Effects of Eyestalk Ablation at Different Times of the Year on Molting and Reproduction of the Estuarine Grapsid Crab Chasmagnathus Granulata (Decapoda, Brachyura)

Stella, Vivian S.; Greco, Laura S. López; Rodríguez, Enrique M.

doi:10.1163/20021975-99990036

Cited by 8 publications

(7 citation statements)

References 15 publications

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“…adult females spawn only during spring and summer. During the postreproductive period (autumn), the ovary is quiescent; however, a significant proportion of adult females continue to molt during this period (López Greco & Rodríguez 1999;Stella et al 2000). N. granulata is an important species because several fish species feed on it and because it serves as a model species for several physiological and ecological research lines (Menni 1983;Sánchez et al 1991).…”

Section: Introductionmentioning

confidence: 99%

Induction of vitellogenesis by 17-hydroxyprogesterone and methyl farnesoate during post-reproductive period, in the estuarine crabNeohelice granulata

Medesani

Ferré

Canosa

et al. 2015

Invertebrate Reproduction & Development

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Adult female crabs (Neohelice granulata) were treated during the post-reproductive period with both 17-hydroxyprogesterone (17PG) and methyl farnesoate (MF). During the 4-week in vivo assay, animals were fed hormone-enriched (17PG or MF) pellets twice a week, at a dose of 2 nmol/g of body weight. Additionally, at the middle of the in vivo experiment, ovarian explants were incubated in vitro with each hormone at a concentration of 15 μM for 24 h. At the end of the in vivo assay, both the gonadosomatic index and the vitellogenic protein content (Vg) of crabs treated with either 17PG or MF were significantly higher (p < 0.05) than the values of the concurrent controls. In addition, the hemolymphatic vitellogenin level of both hormonally treated groups was higher. At the end of the in vitro assay, ovary explants incubated with 17PG had a significantly higher (p < 0.05) protein synthesis and content of Vg proteins than controls. Ovary explants incubated with MF had similar levels to controls (p > 0.05). The involvement of these hormones in the regulation of ovarian growth during the reproductive cycle of N. granulata is discussed.

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Section: Introductionmentioning

confidence: 99%

Induction of vitellogenesis by 17-hydroxyprogesterone and methyl farnesoate during post-reproductive period, in the estuarine crabNeohelice granulata

Medesani

Ferré

Canosa

et al. 2015

Invertebrate Reproduction & Development

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“…This means that many matured eggs were still retained at various developmental stages in the gonad, because the increase in R/D could be interpreted as the growing and deposit of egg protein per oocyte. Stella et al (2000) found that unilateral eyestalk ablation of grapsid crabs did not cause significant effects on moulting in any case. On the contrary, about 33% of the unilaterally ablated females spawned twice during the reproductive period.…”

Section: Discussionmentioning

confidence: 74%

The effects of manipulating water temperature, photoperiod, and eyestalk ablation on gonad maturation of the swimming crab, Portunus trituberculatus

Jang¹,

Kim²,

Seo³

et al. 2010

Crustac

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An experiment to induce maturation and spawning of Portunus trituberculatus by manipulating seawater temperature, photoperiod, and eyestalk ablation, and to study the effect these factors have on the variation of nucleic acid content in gonadal tissue, was conducted. Non-incubating adult females were divided among three temperature treatments (natural, 10 • C, and 20 • C) and two photoperiod treatments (15 h and 9 h daylight periods per day, respectively). Within each temperature-photoperiod combination, subjects were further divided into each of two groups, i.e., unilaterally eyestalk ablated and unablated. The gonadosomatic index, spawning rate, and nucleic acid variation were examined for each treatment at 3-week intervals. Holding ablated females at 20 • C and providing 15 h of light photoperiod had the most profound effect on the induction of ovarian maturation and spawning. Gonad maturation also occurred in the unablated, 20 • C, 15 h light group, although to a lower extent. Gonad maturation did not take place for groups exposed to lower temperatures, despite either ablation or photoperiod treatments.RNA to DNA ratios were strongly correlated to the state of gonad maturation, with the highest values obtained for the ablated groups held at temperatures under 20 • C for the 15 h light photoperiod. Under these conditions, increasing DNA concentration, reflecting gonad differentiation (small cell size and a large number of cells per unit of tissue), began three weeks earlier than for the other treatments. These results suggest that P. trituberculatus requires not only a raised temperature but also another stimulus, such as eyestalk ablation or a long photoperiod, to induce off-season maturation and spawning.

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“…Eyestalk ablation essentially removes the XO-SG complex, thus stopping or suppressing the production of MIH and allowing ecdysteroids produced by the Y-organ to act (Stella et al, 2000).…”

Section: Eyestalk Ablationmentioning

confidence: 99%

“…It was found that crabs subjected to eyestalk ablation at intermoult stage exhibited shorter intermoult duration compared with those that had their eyestalk ablated during postmoult stage or without eyestalk ablation (Rana, 2018;Sukardi et al, 2019). Also, bilateral eyestalk ablation induced faster moulting and resulted in higher moulting rate compared with unilateral ablation (Stella et al, 2000).…”

Section: Eyestalk Ablationmentioning

confidence: 99%

“…The ability to induce moulting is obvious in most moult induction methods although different methods use different approaches. ecdysteroids (Qiau et al, 2018;Stella et al, 2000). The administration of ecdysteroid and phytoecdysteroid into crab directly increase its ecdysteroid level and accelerate the moulting process (Fujaya, 2011;Raghavan & Ayanath, 2019).…”

Section: Comparison B E T Ween Moult Induc Tion Me Thodsmentioning

confidence: 99%

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Moult induction methods in soft‐shell crab production

Waiho

Ikhwanuddin

Baylon

et al. 2021

Aquaculture Research

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Soft‐shell crabs are gaining attention internationally as a more lucrative option of selling commercially important portunid species due to their ease of consumption, high nutritional values, and unique and excellent taste. This product, however, is only attainable in captivity as crabs are harvested right after moulting when their exoskeletons are still soft. Among the most crucial factor in soft‐shell crab production is the moult induction method. Shorter moult intervals imply more soft‐shell crabs could be produced, increasing productivity and profit for farmers. This review describes the moulting event, soft‐shell crab production process and production systems, and highlights the currently available and potential moult induction methods, including eyestalk ablation, limb autotomy, the use of ecdysteroid, phytoecdysteroid, biogenic amines and methyl farnesoate, the inhibition of moult‐inhibiting hormone (MIH) and the regulation of physical parameters. This review further compares these moult induction methods and their benefits towards soft‐shell crab production.

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Effects of Eyestalk Ablation at Different Times of the Year on Molting and Reproduction of the Estuarine Grapsid Crab Chasmagnathus Granulata (Decapoda, Brachyura)

Cited by 8 publications

References 15 publications

Induction of vitellogenesis by 17-hydroxyprogesterone and methyl farnesoate during post-reproductive period, in the estuarine crabNeohelice granulata

Induction of vitellogenesis by 17-hydroxyprogesterone and methyl farnesoate during post-reproductive period, in the estuarine crabNeohelice granulata

The effects of manipulating water temperature, photoperiod, and eyestalk ablation on gonad maturation of the swimming crab, Portunus trituberculatus

Moult induction methods in soft‐shell crab production

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