Effects of Fengliao-Changweikang in Diarrhea-predominant Irritable Bowel Syndrome Rats and Its Mechanism Involving Colonic Motility

Jia, Mengdi; Lu, Xiufen; Wang, Zhengfang; Zhao, Luqing; Zhang, Shengsheng

doi:10.5056/jnm17093

Cited by 13 publications

(6 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The model of post-inflammatory IBS used in our study is considered to be a model of IBS-D [ 45 , 46 ] where colonic transit is generally accelerated compared to IBS-C with slow gut transit [ 1 , 47 , 48 ]. Indeed, in our model of IBS we found acceleration of the colon transit which was accompanied by an increased amplitude and frequency of spontaneous contractions of proximal colon preparations, similar to previous data by Jia et al [ 49 ]. Cellular mechanisms responsible for increased colonic motility in IBS may include up-regulation of L-type calcium channels in colonic smooth muscle and/or an increased number of interstitial cells of Cajal (ICC) due to the expression of 5-HT2B receptors [ 25 , 50 ].…”

Section: Discussionsupporting

confidence: 91%

Short chain fatty acids and colon motility in a mouse model of irritable bowel syndrome

et al. 2021

View full text Add to dashboard Cite

Background Irritable bowel syndrome (IBS) is defined as a multifactorial disorder associated with visceral hypersensitivity, altered gut motility and dysfunction of the brain-gut axis. Gut microbiota and its metabolites are proposed as possible etiological factors of IBS. Short chain fatty acids (SCFAs) induce both inhibitory and stimulatory action on colon motility, however, their effects on the IBS model were not investigated. The aim of our study was to investigate the level of SFCAs in feces and their effects on colon motility in a mouse model of IBS. Methods IBS model was induced in mice by intracolonic infusion of 1% acetic acid during the early postnatal period. Mice colon hypersensitivity was assessed by the threshold of the abdominal withdrawal reflex in response to colorectal distention. Colon contractility was studied using proximal colon specimens in isometric conditions. Transit rates were assessed by the pellet propulsion in the isolated colon. Concentrations of SCFAs in feces were measured using gas–liquid chromatography. Results The concentration of SCFAs in feces of IBS model mice was higher compared to the control group. Visceral sensitivity to colorectal distension and colonic transit rate were increased indicating IBS with predominant diarrhea. The frequency and amplitude of spontaneous contractions of proximal colon segments from IBS mice were higher, but carbachol induced contractions were lower compared to control. During acute application of SCFAs (sodium propionate, sodium acetate or butyric acid) dose-dependently (0.5–30 mM) decreased tonic tension, frequency and amplitude of spontaneous and carbachol-evoked contractions. In the mouse IBS group the inhibitory effects SCFAs on spontaneous and carbachol-evoked contractions were less pronounced. At the same time intraluminal administration of butyrate (5 mM) increased the transit rate in the colon of both groups, but its stimulatory effect was more pronounced in mouse IBS model group. Conclusion Our data indicate that the increased transit rate in the mouse IBS model group is associated with a disbalance of activating and inhibiting action of SCFAs due to chronically elevated SCFA levels, which may impact the pathogenesis of IBS with predominant diarrhea syndrome.

show abstract

Section: Discussionsupporting

confidence: 91%

Short chain fatty acids and colon motility in a mouse model of irritable bowel syndrome

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The main source of ACh in the gastrointestinal tract is a network of myenteric neurons that control peristalsis and muscle tone through the activation of metabotropic M 2 and M 3 -type muscarinic receptors (Gallegos-Perez et al, 2014). The spontaneous contraction of colonic longitudinal smooth muscles and their response to ACh was significantly increased in the model rats under maternal and infant separation combined with bondage stress (Jia et al, 2018). To investigate the molecular mechanism of muscle contraction dysfunction in SERT-KO rats in detail, we examined the expression of the muscarinic receptors, including M 3 R and M 2 R, in colon tissues.…”

mentioning

confidence: 99%

Shugan Decoction Alleviates Colonic Dysmotility in Female SERT-Knockout Rats by Decreasing M3 Receptor Expression

