Effects of Ferrocenyl 4-(Imino)-1,4-Dihydro-quinolines on Xenopus laevis Prophase I - Arrested Oocytes: Survival and Hormonal-Induced M-Phase Entry

Abstract

Xenopus oocytes were used as cellular and molecular sentinels to assess the effects of a new class of organometallic compounds called ferrocenyl dihydroquinolines that have been developed as potential anti-cancer agents. One ferrocenyl dihydroquinoline compound exerted deleterious effects on oocyte survival after 48 h of incubation at 100 μM. Two ferrocenyl dihydroquinoline compounds had an inhibitory effect on the resumption of progesterone induced oocyte meiosis, compared to controls without ferrocenyl groups. In these inhibited oocytes, no MPF (Cdk1/cyclin B) activity was detected by western blot analysis as shown by the lack of phosphorylation of histone H3. The dephosphorylation of the inhibitory Y15 residue of Cdk1 occurred but cyclin B was degraded. Moreover, two apoptotic death markers, the active caspase 3 and the phosphorylated histone H2, were detected. Only 7-chloro-1-ferrocenylmethyl-4-(phenylylimino)-1,4-dihydroquinoline (8) did not show any toxicity and allowed the assembly of a histologically normal metaphase II meiotic spindle while inhibiting the proliferation of cancer cell lines with a low IC50, suggesting that this compound appears suitable as an antimitotic agent.

Inflammatory signaling pathways in the treatment of Alzheimer's disease with inhibitors, natural products and metabolites (Review)

“…Quinoline, a heterocyclic aromatic organic compound, has attracted considerable attention for its antibacterial properties ( 154 ) and its ability to inhibit amyloid aggregation ( 155 , 156 ). Consequently, this framework is widely utilized in the research and design of innovative anti-inflammatory drugs.…”

Synthesis and Biological Activity of a New Indenoisoquinoline Copper Derivative as a Topoisomerase I Inhibitor

“…WN198's IC 50 was smaller compared to cisplatin but higher than that of WN197, a copper complex and a topoisomerase inhibitor [15]. Additionally, we tested the ability of WN198 to alter Xenopus oocyte maturation and embryonic development, two useful single and highly organized cell systems to test metals and chemical effects [20][21][22][23]. Xenopus oocytes undergo a G2/M transition, after progesterone stimulation and concomitant microinjection and balneation with WN198 (0.5, 1, and 5 µM).…”

“…The appearance of a white spot, resulting from the migration of the germinal vesicle at the apex of the pigmented hemisphere of the oocyte, indicated the meiosis process or G2/M transition. Oocytes arrested in prophase I progress to metaphase II and arrest [22,23,62].…”

Cell Rover—a miniaturized magnetostrictive antenna for wireless operation inside living cells

“…It can be seen that the cell membrane has successfully healed after injection and the cell cytoplasm doesn't leak due to the injection of the antenna. We further checked the cell viability after injection by observing the appearance of the two hemispheres of the cell membrane using optical imaging which is a standard viability assay for oocytes [30][31][32][33] , for 84 h as shown in Supplementary Fig. 11, and the cells were found to be viable (Supplementary Note 8).…”