Effects of Extracts from<i>Trifolium medium</i>L. and<i>Trifolium pratense</i>L. on Development of Estrogen Deficiency-Induced Osteoporosis in Rats

Cegieła, Urszula; Folwarczna, Joanna; Pytlik, Maria; Zgórka, Grażyna

doi:10.1155/2012/921684

Cited by 25 publications

(31 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Bone mineralization was decreased. The changes led to worsening of mechanical properties of cancellous bone of the tibial metaphysis, but not those of compact bone of the femoral diaphysis, consistently with most of our previous studies .…”

Section: Discussionsupporting

confidence: 90%

Favorable effect of moderate dose caffeine on the skeletal system in ovariectomized rats

Folwarczna

Pytlik

Zych

et al. 2013

Molecular Nutrition Food Res

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 90%

Favorable effect of moderate dose caffeine on the skeletal system in ovariectomized rats

Folwarczna

Pytlik

Zych

et al. 2013

Molecular Nutrition Food Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…In fact, the skeletal effects of morphine and buprenorphine in OVX rats were similar to those induced by estradiol supplementation in our previous studies (Folwarczna et al 2009; Cegieła et al 2012). Taken together, drugs acting through opioid receptors did not significantly affect the systemic estrogen levels in rats.…”

Section: Discussionsupporting

confidence: 87%

Opioid receptor agonists may favorably affect bone mechanical properties in rats with estrogen deficiency-induced osteoporosis

Janas

Folwarczna

2016

Naunyn-Schmiedeberg's Arch Pharmacol

Self Cite

View full text Add to dashboard Cite

The results of epidemiological, clinical, and in vivo and in vitro experimental studies on the effect of opioid analgesics on bone are inconsistent. The aim of the present study was to investigate the effect of morphine (an agonist of opioid receptors), buprenorphine (a partial μ opioid receptor agonist and κ opioid receptor antagonist), and naloxone (an antagonist of opioid receptors) on the skeletal system of female rats in vivo. The experiments were carried out on 3-month-old Wistar rats, divided into two groups: nonovariectomized (intact; NOVX) rats and ovariectomized (OVX) rats. The bilateral ovariectomy was performed 7 days before the start of drug administration. Morphine hydrochloride (20 mg/kg/day s.c.), buprenorphine (0.05 mg/kg/day s.c.), or naloxone hydrochloride dihydrate (2 mg/kg/day s.c.) were administered for 4 weeks to NOVX and OVX rats. In OVX rats, the use of morphine and buprenorphine counteracted the development of osteoporotic changes in the skeletal system induced by estrogen deficiency. Morphine and buprenorphine beneficially affected also the skeletal system of NOVX rats, but the effects were much weaker than those in OVX rats. Naloxone generally did not affect the rat skeletal system. The results confirmed the role of opioid receptors in the regulation of bone remodeling processes and demonstrated, in experimental conditions, that the use of opioid analgesics at moderate doses may exert beneficial effects on the skeletal system, especially in estrogen deficiency.

show abstract

“…Similar changes in the ovariectomy rat model were previously observed [30, 45–47]. Estradiol supplementation caused withdrawal of some alterations connected with the uterus and body mass.…”

Section: Discussionsupporting

confidence: 84%

Effect of Formononetin on Mechanical Properties and Chemical Composition of Bones in Rats with Ovariectomy-Induced Osteoporosis

Kaczmarczyk-Sedlak

Wojnar

Zych

et al. 2013

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Formononetin is a naturally occurring isoflavone, which can be found in low concentrations in many dietary products, but the greatest sources of this substance are Astragalus membranaceus, Trifolium pratense, Glycyrrhiza glabra, and Pueraria lobata, which all belong to Fabaceae family. Due to its structural similarity to 17β-estradiol, it can mimic estradiol's effect and therefore is considered as a “phytoestrogen.” The aim of this study was to examine the effect of formononetin on mechanical properties and chemical composition of bones in rats with ovariectomy-induced osteoporosis. 12-week-old female rats were divided into 4 groups: sham-operated, ovariectomized, ovariectomized treated with estradiol (0.2 mg/kg) and ovariectomized treated with formononetin (10 mg/kg). Analyzed substances were administered orally for 4 weeks. Ovariectomy caused osteoporotic changes, which can be observed in bone biomechanical features (decrease of maximum load and fracture load and increase of displacements for maximum and fracture loads) and bone chemical composition (increase of water and organic fraction content, while a decrease of minerals takes place). Supplementation with formononetin resulted in slightly enhanced bone mechanical properties and bone chemistry improvement (significantly lower water content and insignificantly higher mineral fraction content). To summarize, administration of formononetin to ovariectomized rats shows beneficial effect on bone biomechanical features and chemistry; thus, it can prevent osteoporosis development.

show abstract

Effects of Extracts fromTrifolium mediumL. andTrifolium pratenseL. on Development of Estrogen Deficiency-Induced Osteoporosis in Rats

Cited by 25 publications

References 59 publications

Favorable effect of moderate dose caffeine on the skeletal system in ovariectomized rats

Favorable effect of moderate dose caffeine on the skeletal system in ovariectomized rats

Opioid receptor agonists may favorably affect bone mechanical properties in rats with estrogen deficiency-induced osteoporosis

Effect of Formononetin on Mechanical Properties and Chemical Composition of Bones in Rats with Ovariectomy-Induced Osteoporosis

Contact Info

Product

Resources

About