Effects of fermentation conditions on the potential anti‐hypertensive peptides released from yogurt fermented by <i>Lactobacillus helveticus</i> and Flavourzyme<sup>®</sup>

Shi, Min; Ahtesh, Fatah B; Mathai, Michael L.; McAinch, Andrew J.; Su, Xiaolong

doi:10.1111/ijfs.13253

Cited by 19 publications

(4 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the research of Villegas et al, 2014, Lb. delbrueckii subsp. lactis CRL 58 grew well at 40 °C, others grow at 37 °C (Georgalaki et al 2017;Shu et al 2018) and 42 °C (Shi et al 2017). The pH during fermentation is also a concern.…”

Section: Identification Of Peptides Generated From Goat Milk Fermented By Lb Delbrueckii Ssp Delbrueckii Bd7 In the <3 Kda Fractionmentioning

confidence: 99%

Peptide profiling of goat milk fermented by Lactobacillus delbrueckii ssp. delbrueckii BD7: Identification of potential biological activity

et al. 2021

View full text Add to dashboard Cite

Abstract. Rubak YT, Nuraida L, Iswantini D, Prangdimurti E, Sanam MUE. 2021. Peptide profiling of goat milk fermented by Lactobacillus delbrueckii ssp. delbrueckii BD7: Identification of potential biological activity. Biodiversitas 22: 3136-3145. This study investigated the angiotensin-converting enzyme (ACE) inhibitory activity in fermented goat milk by Lactobacillus delbrueckii ssp. delbrueckii BD7, characterizing the peptide and its potential as a bioactive peptide. The starter culture (2%) was inoculated into pasteurized goat skim milk (11%), then incubated at 37 °C until it reached pH 4.6. Centrifugation at 6000 g x 10 minutes at 4 °C was applied. The supernatant obtained was then ultrafiltrated using a membrane cut-off with a molecular weight of 3 kDa, and the fraction obtained was analyzed to determine the inhibitory activity of ACE. Peptides were characterized using Nano LC / MS / MS, and identification as bioactive peptides was carried out based on a literature review. ACE inhibitory activity of fermented goat milk of Lb. delbrueckii ssp. delbrueckii BD7 was 55.98 ± 3.53%. A total of 157 peptides were released with molecular weights ranging from 770.78 - 2081.12 Da and having 7-19 amino acid residues. The main peptide was hydrolyzed from casein (72.6%), cleavage in the parent protein, specific for aliphatic and aromatic amino acids. Identification of bioactive peptides based on the similarity of amino acid residues at C-terminal obtained 28 ACE inhibitor peptides, 19 antioxidant peptides, and ten antimicrobial peptides. Some of these peptides have homologous sequences with previously reported peptides. Lb. delbrueckii ssp. delbrueckii BD7 has the potential as a starter culture to produce fermented milk, which is rich in biological activity.

show abstract

Section: Identification Of Peptides Generated From Goat Milk Fermented By Lb Delbrueckii Ssp Delbrueckii Bd7 In the <3 Kda Fractionmentioning

confidence: 99%

Peptide profiling of goat milk fermented by Lactobacillus delbrueckii ssp. delbrueckii BD7: Identification of potential biological activity

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Отмечается способность отдельных видов и штаммов к высвобождению БАП с антигипертензивным, антиоксидантным, антидиабетическим и др. биологически активным действием из структуры белка Shi et al, 2016;Worsztynowicz et al, 2020;Hidalgo et al, 2015). Ферменты, сыграв ключевую роль в создании продуктов нового поколения (молоко A2, гипоаллергенные смеси, низколактозные и безлактозные продукты), еще несколько десятилетий назад стали предметом активных исследований.…”

Section: Introductionunclassified

Enzymatic Method of Bioactive Peptides Production from Milk Protein Raw Materials: A Scoping Review

