Effects of extraction methods on the digestibility, cytotoxicity, prebiotic potential and immunomodulatory activity of taro (Colocasia esculenta) water-soluble non-starch polysaccharide

Anwar, Mylene; Mros, Sonya; McConnell, Michelle; Bekhit, Alaa El‐Din A.

doi:10.1016/j.foodhyd.2021.107068

Cited by 14 publications

(12 citation statements)

References 50 publications

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“…This is attributed to the combined beneficial effect from the digested Tc-WS-NSP and the high bacterial concentration of the probiotics. Our previous study demonstrated the ability of the digested Tc-WS-NSP to support the growth of the probiotics L. acidophilus , B. breve , and B. infantis [ 29 ]. Thus, aside from the anti-adherence capacity of the digested Tc-WS-NSP against K. oxytoca , the digested Tc-WS-NSP may have also supported the growth and viability of the probiotics.…”

Section: Discussionmentioning

confidence: 99%

“…Following the 24 h incubation period, the IL-8 produced by the TNF-α stimulated HT-29 cells in the cell culture supernatants were analysed using ELISA (IL-8 ELISA test kit, OptEIA™, BD Biosciences, San Diego, CA, USA) according to the manufacturer’s protocol as described previously [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

“…However, due to the variability of the reported efficacy of the various prophylactic agents against NEC and the need to further investigate appropriate prebiotic and probiotic dosage and synbiotic mixture combinations [ 28 ], more scientific investigations are required to explore the use of new prebiotic materials, efficient probiotics and synbiotic mixtures that can be useful as a prophylactic agent for NEC. Our previous study [ 29 ] demonstrated that taro ( Colocasia esculenta ), a tuberous root crop containing WS-NSP that can downregulate the production of IL-8 produced by tumor necrosis factor alpha (TNF-α) stimulated HT-29 cells, a gut epithelial cell line. The WS-NSP of taro corm (Tc-WS-NSP), unlike its starch, is considered a minor component [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

“…It also exhibits a number of biological activities including antidiabetic potential [ 36 ], anti-oxidative [ 31 ], antimetastatic [ 37 ], anti-inflammatory, and immunomodulatory [ 38 , 39 , 40 ] activities. In addition, it can support the growth of L. acidophilus , B. breve , and B. infantis [ 29 ], which are probiotics capable of regulating the production of pro-inflammatory cytokine IL-8. These novel findings on the potential of Tc-WS-NSP, solely or in combination with probiotics suggest its usefulness as a prophylactic agent against intestinal diseases such as NEC.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Taro (Colocasia esculenta) Water-Soluble Non-Starch Polysaccharide, Lactobacillus acidophilus, Bifidobacterium breve, Bifidobacterium infantis, and Their Synbiotic Mixtures on Pro-Inflammatory Cytokine Interleukin-8 Production

et al. 2022

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In the past decades, the regulation of pro-inflammatory cytokine production, including interleukin-8 (IL-8), has been the goal of many targeted therapeutic interventions for Necrotising enterocolitis (NEC), a gastrointestinal disease commonly associated with a very low birth weight in preterm infants. In this study, the ability to regulate the production of IL-8 of the water-soluble non-starch polysaccharide (WS-NSP) from taro corm (Tc-WS-NSP) extracted using a conventional (CE) or improved conventional (ICE) extraction method, of the probiotics Lactobacillus acidophilus, Bifidobacterium breve, and Bifidobacterium infantis, and their synbiotic mixtures were evaluated. The TNF-α stimulated HT-29 cells were incubated with undigested or digested Tc-WS-NSPs (CE or ICE), probiotics, and their synbiotic mixtures with Klebsiella oxytoca, an NEC-positive-associated pathogen. Overall, the synbiotic mixtures of digested Tc-WS-NSP-ICE and high bacterial concentrations of L. acidophilus (5.57 × 109), B. breve (2.7 × 108 CFU/mL), and B. infantis (1.53 × 108) demonstrated higher (42.0%, 45.0%, 43.1%, respectively) ability to downregulate IL-8 compared to the sole use of Tc-WS-NSPs (24.5%), or the probiotics L. acidophilus (32.3%), B. breve (37.8%), or B. infantis (33.1%). The ability demonstrated by the Tc-WS-NSPs, the probiotics, and their synbiotics mixtures to downregulate IL-8 production in the presence of an NEC-positive-associated pathogen may be useful in the development of novel prophylactic agents against NEC.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Taro (Colocasia esculenta) Water-Soluble Non-Starch Polysaccharide, Lactobacillus acidophilus, Bifidobacterium breve, Bifidobacterium infantis, and Their Synbiotic Mixtures on Pro-Inflammatory Cytokine Interleukin-8 Production

et al. 2022

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“…Usually, non-starch polysaccharide stimulate the macrophages by binding to pattern recognition receptors (PRRs) such as toll-like receptors (TLRs), Dectin-1, and complement receptor type 3 (CR3), and trigger the signal transduction pathways including phosphoinositide-3-kinase (PI3K)/Akt, mitogen-activated protein kinases (MAPKs), as well as transcription factors such as nuclear factor (NF)-κB and activator protein (AP) [7]. Numerous researches have been done to evaluate the immunomodulatory activity of non-starch polysaccharides derived from various natural resources [7,[21][22][23][24]. However, there are only a few investigations into the effect of structural changes in polysaccharide on macrophage activity.…”

