Effects of fish meal under different storage conditions on growth, serum biochemical indices and antioxidant capacity for juvenile grouper Epinephelus coioides

Abstract

This research aimed to assess effects of fish meal under different storage conditions on growth and antioxidant capacity for juvenile grouper within eight weeks. The healthy fish samples (initial body weight = 18.75 ± 0.05 g) were irregularly distributed to 0.5 m3 barrels with 28 fish per barrel, fed with each diet in the three replicate groups. Fish meals (FMs) were stored at 4°C (experiment 1) and room temperature (30 ± 2°C, experiment 2) for 45, 90 and 135 days, and the data of VBN, HA, AV and TBA were detected. Seven iso‐nitrogenous and iso‐lipidic diets were prepared and denoted as fresh fish meal FFM (control group), 4‐FM45, 4‐FM90, 4‐FM135, RT‐FM45, RT‐FM90 and RT‐FM135, respectively. Prolonged storage time, the volatile base nitrogen, acid value, histamine and thiobarbituric acid contents of FM increased significantly (p < .05). The growth condition and feed utilization of experimental fish were not significantly affected (p > .05). Prolonged storage time, in experiment 1, the catalase (CAT), the superoxide dismutase (SOD) activities and the malondialdehyde (MDA) contents initially increased dramatically and then decreased (p < .05). The reactivity of CAT mRNA in FFM group was significantly higher than that in other treatment groups (p < .05). In experiment 2, CAT activity decreased significantly (p < .05), whereas the MDA contents increased sharply (p < .05). SOD activity and CAT expression in the liver initially increased and then decreased (p < .05). The mRNA level and enzyme activity of trypsin decreased (p < .05) in experiment 1 and 2. In summary, storage of FM (within 135 days) cannot improve the growth and feed utilization of juvenile grouper but weaken the antioxidant capacity of the liver.

Changes of Volatile Flavor Compounds in Large Yellow Croaker (Larimichthys crocea) during Storage, as Evaluated by Headspace Gas Chromatography–Ion Mobility Spectrometry and Principal Component Analysis

“…More specifically, after 120 d, the AV, POV, p-AV, and CDV were 7.62 ± 0.14 mg/g, 23.67 ± 0.55 meq/kg, 2.33 ± 0.03, and 33.57 ± 0.89, respectively. This observed increase in the AV is also consistent with previous studies [ 24 , 25 ] and is closely related to the free fatty acid content [ 26 , 27 ]. Previously, it was reported that the oxidation of fish meat during storage causes the breakage of ester bonds, which releases a large amount of free fatty acids and increases the AV [ 28 ].…”

Effects of Dietary Glycerol Monolaurate on Growth, Antioxidant Capacity and Lipid Metabolism in Cage-Farmed Pompano (Trachinotus ovatus) Juveniles

“…Free radicals scavenging is a function of the antioxidant system in the body (Liang et al, 2020a). T-AOC is the total level of various antioxidant macromolecules, antioxidant small molecules and enzymes within a system (Lin et al, 2021a). SOD GSH-Px are antioxidant enzyme systems that are closely related to free radical scavenging functions and protect important cellular macromolecules and organelles from oxidative damage (Hu et al, 2019).…”

“…All raw material was crushed through a 60-mesh sieve, mixed through a V-type vertical mixer (JS-14S; Zhejiang Zhengtai Electric Co., Ltd.), followed by adding with oil and water, and then pelleted (2.5 mm diameter) making use of a double screw extruder (F-75; South China University of Technology) (Song et al, 2010;Liang et al, 2020a). After the prepared experimental feed was naturally dried to about 10% moisture, it was sealed in a vacuum-packed bag and stored at -20°C until it was fed (He et al, 2021;Lin et al, 2021a).…”

A Challenge to Conventional Fish Meal: Effects of Soy Protein Peptides on Growth, Histomorphology, Lipid Metabolism and Intestinal Health for Juvenile Pompano Trachinotus ovatus

“…All raw material was crushed through a 60-mesh sieve, mixed thorough a V-type vertical mixer (JS-14S; Zhejiang Zhengtai Electric Co., Ltd.), followed by adding with oil and water, and then pelleted (2.5 mm diameter) making use of a double screw extruder (F-75; South China University of Technology). After the prepared experimental feed was naturally dried to about 10% moisture, it was sealed in a vacuum-packed bag and stored at −20 • C until it was fed (He et al, 2021;Lin et al, 2021).…”