Effects of feeding status on nucb1 and nucb2A mRNA expression in the hypothalamus of Schizothorax davidi

Yuan, Dengyue; Zhang, Xin; Wang, Bin; Tang, Tao; Luo, Lei; Deng, Xingxing; Zhou, Chaowei; Li, Zhiqiong

doi:10.1007/s10695-020-00780-1

Cited by 2 publications

(1 citation statement)

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“…In response to feeding, crh and gnrh2 have been recently reported to have similar expression patterns in Schizothorax davidi (another Cypriniformes species) (Yuan et al 2021 ). However, other anorexigenic genes were also found showing strong response to feeding, which can be independent of leptin mediated transcriptional regulation (Yuan et al 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptional study reveals a potential leptin-dependent gene regulatory network in zebrafish brain

Ahi

Tsakoumis

Brunel

et al. 2021

Fish Physiol Biochem

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The signal mediated by leptin hormone and its receptor is a major regulator of body weight, food intake and metabolism. In mammals and many teleost fish species, leptin has an anorexigenic role and inhibits food intake by influencing the appetite centres in the hypothalamus. However, the regulatory connections between leptin and downstream genes mediating its appetite-regulating effects are still not fully explored in teleost fish. In this study, we used a loss of function leptin receptor zebrafish mutant and real-time quantitative PCR to assess brain expression patterns of several previously identified anorexigenic genes downstream of leptin signal under different feeding conditions (normal feeding, 7-day fasting, 2 and 6-h refeeding). These downstream factors include members of cart genes, crhb and gnrh2, as well as selected genes co-expressed with them based on a zebrafish co-expression database. Here, we found a potential gene expression network (GRN) comprising the abovementioned genes by a stepwise approach of identifying co-expression modules and predicting their upstream regulators. Among the transcription factors (TFs) predicted as potential upstream regulators of this GRN, we found expression pattern of sp3a to be correlated with transcriptional changes of the downstream gene network. Interestingly, the expression and transcriptional activity of Sp3 orthologous gene in mammals have already been implicated to be under the influence of leptin signal. These findings suggest a potentially conserved regulatory connection between leptin and sp3a, which is predicted to act as a transcriptional driver of a downstream gene network in the zebrafish brain.

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Section: Discussionmentioning

confidence: 99%