures, until dissolution was complete. While the Ca/P ratio in solution remained relatively constant and close to the ratio in the solid, the solution F/Ca ratio invariably changed during dissolution. During initial stages the F/Ca solution ratio was lower than in the solid but rose to reach a plateau higher than in the solid as dissolution progressed, an effect that was more pronounced with low-F FHAp. With these minerals the plateau F/Ca level never reached 0.2, suggesting that a F-enriched FHAp rather than pure fluorapatite precipitates during dissolution. It is concluded that a high-F FHAp mineral would best serve as an apatitic plaque reservoir of F

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Section: Discussionmentioning

confidence: 99%

Stoichiometry of Fluoride Release from Fluorhydroxyapatite during Acid Dissolution

Pearce

Guha-Chowdhury

Iwami³

et al. 1995

Caries Res

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“…Furthermore, there is much evidence showing that fluoride can affect virulence factors of cariogenic bacteria [Marsh and Bradshaw, 1990]. For instance, fluoride can inhibit the acid production and acid tolerance of cariogenic bacteria such as S. mutans in planktonic state [Eisenberg et al, 1991]. Furthermore, fluoride can also affect acid production, acid tolerance and GTF secretion of S. mutans in biofilms [Hayacibara et al, 2003;Pandit et al, 2011], indicating that it could reduce dental caries by decreasing the virulence of cariogenic biofilms.…”

mentioning

confidence: 99%

Relationship between Fluoride Concentration and Activity against Virulence Factors and Viability of a Cariogenic Biofilm: in vitro Study

et al. 2013

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Despite widespread use of various concentrations of fluoride for the prevention of dental caries, the relationship between fluoride concentration and activity against cariogenic biofilms has not been much studied. Herein we investigated the relationship between fluoride concentration and activity against virulence factors and viability of Streptococcus mutans biofilms. S. mutans biofilms were formed on saliva-coated hydroxyapatite discs. The 70-hour-old biofilms were exposed to 0, 1, 3, 10, 30, 100, 300, 1,000 or 2,000 ppm F^-. The changes of virulence factors and viability of the biofilms were analyzed using biochemical methods and laser scanning confocal fluorescence microscopy. At 1-2,000 ppm F^-, the activity of fluoride against acid production, acid tolerance, and extracellular polysaccharide formation of S. mutans biofilms accurately followed a sigmoidal pattern of concentration dependence (R² = 0.94-0.99), with EC₅₀ values ranging from 3.07 to 24.7 ppm F^-. Generally, the activity of fluoride against the virulence factors was concentration-dependently augmented in 10-100 ppm F^- and did not increase further at concentrations higher than 100 ppm F^-. However, fluoride did not alter glucosyltransferase activity and viability of S. mutans biofilm cells in all concentrations tested. These results can provide a basis for the selection of appropriate fluoride concentrations that reduce the physiological ability of cariogenic biofilms.

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“…However, the incorporation of fl uoride (as NaF) into microbiological culture medium results in the selective isolation of oral Actinomyces spp., including A. naeslundii [Beighton and Colman, 1976;Zylber and Jordan, 1982] from oral samples. Further, acid production by Actinomyces viscosus [ A. naeslundii ] was reported to be less susceptible to inhibition by fl uoride than acid production by mutans streptococci [Eisenberg et al, 1991]. In a simple batch model system [Beighton and McDougal, 1977], NaF inhibited Streptococcus mutans but not A. viscosus [ A. naeslundii ] and in a chemostat model, the addition of 10 ppm NaF to a mixed oral bacterial community, not including Lactobacillus sp.…”

Section: Discussionmentioning

confidence: 99%

Effect of Withdrawal of Fluoride-Containing Toothpaste on the Interproximal Plaque Microflora

Brailsford

Gilbert²,

Clark³

et al. 2005

Caries Res

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To compare the effects of fluoride-containing and fluoride-free toothpaste on plaque microflora, 15 subjects were enrolled in a double-blind crossover trial. All subjects used a fluoride toothpaste for 7 days before the trial started. Then, 4 interproximal sites per subject were professionally cleaned and subjects used one of the toothpastes for 5 days. On the 5th day plaque was collected from 2 sites, 12 and 6 h after toothpaste use. There was no difference between the groups in the numbers or proportions of aciduric bacteria (recovered at pH 4.8 or 5.2), or of yeasts, neisseriae, lactobacilli or streptococci (total or individual species, including Streptococcus mutans). However, the numbers and proportions of Gram-positive pleomorphic rods, primarily Actinomyces naeslundii, increased in 6-hour samples from subjects using fluoride toothpaste. The data suggest that the anti-caries effect of fluoride toothpaste is not mediated primarily through effects on the plaque microflora, although effects on plaque physiology could be important.

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Effects of Fluoride, Lithium, and Strontium on Growth and Acid Production of Mutans Streptococci and Actinomyces viscosus

Cited by 9 publications

References 15 publications

Stoichiometry of Fluoride Release from Fluorhydroxyapatite during Acid Dissolution

Stoichiometry of Fluoride Release from Fluorhydroxyapatite during Acid Dissolution

Relationship between Fluoride Concentration and Activity against Virulence Factors and Viability of a Cariogenic Biofilm: in vitro Study

Effect of Withdrawal of Fluoride-Containing Toothpaste on the Interproximal Plaque Microflora

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