Effects of Film Mulching on Soil Microbial Diversity and Community Structure in the Maize Root Zone under Drip Irrigation in Northwest China

Abstract

Mulching is a widely used agricultural water conservation measure in the semiarid regions of Northwest China. In order to explore the response process of different film mulching methods to soil microorganisms, we characterized the effect of different film mulching methods on soil microbial diversity and community structure characteristics in the root zone of drip-irrigated maize during the heading and maturity stages using high-throughput sequencing of 16SrDNA and ITS amplicons combined with bioinformatics analysis. Full mulching (FM) was contrasted to controls of no mulching (NM) and half-mulching (HM), yielding an order of microbial diversity, abundance, and evenness scores of HM > FM > NM. The HM and FM treatments reduced the relative abundance of Proteobacteria and Actinobacteria (the most abundant bacteria) in the bacterial community structure but increased that of Acidobacteria and Chloroflexi. In the fungal community structure, HM decreased the abundance of Sordariomycetes but increased that of Eurotiomycetes (the most abundant fungi). The abundance and community structure of bacteria were significantly correlated with soil temperature and those of fungi with pH. HM improved network complexity and competitive relationships among bacteria, while FM increased the relationship between fungal groups and the symbiosis of fungal communities. HM significantly increased maize yield (20.37% and 6.01% above NM and FM, respectively). In summary, full mulching was more favorable than no mulching for soil microbial diversity and community structure composition, but soil microbial diversity and yield responded better to half-mulching. These results provide a background for improving the yield of drip-irrigated maize and protecting the microbial ecosystems of farmland soils.