et al. 2020

View full text Add to dashboard Cite

Background: Irritable bowel syndrome (IBS) is a functional gut disease characterized by visceral hypersensitivity and gut motor dysfunction. Serotonin (5-hydroxytryptamine, 5-HT) is an important enteric neurotransmitter. High levels of 5-HT aggravate IBS symptoms. The serotonin reuptake transporter (SERT) is a membrane-embedded transporter involved in IBS pathogenesis that plays an important role in regulating 5-HT signaling. Aim: We investigated whether gut motor function was altered in SERT-knockout (SERT-KO) rats. Additionally, we sought to determine whether Shugan decoction (SGD), a clinically experienced prescription for the treatment of IBS, exerts regulatory effects on intestinal motility in SERT-KO rats, and attempted to identify the mechanisms involved. Method: SERT-KO rats were produced by transcription activator-like effector nuclease (TALEN) technology. Fecal pellet output was measured for ten consecutive days to estimate distal colonic motility. Small intestinal motility was measured by charcoal-meal experiments. The colonic and small intestinal muscle contractile activities were measured by organ bath study. Western blot was used to analyze the muscarinic receptor expression in colon tissue.

show abstract

“…Stress animal models present similar pathophysiologic abnormalities to that in IBS-D patients. Therefore, we used NMS + RS-treated rats to establish the IBS-D rat model [ 18 , 23 ].…”

Section: Discussionmentioning

confidence: 99%

“…The IBS-D model was induced as follows [ 17 , 18 ]. On postnatal days 4–21, the neonatal maternal separation (NMS) was conducted.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Tongxie Anchang Decoction Relieves Visceral Hypersensitivity in Diarrhea-Predominant Irritable Bowel Syndrome Rats by Regulating the NGF/TrkA Signaling Pathway

Tan

Pei

Xie

et al. 2021

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Irritable bowel syndrome (IBS) is a functional gastrointestinal disease characterized by visceral hypersensitivity-related abdominal pain, in which diarrhea-predominant IBS (IBS-D) is the main subtype and has a high clinical incidence. Tongxie Anchang Decoction (TXACD) has been proved to significantly improve abdominal pain in patients with IBS-D, but its underlying therapeutic mechanism still remains unclear. In the present study, IBS-D model rats were induced by neonatal maternal separation (NMS) combined with restraint stress (RS). The therapeutic effect of TXACD was evaluated by fecal characteristics and abdominal withdrawal reflex (AWR) scores. After 14 days of intragastric administration, the colonic tissues of rats were collected to detect the protein and gene level of the NGF, TrkA, and TRPV1 using Western blotting and real-time polymerase chain reaction, respectively, and detect mast cells infiltration using toluidine blue staining. The abdominal aorta blood centrifuged was collected for detecting serum levels of SP, 5-HT, and CGRP with ELISA. The results revealed that TXACD could significantly improve visceral hypersensitivity in IBS-D rats, reflected in the decrease of AWR score and the serum levels of SP, 5-HT, and CGRP. In addition, TXACD treatment could alleviate mast cells infiltration. Moreover, the expression levels of the NGF, TrkA, and TRPV1 were repressed by TXACD. The findings of the present study indicated that the therapeutic effect of TXACD on visceral hypersensitivity might be closely related to the downregulation of the NGF/TrkA signaling pathway, the reversal of TRPV1 expression and mast cells infiltration, and the decreased release of neuroendocrine factors SP, 5-HT, and CGRP.

show abstract

Effects of Fengliao-Changweikang in Diarrhea-predominant Irritable Bowel Syndrome Rats and Its Mechanism Involving Colonic Motility

Cited by 13 publications

References 46 publications

Short chain fatty acids and colon motility in a mouse model of irritable bowel syndrome

Short chain fatty acids and colon motility in a mouse model of irritable bowel syndrome

Shugan Decoction Alleviates Colonic Dysmotility in Female SERT-Knockout Rats by Decreasing M3 Receptor Expression

Tongxie Anchang Decoction Relieves Visceral Hypersensitivity in Diarrhea-Predominant Irritable Bowel Syndrome Rats by Regulating the NGF/TrkA Signaling Pathway

Contact Info

Product

Resources

About