Kruchinin,

Bolshakova

2023

View full text Add to dashboard Cite

Introduction: For 14 years, interest in the use of dairy raw materials for the production of bioactive peptides (BPs) with antihypertensive, antioxidant and antidiabetic effects has been in a phase of rapid growth because of the need to prevent different diseases. Particular attention is being paid to enzymatic production method, as it allows for a targeted process of BP extraction with specified properties.The purpose of this scoping review was to summarize and systematize the experience of international research over the last 14 years in the field of FG as a method to extract BPs from milk protein substrates.Materials and Methods: This scoping review was performed in accordance with the PRISMA-ScR guidelines. Scientific papers were searched in the Google Scholar database, because allows searching for scientific sources without restrictions on their format and type of scientific data presentation (book, dissertation, article), as well as includes scientific sources from other databases and performs relevance sorting.Results: The search identified 72 sources published between 2007 and 2021 in English. Included sources are devoted to hydrolysis by starter bacteria enzymes (SBE) - 50% and by commercial enzyme preparations (CEP) - 50%.Conclusion: There are a number of challenges that need to be addressed in order to increase the level of commercialization of BP production through directed hydrolysis. Creation of databases; building up the research base on co-fermentation by microorganisms and commercial enzymes; conducting PCR studies of microbial protease genes will increase the practical applicability of research and the validity of conducting more evidence-based in vivo and clinical studies.

show abstract

“…Angiotensin converting enzyme inhibitory (ACE-I) peptides from the enzymatic hydrolysis of milk proteins are able to reduce the blood pressure in rat models of hypertension [7][8][9]. Several ACE-I peptides have been demonstrated as products of enzymatic hydrolysis of milk proteins which are able to inhibit ACE activity in vitro [8,[10][11][12][13][14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Identification and Effects of Skim Milk-Derived Bioactive Antihypertensive Peptides

et al. 2021

Self Cite

View full text Add to dashboard Cite

Bioactive peptides are generated during milk fermentation or enzymatic hydrolysis. Lactobacillus (L) helveticus is commonly used to produce some types of fermented milk products. Fermented milk derived bioactive peptides are known to be beneficial in human health. Anti-hypertensive peptides play a dual role in the regulation of hypertension through the production of the vasoconstrictor angiotensin II and its inactivation of the vasodilator bradykinin. MALDI MS/MS, nano-LC/MS/MS and RP-HPLC were used to isolate peptides showing angiotensin converting enzyme inhibition (ACE-I) from 12% fermented skim milk using a combination of L. helveticus and Flavourzyme®. The fermentation procedure facilitated the identification of 133 anti-hypertensive peptides and 75% short chain amino acids, and the three with the highest ACE-I activity reduced blood pressure in a rat model of hypertension. The freeze- dried extract was supplemented in rodent chow. In this study 14-week-old male spontaneously hypertensive rats were fed for 10 weeks with the identified peptides added to chow and compared to controls supplemented with skim milk powder. Blood pressure (BP) decreased significantly (p < 0.05) from 6 to 10 weeks of FS groups (120/65 mmHg) compared with the NFS control groups, where the BP increased significantly (220/150 mmHg) (p < 0.05). The F6 fraction provided bioactive peptides with stronger antihypertensive properties than other fractions. Skim milk fermented by L. helveticus and Flavourzyme® generates several bioactive peptides which have a blood pressure lowering effect in hypertensive disease.

show abstract

Effects of fermentation conditions on the potential anti‐hypertensive peptides released from yogurt fermented by Lactobacillus helveticus and Flavourzyme^®

Cited by 19 publications

References 32 publications

Peptide profiling of goat milk fermented by Lactobacillus delbrueckii ssp. delbrueckii BD7: Identification of potential biological activity

Peptide profiling of goat milk fermented by Lactobacillus delbrueckii ssp. delbrueckii BD7: Identification of potential biological activity

Enzymatic Method of Bioactive Peptides Production from Milk Protein Raw Materials: A Scoping Review

Identification and Effects of Skim Milk-Derived Bioactive Antihypertensive Peptides

Contact Info

Product

Resources

About

Effects of fermentation conditions on the potential anti‐hypertensive peptides released from yogurt fermented by Lactobacillus helveticus and Flavourzyme®

Cited by 19 publications

References 32 publications

Peptide profiling of goat milk fermented by Lactobacillus delbrueckii ssp. delbrueckii BD7: Identification of potential biological activity

Peptide profiling of goat milk fermented by Lactobacillus delbrueckii ssp. delbrueckii BD7: Identification of potential biological activity

Enzymatic Method of Bioactive Peptides Production from Milk Protein Raw Materials: A Scoping Review

Identification and Effects of Skim Milk-Derived Bioactive Antihypertensive Peptides

Contact Info

Product

Resources

About

Effects of fermentation conditions on the potential anti‐hypertensive peptides released from yogurt fermented by Lactobacillus helveticus and Flavourzyme^®