Effects of FK506 (tacrolimus hydrate) on chronic oxazolone-induced dermatitis in rats

Fujii, Yasutomo; Takeuchi, Hiroko; Tanaka, Kunihito; Sakuma, Shozo; Ohkubo, Y.; Mutoh, Seitaro

doi:10.1016/s0014-2999(02)02554-2

Cited by 36 publications

(45 citation statements)

References 34 publications

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“…8) Fujii et al developed an oxazolone-induced animal model of experimental psoriasis. 9) This psoriatic animal model is a chronic inflammation model and significantly induces the expression levels of COX-2 and inducible NO synthase (iNOS) in monocytes and macrophages and IFN-g and interleukin (IL)-4 in Th cells. The ginsenosides have been reported to show various biological activities including antiinflammatory activity, antiallergic, endothelium-independent aorta relaxation, and antitumor effects.…”

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Inhibitory Effects of Korean Red Ginseng and Its Genuine Constituents Ginsenosides Rg3, Rf, and Rh2 in Mouse Passive Cutaneous Anaphylaxis Reaction and Contact Dermatitis Models

Bae

Han

Shin

et al. 2006

Biological & Pharmaceutical Bulletin

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Korean red ginseng (KRG, the steamed root of Panax ginseng C.A. MEYER, family Araliaceae) is frequently used as a crude substance taken orally in Asian countries as a traditional medicine. The major components of raw ginseng are ginsenosides, which contain an aglycone with a dammarane skeleton.1,2) Many types of saponins, such as ginsenosides Rb1, Rb2, Rc, and Rf, have been isolated. The ginsenosides Rg3, Rf, and Rh2 are genuine saponins in KRG and heatprocessed ginseng.3,4) Ginsenosides Rg3 and Rh2 were produced from protopanaxadiol ginsenosides by steaming to prepare KRG. 5)Psoriasis is a chronic inflammatory skin disorder. Psoriasis patients have been shown to have interferon (IFN)-g producing Th1 bias in lesion skin and peripheral blood and are thought to develop cytokine networks of Th1 cells, resulting in keratinocyte hyperplasia and angiogenesis.6,7) Cylcooxygenase (COX)-2 is also induced in angiogenesis-related diseases such as rheumatoid arthritis and psoriasis. 8) Fujii et al. developed an oxazolone-induced animal model of experimental psoriasis.9) This psoriatic animal model is a chronic inflammation model and significantly induces the expression levels of COX-2 and inducible NO synthase (iNOS) in monocytes and macrophages and IFN-g and interleukin (IL)-4 in Th cells. The ginsenosides have been reported to show various biological activities including antiinflammatory activity, antiallergic, endothelium-independent aorta relaxation, and antitumor effects.10-13) Tachikawa et al. reported that ginsenoside Rg3 suppressed histamine release from mast cells due to stimulation with compoumd 48/80 in vitro, 14) and Ro et al. reported that ginsenosides Rb1 and Rc also partly inhibited the release of histamine and leukotrienes during the activation of guinea pig lung mast cells in vitro. 15) We also reported the antiallergic and antiinflammatory effects of ginsenoside Rh1, antiallergic and passive cutaneous anaphylaxis (PCA) reaction-inhibitory effects of compound K, and antiallergic effects of ginsenoside Rh2. [16][17][18] However, the antiallergic and antipsoriatic effects of KRG and genuine ginsenoside Rf have not been thoroughly studied. We therefore investigated the PCA reaction-inhibitory and antiallergic and antipsoriatic effects of the KRG saponin fraction (KRGS) and its constituents ginsenosides Rg3, Rf, and Rh2. MATERIALS AND METHODS Materials p-Nitrophenyl-N-acetyl-b -D-glucosaminide,Freund's complete adjuvant, anti-dinitrophenol (DNP)-IgE, DNP-human serum albumin (HSA), lipopolysaccharide (LPS), Evans blue, disodium cromoglycate (DSCG), trichloroacetic acid, betamethasone, and azelastine were purchased from Sigma Chemical Co. (St. Louis, MO, U.S.A.). KRG water extract, ginsenoside Rf, and ginsenoside Rh2 were donated by KT&G (Seoul, Korea). KRGS and ginsenoside Rg3 were prepared according to our previous method. 6)KRGS, which is the BuOH fraction of KRG, contains ginsenosides Rb1 (16.3%), Rf (7.4%), Rg3 (4.6%), and Rh2 (0.1%) as the main constituents.Assay of Antiallergic Activity in the RBL 2H3 Cell...

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Inhibitory Effects of Korean Red Ginseng and Its Genuine Constituents Ginsenosides Rg3, Rf, and Rh2 in Mouse Passive Cutaneous Anaphylaxis Reaction and Contact Dermatitis Models

Bae

Han

Shin

et al. 2006

Biological & Pharmaceutical Bulletin

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“…9) This dermatitis was accompanied by sustained swelling, predominant epidermal hyperplasia and marked infiltration of inflammatory cells consisting of monocytes, granulocytes and macrophages, but not eosionphils. In the present study, the oxazolone-induced dermatitis was also accompanied by substained swelling and predominant epidermal hyperplasia as reported by Fujii et al 9) IFN-g and TNF-a, which are cytokines involved in chronic skin inflammatory disease, [14][15][16] and COX-2, which is an acute marker of acute inflammatory disease were induced. CHD, which is a potent inhibitor against NO and PGE2 productions in LPS-induced RAW264.7 cells, 12) significantly inhibited sustained swelling (thickness) of mice ear induced by oxazolone as well as mRNA levels of COX-2.…”