Section: Introductionmentioning

confidence: 99%

Structural Elucidation and Immunostimulatory Activities of Quinoa Non-starch Polysaccharide Before and After Deproteinization

Cao

Palanisamy

et al. 2021

J Polym Environ

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Non-starch polysaccharides derived from natural resources play a significant role in the field of food science and human health due to their extensive distribution in nature and less toxicity. In this order, the immunostimulatory activity of a non-starch polysaccharide (CQNP) from Chenopodium quinoa was examined before and after deproteination in murine macrophage RAW 264.7 cells. The chemical composition of CQNP and deproteinated-CQNP (D-CQNP) were spectrometrically analysed that revealed the presence of carbohydrate (22.7 ± 0.8% and 39.5 ± 0.8%), protein (41.4 ± 0.5% and 20.8 ± 0.5%) and uronic acid (8.7 ± 0.3% and 6.7 ± 0.2%). The monosaccharide composition results exposed that CQNP possesses a high amount of arabinose (34.5 ± 0.3) followed by galactose (26.5 ± 0.2), glucose (21.9 ± 0.3), rhamnose (7.0 ± 0.1), mannose (6.0 ± 0.1) and xylose (4.2 ± 0.2). However, after deproteination, a difference was found in the order of the monosaccharide components, with galactose (41.1 ± 0.5) as a major unit followed by arabinose (34.7 ± 0.5), rhamnose (10.9 ± 0.2), glucose (6.6 ± 0.2), mannose (3.4 ± 0.2) and xylose (3.2 ± 0.2). Further, D-CQNP potentially stimulate the RAW 264.7 cells through the production of nitric oxide (NO), upregulating inducible nitric oxide synthase (iNOS) and various pro-inflammatory cytokines including interleukin (IL)-1β, IL-6, IL-10, and tumor necrosis factor-alpha (TNF-α). Moreover, stimulation of RAW 264.7 cells by D-CQNP takes place along the NF-κB and the MAPKs signaling pathways through the expression of cluster of differentiation 40 (CD40). This results demonstrate that RAW 264.7 cells are effectively stimulated after removal of the protein content in C. quinoa non-starch polysaccharides, which could be useful for develop a new immunostimulant agent.

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Functional profile and encapsulating properties of Colocasia esculenta (Taro)

Zubaır

Imran

Islam

et al. 2023

Food Science & Nutrition

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Especially in tropical and subtropical countries, tuber and root crops have developed into important food crops. Due to its use in food preparation, aesthetics, and medicine, taro (Colocasia esculenta) is ranked as the fifth most important root crop. In comparison, it stores a considerable quantity of starch – even more than potatoes, sweet potatoes, cassava, and other similar crops. Colocasia leaves are lower in calories and high in dietary fiber minerals and proteins. The corms of Colocasia antiquorum contain anthocyanins such as pelargonidin‐3‐glucoside, cyanidin‐3‐glucoside, and cyanidin‐3‐chemnoside, which are reported to possess antifungal and antioxidative characteristics. The underground corms of taro (Colocasia esculenta), which contain 70%–80% starch, are the primary reason for its cultivation. Taro is a highly digestible root vegetable with a high content of mucilaginous gums and trivial starchy granules. It is used to make a variety of dishes. This review article highlights the functional properties, phytochemical profile, encapsulating properties, and various industrial applications. Its health advantages and dietary uses were also addressed.

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Effects of extraction methods on the digestibility, cytotoxicity, prebiotic potential and immunomodulatory activity of taro (Colocasia esculenta) water-soluble non-starch polysaccharide

Cited by 14 publications

References 50 publications

Effects of Taro (Colocasia esculenta) Water-Soluble Non-Starch Polysaccharide, Lactobacillus acidophilus, Bifidobacterium breve, Bifidobacterium infantis, and Their Synbiotic Mixtures on Pro-Inflammatory Cytokine Interleukin-8 Production

Effects of Taro (Colocasia esculenta) Water-Soluble Non-Starch Polysaccharide, Lactobacillus acidophilus, Bifidobacterium breve, Bifidobacterium infantis, and Their Synbiotic Mixtures on Pro-Inflammatory Cytokine Interleukin-8 Production

Structural Elucidation and Immunostimulatory Activities of Quinoa Non-starch Polysaccharide Before and After Deproteinization

Functional profile and encapsulating properties of Colocasia esculenta (Taro)

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