Effects of extracts from Cordyceps sinensis on M1 muscarinic acetylcholine receptor in vitro and in vivo

Abstract

Cholinergic dysfunction is implicated in the pathogenesis of memory impairment related to Alzheimer's disease (AD). Accordingly, regulation of M1 muscarinic acetylcholine receptor (M1 mAChR) has been one of the major targets in the development of novel drugs for AD. Utilizing an in vitro system for evaluation of the M1 mAChR, we have recently identified that extracts from Cordyceps sinensis (CS) promote M1 mAChR function. In this study, we examined the effect of pretreatment with several types of CS extracts in F11 neurohybrid cells on ERK phosphorylation induced by a muscarinic agonist, carbachol (CCh). A mixed extract of a hot water extract and an ethanol extract from CS augmented ERK phosphorylation by CCh presumably through upregulation of M1 mAChR function. We further examined the effect of oral administration of CS extracts on memory impairment induced by a muscarinic antagonist scopolamine in mice, finding that CS extracts ameliorated scopolamine-induced amnesia in vivo, consistent with the in vitro data. Thus CS extracts may contribute to the prevention of memory impairment related to AD.

Scopolamine Reduces the Density of M1 Muscarinic Neurons in Rats' Hippocampus

“…We have two types of acetylcholine receptors, muscarinic and nicotinic (Chiba et al, 2010), and muscarinic acetylcholine receptors divided to five subtypes: M1, M2, M3, M4, M5 (Volpicelli & Levey, 2004;Lebois et al, 2009). Although the role of each subtype is poorly known, in the hippocampus the muscarinic receptors are related to learning and memory (Ferreira et al, 2003).…”

Medicinal Value of Cordyceps sinensis

“…Treatment with Cordyceps sinensis on memory impairment related to Alzheimer's Disease (AD) was observed by studying the role of M1 muscarinic acetylcholine receptor (M1 mAChR) on F11 neurohybrid cells [11].…”