Effects of feeding transgenic corn with mCry1Ac or maroACC gene to laying hens for 12 weeks on growth, egg quality and organ health

Zhong, Ruqing; Chen, L.; Gao, Le; Zhang, L. L.; Yao, Baojin; Yang, Xiaoguang; Zhang, H. F.

doi:10.1017/s1751731116000203

Cited by 6 publications

(7 citation statements)

References 22 publications

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“…The visceral organ is the most important of the functional organs for poultry growth and development, and a well‐developed internal organ was demonstrated to be essential for the absorption of nutrients . The present results show that the relative weight of the heart and kidney were increased in number or significance by 15 mg kg −1 PGZ supplementation, indicating that this compound may be one way to increase the utilization of nutrients .…”

Section: Discussionmentioning

confidence: 50%

Dietary supplementation with pioglitazone hydrochloride improves intramuscular fat, fatty acid profile, and antioxidant ability of thigh muscle in yellow‐feathered chickens

et al. 2019

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BACKGROUND Muscle fat content and fatty acid composition play an important role in poultry flavor and taste. To investigate the effects of pioglitazone hydrochloride (PGZ) on growth performance and thigh muscle quality in yellow‐feathered chickens, 360 female chickens were randomly divided into three groups and treated with three doses of PGZ (0, 7.5, and 15 mg kg−1) for 28 days. Each group had six replicates of 20 chickens. RESULTS The results showed that dietary supplementation with 15 mg kg−1 PGZ increased average daily feed intake (ADFI) and the average daily gain (ADG) from 0 to 14 days. Furthermore, the triglyceride (TG) level was decreased by 15 mg kg−1 PGZ, whereas the eviscerated yield was increased. The relative weight of the heart and kidneys showed a linear increase with dietary PGZ supplementation, and the drip loss of the thigh muscle was significantly decreased by 15 mg kg−1 PGZ supplementation. Moreover, a* value, intramuscular fat (IMF), and polyunsaturated fatty acids (PUFAs) showed a linear increase, and pH24 h and drip loss showed a quadratic influence with the levels of PGZ supplementation. In particular, the PUFA proportion was increased by 7.63% and 9.14% in the 7.5 mg kg−1 PGZ and 15 mg kg−1 PGZ groups, respectively. Additionally, 15 mg kg−1 of PGZ increased the total antioxidant capacity (T‐AOC) and glutathione peroxidase (GSH‐PX) activity. CONCLUSION In summary, 15 mg kg−1 PGZ has substantial effects on growth performance and meat quality, particularly by decreasing drip loss and increasing IMF content, PUFA proportions, and antioxidant ability. © 2019 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 50%

Dietary supplementation with pioglitazone hydrochloride improves intramuscular fat, fatty acid profile, and antioxidant ability of thigh muscle in yellow‐feathered chickens

et al. 2019

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“…All diets were formulated to meet or exceed the nutrient requirements for poultry (Ministry of Agriculture of PR China, 2004; National Research Council, 1994) as a guideline (see Supporting information, Table S1). 19 Diets were mixed in the order CT diet, CC diet and BT diet to minimize the potential for cross‐contamination of nontransgenic sources with transgenic sources. The existence of the mCry1Ac and maroACC genes and the mCry1Ac and maroACC proteins was detected by the polymerase chain reaction (PCR) method and antibody‐specific enzyme‐linked immunosorbent assay method, respectively.…”

Section: Methodsmentioning

confidence: 99%

Modulation of cecal microbiota in laying hens via intake of genetically modified corn with the maroACC or mCry1Ac genes

et al. 2020

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BACKGROUND: The present study investigated the chronic effect on the composition and proportions of the cecal microbiota of laying hens for 12 weeks after consuming two genetically modified (GM) corns containing the maroACC gene from the Agrobacterium tumefaciens strain (CC) and the mCry1Ac gene from the Bacillus thuringiensis strain (BT) in comparison with the isogenic corn (CT). RESULTS: In total, 72 hens were randomly assigned to the CT corn-based diet, CC corn-based diet and BT corn-based diet. The absolute weights of abdominal fat, breast, thigh meat and organ weight were not affected by the dietary treatment. Highthroughput 16S rRNA gene sequencing revealed a few differences in the composition of cecal microbiota among the treatments. The only difference with respect to bacterial family was that the cecal abundance of Porphyromonadaceae (3.46 versus 2.11%; P = 0.073) tended to be higher for birds consuming the CC diet than those birds consuming the CT diet. Birds fed the BT diet tended to have a higher abundance of Barnesiella (0.62 versus 0.13%; P = 0.057) and a lower abundance of unclassified Ruminococcaceae (0.64 versus 1.19%; P = 0.097) than those fed the CT diet. Considering beneficial intestinal Barnesiella, this decreases and ultimately clears the colonization of vancomycin-resistant Enterococcus. The unclassified Ruminococcaceae was a low-frequency and low-abundance bacterial taxa and was not associated with intestinal pathology. CONCLUSION: These results indicate a similar modulation of cecal microbiota in laying hens by long-term feeding among transgenic CC corn, BT corn and non-transgenic corn and provide data for biosafety evaluation of the transgenic corn.