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confidence: 99%

“…[5][6][7][8][9] Corticosteroids are well known to have potent anti-inflammatory effects, but topical use can cause intense skin atrophy, one of the serious side effects limiting their uses for chronic skin diseases. 5,7) Repeated application of corticosteroids on dorsal skin of rats also causes dramatic skin atrophy.…”

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confidence: 99%

“…Contact Hypersensitivity An oxazolone-induced dermatitis was measured according to the previous method of Fujii et al 9) BALB/c mice were sensitized by application of 100 ml of 1.5% oxazolone in ethanol to the abdomen. Then a total of 20 ml of 1% oxazolone in a mixture of acetone and olive oil (4 : 1) was applied to both sides of the mouse ear every 3 d starting from 7 d after sensitization.…”

Section: )mentioning

confidence: 99%

“…[1][2][3][4] Cyclooxygenase (COX)-2 inhibitors non-steroidal anti-inflammatory drugs and corticosteroids and immunosuppressants FK-506 and cyclosporine A for Th1 cells have been used clinically for psoriasis. [5][6][7][8][9] Corticosteroids are well known to have potent anti-inflammatory effects, but topical use can cause intense skin atrophy, one of the serious side effects limiting their uses for chronic skin diseases.…”

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Effect of Chunghyuldan in Chronic Oxazolone-Induced Mouse Dermatitis

Wee

Shin

Bae

et al. 2005

Biological & Pharmaceutical Bulletin

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Psoriasis is a chronic and inflammatory skin disorder. Psoriasis patients have been shown to have the bias of interferon (IFN)-g producing Th1 and cyclooxygenase (COX)-induced macrophage in lesion skin and peripheral blood. [1][2][3][4] Cyclooxygenase (COX)-2 inhibitors non-steroidal anti-inflammatory drugs and corticosteroids and immunosuppressants FK-506 and cyclosporine A for Th1 cells have been used clinically for psoriasis. [5][6][7][8][9] Corticosteroids are well known to have potent anti-inflammatory effects, but topical use can cause intense skin atrophy, one of the serious side effects limiting their uses for chronic skin diseases.5,7) Repeated application of corticosteroids on dorsal skin of rats also causes dramatic skin atrophy. FK-506 and cyclosporine A also exhibited side effects, such as severe nephrotoxicity and neurotoxicity. 6) Therefore, herbal medicines for clinical uses, such as Centella asiatica extract, Orengedokto and Sofusan, should be developed. 10,11) Nevertheless, antipsoriatic effects of these herbal medicines have not been thoroughly studied.During the screening program to discover such agents from herbal medicines, Chunghyuldan (CHD, Daio-Orengedokuto in Japanese) exhibited the anti-inflammatory effect. 12)Therefore, we evaluated the antipsoriatic effect of CHD in the oxazolone-induced mouse contact dermatitis model by topical administration and measured mRNA levels of COX-2 and some cytokines. CHD and metabolized CHD were prepared according to the previously reported methods of Cho et al. MATERIALS AND METHODS Materials 12)Animals The female BALB/c mice (20-25 g) were supplied from Orient Experimental Animal Breeding Center (Seoul, Korea). All animals were housed in wire cages at 20-22°C and 50Ϯ10% humidity, fed standard laboratory chow (Orient Experimental Animal Breeding Center, Seoul, Korea) and allowed water ad libitum. All procedures relating to animals and their care conformed to the international guidelines 'Principles of Laboratory Animals Care' (NIH publication no. 85-23, revised 1985).Contact Hypersensitivity An oxazolone-induced dermatitis was measured according to the previous method of Fujii et al.9) BALB/c mice were sensitized by application of 100 ml of 1.5% oxazolone in ethanol to the abdomen. Then a total of 20 ml of 1% oxazolone in a mixture of acetone and olive oil (4 : 1) was applied to both sides of the mouse ear every 3 d starting from 7 d after sensitization. Ear thickness was measured using a Digimatic Micrometer (Mitsutoyo Co., Tokyo, Japan) 72 h after each application of the oxazolone, test agents were applied in a total volume of 20 ml to both sides of the ear 30 min before and 3 h after each application of oxazolone RT-PCR Analysis Ear tissue extract for RT-PCR analysis were performed by the modified method of Chi et al. 13)Briefly, ears were excised 6 h after the last application of oxazolone, freezed in liquid nitrogen and homogenized by a mortar and pestle prechilled in liquid nitrogen. Total RNA was extracted by using TRI reagent according to the ma...

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What Must a Model Display for Proof as a Model of Contact Dermatitis?

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Animal Models of T Cell-Mediated Skin Diseases

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Effects of FK506 (tacrolimus hydrate) on chronic oxazolone-induced dermatitis in rats

Cited by 36 publications

References 34 publications

Inhibitory Effects of Korean Red Ginseng and Its Genuine Constituents Ginsenosides Rg3, Rf, and Rh2 in Mouse Passive Cutaneous Anaphylaxis Reaction and Contact Dermatitis Models

Inhibitory Effects of Korean Red Ginseng and Its Genuine Constituents Ginsenosides Rg3, Rf, and Rh2 in Mouse Passive Cutaneous Anaphylaxis Reaction and Contact Dermatitis Models

Effect of Chunghyuldan in Chronic Oxazolone-Induced Mouse Dermatitis

What Must a Model Display for Proof as a Model of Contact Dermatitis?

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