Effects of ferroxidase activity and species on ceruloplasmin mediated iron uptake by BT325 cells

Xie, Jun; Tsoi, Yin King; Chang, Yan; Ke, Ya; Qian, Zhong Ming

doi:10.1016/s0169-328x(01)00336-9

Cited by 14 publications

(13 citation statements)

References 27 publications

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“…Early studies established that CP functions to promote iron release from storage cells to plasma in vivo [7]. However, some in vitro studies have reported that CP might play a role in iron uptake in addition to iron release [8–11]. Fpn1 is the first and only member of the SLC40 family of transporters that has been shown to mediate the efflux of iron from cells.…”

Section: Introductionmentioning

confidence: 99%

Role of hepcidin in murine brain iron metabolism

Wang

Duan

et al. 2009

Cell. Mol. Life Sci.

123

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Brain iron homeostasis is maintained by a balance of both iron uptake and release, and accumulating evidence has revealed that brain iron concentrations increase with aging. Hepcidin, an iron regulatory hormone produced by hepatocytes in response to inflammatory stimuli, iron, and hypoxia, has been shown to be the long-sought hormone responsible for the regulation of body iron balance and recycling in mammals. In this study, we report that hepcidin is widely expressed in the murine brain. In cerebral cortex, hippocampus and striatum, hepcidin mRNA levels increased with aging. Injection of hepcidin into the lateral cerebral ventricle resulted in decreased Fpn1 protein levels in cerebral cortex, hippocampus, and striatum. Additionally, treatment of primary cultured neurons with hepcidin caused decreased neuronal iron release and Fpn1 protein levels. Together, our data provide further evidence that hepcidin may be involved in the regulation of brain iron metabolism.

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Section: Introductionmentioning

confidence: 99%

Role of hepcidin in murine brain iron metabolism

Wang

Duan

et al. 2009

Cell. Mol. Life Sci.

123

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“…However, it has also been suggested that CP might play a role not only in iron efflux from brain neurons, but also in iron influx into brain neurons via its ferroxidase activity. The accumulated data, including the location of CP in the brain, in vitro studies on the effect of CP on iron transport (Mukhopadhyay et al, 1998; Qian et al, 2001a; Xie et al, 2002), the similarities between Fet3p (a multicopper oxidase in Saccharomyces cerevisiae ) (Dancis et al, 1994) and CP and the existence of spontaneous oxidation activity (Fe 2+ + H 2 O 2 → Fe 3+ + highly reactive free radicals) in the brain (Young et al, 1997), support this possibility. The involvement of CP in iron uptake by brain neurons might be more important physiologically than its role in iron efflux (Qian and Ke, 2001).…”

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confidence: 99%

Effects of development and iron status on ceruloplasmin expression in rat brain

Chang

Qian

Wang

et al. 2005

Journal Cellular Physiology

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The increased iron content in the brain of subjects with aceruloplasminemia has implicated ceruloplasmin (CP) as a major factor in the regulation of regional brain iron content. In this study, we investigated the effects of age and iron on CP expression in rat brain. In all four regions, the iron concentrations increased with developmental age. There is a similar trend in age-induced changes in CP mRNA and protein. The CP mRNA and protein levels were both lowest at postnatal day (PND) 7. The expression increased gradually with age, reaching the highest at PND196 in the striatum and substantia nigra, and at PND21 and PND63 in the cortex and hippocampus, respectively. This suggests the existence of an age-dependent pre-transcriptional regulation and a regionally specific effect of age on CP expression in the brain. Although total iron in all four regions was significantly lower in the rats fed with a low-iron diet for 6 weeks and higher in the rats with a high-iron diet than those in the control animals, no significant between-group differences in CP mRNA and protein were found in these animals, except in the substantia nigra where a significant increase in CP protein in high-iron rats was observed, and the reverse in low-iron rats. These findings suggested that the effects of iron on CP expression in the brain may be region-specific, and that regulation of CP expression by iron in the substantia nigra was at the post-transcriptional level.

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“…In contrast, Cp has been shown to mediate inward iron flux as well in some cell culture systems. Cp increases iron uptake by iron-deficient cells of hepatic and erythroid origin (22,23), and by glioblastoma cells (24,25); the Cp homologue fet3p has a similar role in high affinity iron uptake in Saccharomyces cerevisiae (26,27).…”

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confidence: 99%

Dual Role of Insulin in Transcriptional Regulation of the Acute Phase Reactant Ceruloplasmin

Seshadri¹,

Fox²,

Mukhopadhyay³

2002

Journal of Biological Chemistry

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Insulin is a potent negative regulator of the response of hepatic cells to pro-inflammatory cytokines, particularly, interleukin (IL)-6. The action of insulin is targetselective because it inhibits transcription of most but not all acute phase genes. We here show that ceruloplasmin (Cp), an acute phase reactant with important functions in iron homeostasis, is subject to a unique dual regulation by insulin. IL-6 increased Cp mRNA expres-

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Effects of ferroxidase activity and species on ceruloplasmin mediated iron uptake by BT325 cells

Cited by 14 publications

References 27 publications

Role of hepcidin in murine brain iron metabolism

Role of hepcidin in murine brain iron metabolism

Effects of development and iron status on ceruloplasmin expression in rat brain

Dual Role of Insulin in Transcriptional Regulation of the Acute Phase Reactant Ceruloplasmin

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