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“…Most of the GM maize that is cultivated in the world is insect resistant, herbicide tolerance, or a combination of both traits. Two transgenic maizes are currently under development in China [9]. A transgenic maize line was produced by the insertion of the mCry1Ac gene that was derived from Bacillus thuringiensis strain (BT) and transcription of the mCry1Ac gene confers resistance to insect damage [9].…”

Section: Introductionmentioning

confidence: 99%

“…Two transgenic maizes are currently under development in China [9]. A transgenic maize line was produced by the insertion of the mCry1Ac gene that was derived from Bacillus thuringiensis strain (BT) and transcription of the mCry1Ac gene confers resistance to insect damage [9]. Another transgenic maize line was produced by the insertion of the maroACC gene derived from the Agrobacterium tumefaciens strain (CC) and a gene shuffling process to optimize the kinetics of glyphosate acetyltransferase activity for acetylating the herbicide glyphosate functionally improved the maroACC gene [9].…”

Section: Introductionmentioning

confidence: 99%

“…A transgenic maize line was produced by the insertion of the mCry1Ac gene that was derived from Bacillus thuringiensis strain (BT) and transcription of the mCry1Ac gene confers resistance to insect damage [9]. Another transgenic maize line was produced by the insertion of the maroACC gene derived from the Agrobacterium tumefaciens strain (CC) and a gene shuffling process to optimize the kinetics of glyphosate acetyltransferase activity for acetylating the herbicide glyphosate functionally improved the maroACC gene [9]. The development of transgenic maize provides growers with such benefits [10], but its presence of food and feed has been the focus of attention that is related to potential health risks.…”

Section: Introductionmentioning

confidence: 99%

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The Chronic Effect of Transgenic Maize Line with mCry1Ac or maroACC Gene on Ileal Microbiota Using a Hen Model

2019

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The experiment was to determine the chronic effects of two transgenic maize lines that contained the mCry1Ac gene from the Bacillus thuringiensis strain (BT) and the maroACC gene from Agrobacterium tumefaciens strain (CC), respectively, on ileal microbiota of laying hens. Seventy-two laying hens were randomly assigned to one of the three dietary treatments for 12 weeks, as follows: (1) nontransgenic near-isoline maize-based diet (CT diet), (2) BT maize-based diet (BT diet), and (3) CC maize-based diet (CC diet). Ileum histological examination did not indicate a chronic effect of two transgenic maize diets. Few differences were observed in any bacterial taxa among the treatments that used high-throughput 16S rRNA gene sequencing. The only differences that were observed for bacterial genera were that Bifidobacterium belong within the Bifidobacteriaceae family tended to be greater (p = 0.114) abundant in hens fed the transgenic maize-based diet than in hens fed the CT diet. Birds that consumed the CC maize diet tended to have less abundance (p = 0.135) of Enterobacteriaceae family in the ileum than those that consumed the CT maize diet. These results indicate the lack of adverse effects of the BT maize and the CC maize lines on the ileal microbiota of hens for long term and provide important data regarding biosafety assessment of the transgenic maize lines.

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Effects of feeding transgenic corn with mCry1Ac or maroACC gene to laying hens for 12 weeks on growth, egg quality and organ health

Cited by 6 publications

References 22 publications

Dietary supplementation with pioglitazone hydrochloride improves intramuscular fat, fatty acid profile, and antioxidant ability of thigh muscle in yellow‐feathered chickens

Dietary supplementation with pioglitazone hydrochloride improves intramuscular fat, fatty acid profile, and antioxidant ability of thigh muscle in yellow‐feathered chickens

Modulation of cecal microbiota in laying hens via intake of genetically modified corn with the maroACC or mCry1Ac genes

The Chronic Effect of Transgenic Maize Line with mCry1Ac or maroACC Gene on Ileal Microbiota Using a Hen Model

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